Bisulfite or sulfite inhibits growth of Helicobacter pylori

Abstract

Bisulfite or sulfite was found to be inhibitory to Helicobacter pyloni growth. A modified version of Brucella broth (BB), bisulfite-less BB (BLBB), supported rapid, robust, and consistent growth of H. pylori. We suggest that BLBB simply be called "Pylori broth" for distinction from Brucella broth. Helicobacter pylori, a gram-negative, spiral bacterium, has been implicated as the etiological agent for gastritis and as a major contributing factor in the development of peptic gastroduodenal ulcers (2, 5, 8, 9, 12, 13). It has also been linked to an increased risk of gastric cancer (3). Since its first isolation in 1983 (9, 17), H. pylori has generally been described as an oxygen-susceptible, fastidious microaerophile (1, 10, 11, 14-16). Growth media and incubation conditions that provide an environment of reduced oxygen tension have commonly been used for culturing H. pylori. Brucella broth (BB) is one of the frequently used media (10, 11, 14-16). The medium consists of rich carbon and nitrogen sources, such as tryptone, peptamin, yeast extract, and glucose, and two salts, sodium chloride and sodium bisulfite. Bisulfite is thought to be useful in reducing oxygen tension in the medium. In our earlier experience with BB, growth of H. pylori was consistently more robust in older medium which had been stored in the refrigerator than in freshly prepared medium. Since bisulfite (HS03 1) and sulfite (S-3) can gradually be oxidized to sulfate (SO4 2) on

Acidic Conditions Enhance Bactericidal Effects of Sodium Bisulfite on Helicobacter pylori

“…We reported the marked stimulation by hydrogen peroxide of the growth of H. pylori in Brucella broth medium. Hydrogen peroxide, which inactivated sodium bisulfite, promoted growth [5]. In the present study, we showed that the bactericidal effects of sodium bisulfite were stronger under acidic conditions than neutral conditions.…”

“…This medium is composed of rich carbon and nitrogen sources, tryptone, peptamine, yeast extract, glucose, sodium chloride, and sodium bisulfite; the latter compound is added to decrease oxygen content. Hawrylik et al reported that sodium bisulfite inhibits the growth of H. pylori [5]. We observed previously that the addition of a low concentration of hydrogen peroxide eliminates this effect [6].…”

Development and evaluation of a new growth medium forHelicobacter pylori

“…The effect of sodium sulfite added to the basic IHPA medium was a decrease in the number of colonies recovered, indicating that sodium sulfite was inhibitory to H. pylori growth. Our finding that sodium sulfite was inhibitory to H. pylori growth is comparable with the finding of other researchers (Hawrylik et al, 1994).…”

“…In our laboratory, commercial Eugon agar (CEA) was extensively used to culture H. pylori. However, there have been reports that sodium sulfite (Hawrylik et al, 1994), one of the ingredients of CEA, was inhibitory to some H. pylori strains. The present study was aimed at modifying the original formulation of CEA with a view to develop a new H. pylori growth medium and to evaluate the new growth medium for the growth of other H. pylori isolates.…”

Brefeldin A enhances Helicobacter pylori vacuolating cytotoxin-induced vacuolation of epithelial cells

“…The strains were sub-cultured every 1-2 days. For preparation of culture supernatants, strains were swabbed from 24 h growth plates into 12 ml sulphite-free brucella broth containing 0.2% (w/v) b-cyclodextrin [14], and grown in a gas jar shaking in a microaerobic environment generated by a CampyPak for 2-3 days at 37°C…”