Bisphenol A induces apoptosis in central neural cells during early development of Xenopus laevis

Oka, Tomohiro; Adati, Naoki; Senoo, Tadashi; Sakuma, Kei; Nishimura, Takuya; Kurose, Kouichi

doi:10.1016/j.bbrc.2003.10.199

Cited by 52 publications

(39 citation statements)

References 25 publications

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“…On contrary in our study we studied with embryos and we could not see any apoptotic effects of low doses of BPA on zebrafish embryos. Similarly to our results, Oka et al [38] studied the effects of BPA on apoptosis in central neural cells during early development of Xenopus and they also couldn't observe apoptotic effects in early stages. …”

Section: Discussionsupporting

confidence: 88%

Effects of Low Doses of Bisphenol A on Primordial Germ Cells in Zebrafish (Danio rerio) Embryos and Larvae

Akbulut¹,

Kızıl²,

Yön³

2013

Kafkas Univ Vet Fak Derg

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SummaryPrimordial germ cells are the precursors of gametes in sexually reproducing organisms. The migration and homing process of primordial germ cells are prone to environmental effects and the endocrine system hormones. One of the environmental pollutants which humans are exposed to is bisfenol A (BPA). BPA which is an endocrine disrupter generally used for making plastics harder. In this study we investigated the effects of low doses of BPA (4 mg/L and 8 mg/L) on primordial germ cells at the zebrafish embryos and larvae. Whole mount in situ hybridization for germ cell marker gene vasa showed that upon exposure to BPA, the primordial germ cell number increased and these cells ectopically localized outside the normal germ cell niche in zebrafish. Migration of primordial germ cells into ectopic locations and morphological changes in these cells were also proved by histological studies. Additionally, results of acridine orange staining to detect apoptotic cells showed that low doses of BPA did not increase apoptosis. Results of the present study showed that low doses of BPA exposure increased the number of primordial germ cells and induced ectopic primordial germ cell localization in the zebrafish embryos. Keywords: Zebrafish, Primordial germ cell, Bisphenol A Düşük Dozlardaki Bisfenol A'nın Zebra Balığı (Danio rerio) Embriyo ve Larvalarında Primordiyal Germ Hücreleri Üzerine Etkisi ÖzetPrimordiyal germ hücreleri, eşeyli üreyen canlılarda gametlerin öncül hücreleridir. Endokrin sistem hormonları ve çevresel etkenler, primordiyal germ hücrelerinin göçünü etkilemektedir. İnsanların maruz kaldığı çevresel kirleticilerden biri de bisfenol A (BPA)'dır. Endokrin sistemde hasar oluşturan biri olan BPA, genellikle plastik sertleştirme işleminde kullanılmaktadır. Bu çalışmada zebra balığı embriyo ve larvalarında düşük dozlardaki (4 mg/L and 8 mg/L) BPA'nın etkileri incelenmiştir. Bir germ hücresi belirteci gen olan vasa ile yapılan whole mount in situ hibridizasyon deneyleri sonucunda BPA'nın primordiyal germ hücre sayısında artışa sebep olduğu ve bu hücrelerin göç yolu dışındaki (ektopik) bölgelerde bulunduğu tespit edilmiştir. Primordiyal germ hücrelerinin ektopik bölgelere göçü ve bu hücrelerdeki morfolojik değişiklikler histolojik çalışmalarla da doğrulanmıştır. Buna ek olarak, düşük dozlarıyla yapılan boyamalarda apoptozisin tespitinde kullanılan akridin turuncusu boyaması sonucunda BPA'nın apoptozisi artırmadığı saptanmıştır. Bu çalışma sonuçları, düşük dozlardaki BPA'nın primordiyal germ hücre sayısını artırdığını ve bu hücrelerin ektopik bölgelere göç etmesine yol açtığını ortaya koymaktadır.

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Section: Discussionsupporting

confidence: 88%

Effects of Low Doses of Bisphenol A on Primordial Germ Cells in Zebrafish (Danio rerio) Embryos and Larvae

Akbulut¹,

Kızıl²,

Yön³

2013

Kafkas Univ Vet Fak Derg

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“…Initially BpA was added at the stage when the egg was fertilized but almost all embryos died prior to neulura owing to an abnormality of the neural tube (data not shown). Oka et al 5) reported similar results. So, embryos were treated with BpA at stage 10.5.…”

Section: Treatment Of Xenopus Embryos With Bisphenol Amentioning

confidence: 56%

“…Xenopus eggs were obtained by injecting the females with 300 units of human chorionic gonadotropin, gestron (Denka Seiyaku, Japan), and were fertilized with testes isolated from Xenopus laevis males by surgical operation. Embryos were dejellied with 1% sodium thioglycollate and cultured in 0.1ϫMBS (Modified Birth's solution, [ 19) Bisphenol A (Wako Pure Chemical Industry, Tokyo, Japan) was dissolved in DMSO and added to the culture medium of Xenopus embryos in a final concentration of 25, 50, or 100 mM, according to the reports of Oka et al 5) and Iwamuro et al…”

Section: Eggs Embryos and Treatment Of Embryos Withmentioning

confidence: 99%

“…In the early development of Xenopus laevis, BpA has been shown to induce apoptosis in central nervous tissue cells of the brain and spinal cord. 5) In mice, prenatal and neonatal exposure to BpA induces a significant increase in the levels of dopamine D 1 receptor mRNA in the brain and enhances central dopamine D 1 receptor-mediated activity. 6) Despite these intriguing results, however, the molecular mechanism by which BpA induces these phenomena remains unclear.…”

mentioning

confidence: 99%

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Bisphenol A Causes Malformation of the Head Region in Embryos of Xenopus laevis and Decreases the Expression of the ESR-1 Gene Mediated by Notch Signaling

Imaoka

Mori

Kinoshita

2007

Biological & Pharmaceutical Bulletin

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“…Oka et al (2003), support not indicated, examined the effects of bisphenol A exposure on development of the frog Xenopus laevis. Embryos were exposed to the ethanol vehicle or 10-100 mM [2.3-23 mg/L] bisphenol A from developmental stage 6 until the early tadpole stage (late stage 10) [purity not indicated, and culture ware not discussed].…”

Section: Utility (Adequacy) For Cerhr Evaluation Processmentioning

confidence: 99%

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

Chapin

Adams

Boekelheide

et al. 2008

Birth Defects Research Pt B

404

304

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Bisphenol A induces apoptosis in central neural cells during early development of Xenopus laevis

Cited by 52 publications

References 25 publications

Effects of Low Doses of Bisphenol A on Primordial Germ Cells in Zebrafish (Danio rerio) Embryos and Larvae

Effects of Low Doses of Bisphenol A on Primordial Germ Cells in Zebrafish (Danio rerio) Embryos and Larvae

Bisphenol A Causes Malformation of the Head Region in Embryos of Xenopus laevis and Decreases the Expression of the ESR-1 Gene Mediated by Notch Signaling

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A

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