Bisphenol-A impairs cellular function and alters DNA methylation of stress pathway genes in first trimester trophoblast cells

Basak, Sanjay; Vilasagaram, Srinivas; Duttaroy, Asim K.

doi:10.1016/j.reprotox.2018.10.009

Cited by 45 publications

(37 citation statements)

References 42 publications

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“…Bisphenol-A (BPA): Merck et al [5] showed that BPA is transported through the placenta and increases β-HCG, thus affecting placental growth [6]. In following studies, others proved that, in the first trimester of pregnancy, the amount of BPA in trophoblast cells was proven to interfere with cellular growth and to affect DNA methylation [32].…”

Section: From Environment To Pregnant Mothermentioning

confidence: 99%

Endocrine-Disrupting Chemicals in Human Fetal Growth

Street

Bernasconi

2020

IJMS

113

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Fetal growth is regulated by a complex interaction of maternal, placental, and fetal factors. The effects and outcomes that chemicals, widely distributed in the environment, may have on the health status of both the mother and the fetus are not yet well defined. Mainly mixtures of chemical substances are found in the mothers and placenta. Exposure to endocrine-disrupting chemicals (EDCs) can be associated with fetal growth retardation, thyroid dysfunction, and neurological disorders. EDCs mostly interfere with insulin, glucocorticoid, estrogenic, and thyroid pathways, with subsequent effects on normal endocrine and metabolic functions, which cause changes in the epigenome and state of inflammation with life-long effects and consequences. International scientific societies recommend the implementation of research and of all possible preventive measures. This review briefly summarizes all these aspects.

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Section: From Environment To Pregnant Mothermentioning

confidence: 99%

Endocrine-Disrupting Chemicals in Human Fetal Growth

Street

Bernasconi

2020

IJMS

113

View full text Add to dashboard Cite

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“…Cells (5 × 10 3 /well/100 µL) were suspended in 5% FBS‐RPMI and seeded homogeneously using 12‐channel pipette and loading reservoir. The assay was performed using the protocol described before (Basak et al, 2018b) except incubation with assay media for 24 h. Optical density (OD) was recorded at 562 nm using Synergy H1 hybrid multimode reader (Biotek Instrument, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Cellular angiogenesis was measured in vitro by measuring tube length as the index of tube formation capacity in the presence of assay media by following the protocol described previously except a few modifications (Basak et al, 2018b). Both HTR8/SVneo and HMEC‐1 cells were pre‐starved in FBS free, growth factor free RPMI, and MCDB131 media, respectively for 48 h prior to their seeding in matrigel containing complete media.…”

Section: Methodsmentioning

confidence: 99%

“…Gene expression was measured by quantifying mRNA expression of the target genes and housekeeping gene (TBP) using the ABI7900HT system as described before (Basak et al, 2018b). Predesigned SYBR green I primers (#KSPQ12012; Sigma) were used (Table S1) for this purpose.…”

Section: Methodsmentioning

confidence: 99%

“…Promoter DNA methylation of genes associated with human metabolic and oxidative stress was performed using Epitect PCR array kit (#335222 EAHS‐3580ZE; Qiagen) as described before (Basak et al, 2018b). In this method, each sample with an equal amount of genomic DNA (2 µg) was digested with restriction enzymes in four separate setups consists of mock (control) digest (Mo), methylation‐sensitive (Ms), methylation‐dependent (Md), and double digests (Msd).…”

Section: Methodsmentioning

confidence: 99%

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Curcumin stimulates angiogenesis through VEGF and expression of HLA‐G in first‐trimester human placental trophoblasts

Basak

Vilasagaram

Mallepogu

et al. 2020

Cell Biology International

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Curcumin has a protective role in placental diseases like preeclampsia and preterm birth. Very little is known about its functional effects on growth, angiogenesis, and epigenetic activities of human first trimester placenta. HTR8/SVneo trophoblasts cells were used as model for human first trimester placenta. Effects of curcumin (≥80%) in these cells were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), radioactive thymidine uptake, quantitative real-time polymerase chain reaction (qRT-PCR), promoter DNA methylation, qRT-PCR array, tube formation, wound healing, and immunoblot assays. PC3 (prostate cancer), JEG-3 (trophoblast), and HMEC-1 (endothelial) cells were used as control in various experiments. Unlike in PC3 cells, curcumin stimulated growth, proliferation, and viability in HTR8/SVneo cells. Curcumin increased tube formation, and messenger RNA (mRNA) expression of angiogenic factors such as vascular endothelial growth factor A (VEGFA) and protein expression of proangiogenic factor VEGF receptor-2 and fatty acid-binding protein-4 (FABP4) in these cells. Curcumin-stimulated tube formation was associated with an increased expression of VEGFR2 and FABP4. The stimulatory effects of curcumin were inhibited by VEGFR2 (SU5416) and FABP4 (BMS309403) inhibitors. Curcumin also significantly increased both mRNA and protein expression of HLA-G in HTR8/ SVneo cells. Curcumin increased mRNA expression of DNMT3A and NOTCH signaling system whereas down-regulated mRNA expression of HSD11β2. Curcumin enhanced hypomethylation of gene promoters against oxidative stress and DNA damage pathway mediators. Curcumin promotes cell growth, migration, and thus angiogenic potential of these cells. Increased expression of HLA-G by curcumin, hitherto unknown, is a novel finding since HLA-G not only favors the immune environment for invasive trophoblasts but also positively modulates angiogenesis.

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Plastics derived endocrine‐disrupting compounds and their effects on early development

Basak

Das

Duttaroy

2020

Birth Defects Research

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115

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Despite the fact that the estrogenic effects of bisphenols were first described 80 years ago, recent data about its potential negative impact on birth outcome parameters raises a strong rationale to investigate further. The adverse health effects of plastics recommend to measure the impacts of endocrine-disrupting compounds (EDCs) such as bisphenols (BPA, BPS, BPF), bis(2-ethylhexyl) phthalate, and dibutyl phthalate (DBP) in human health. Exposure to these compounds in utero may program the diseases of the testis, prostate, kidney and abnormalities in the immune system, and cause tumors, uterine hemorrhage during pregnancy and polycystic ovary. These compounds also control the processes of epigenetic transgenerational inheritance of adult-onset diseases by modulating DNA methylation and epimutations in reproductive cells. The early developmental stage is the most susceptible window for developmental and genomic programming. The critical stages of the events for a normal human birth lie between the many transitions occurring between spermatogenesis, egg fertilization and the fully formed fetus. As the cells begin to grow and differentiate, there are critical balances of hormones, and protein synthesis. Data are emerging on how these plastic-derived compounds affect embryogenesis, placentation and feto-placental development since pregnant women and unborn fetuses are often exposed to these factors during preconception and throughout gestation. Impaired early development that ultimately influences fetal outcomes is at the center of many developmental disorders and contributes an independent risk factor for adult chronic diseases. This review will summarize the current status on the impact of exposure to plastic derived

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Bisphenol-A impairs cellular function and alters DNA methylation of stress pathway genes in first trimester trophoblast cells

Cited by 45 publications

References 42 publications

Endocrine-Disrupting Chemicals in Human Fetal Growth

Endocrine-Disrupting Chemicals in Human Fetal Growth

Curcumin stimulates angiogenesis through VEGF and expression of HLA‐G in first‐trimester human placental trophoblasts

Plastics derived endocrine‐disrupting compounds and their effects on early development

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