Bivalent Aptasensor Based on Silver-Enhanced Fluorescence Polarization for Rapid Detection of Lactoferrin in Milk

Zhu, Chen; Li, Hui; Jia, Wenchao; Liu, Xiao‐Hui; Li, Zhoumin; Fang, Wen; Zheng, Nan; Jiang, Jindou; Xu, Danke

doi:10.1021/acs.analchem.7b00261

Cited by 66 publications

(47 citation statements)

References 41 publications

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“…In general, ternary amplification is based on Ag 10 NPs, split aptamers, and the MEF effect, all contributed to the significant increase of FP values. It was proved that the sensitivity of this assay was about 3 orders of magnitude over traditional aptamer-based homogeneous assays, with a detection limit of 1.25 pM [42].…”

Section: Intermolecular Switchmentioning

confidence: 95%

The Design and Improvement of Aptamer-based Fluorescent Probes

Zou

Zhao

et al. 2019

CSM

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Fluorescent probes exhibit the characteristics of high sensitivity, diversified design, and strong quantitative analysis ability. Therefore, they have become one of the research hotspots in analytical chemistry and biological analysis. Aptamers are single-stranded DNAs or RNAs that are obtained in vitro and can selectively bind to the targets, making them ideal candidates as identifying units in fluorescent probes. However, most of the current aptamer probes are not regulatable because their fluorescence signals can't be changed after the fluorescent probes are bound to the target, resulting in a high signal background and limited contrast. Actually, the structure of aptamers is flexible and can be combined with a variety of nanomaterials to achieve high sensitivity fluorescence for quantitative analysis, and can be used for a variety of target determinations, such as metal ions, small molecules, proteins and cells. In addition, with the development of research on DNA nanotechnology, researchers have designed many aptamer-based fluorescent probes with special properties, which have good application prospects in complex sample analyses and even in vivo cell imaging. In this review, the development of research in the field of aptamer-based fluorescent probes is surveyed and four mechanisms of aptamer fluorescent probes, including dye intercalation, conversion of DNA conformation, signal amplification, and mediation of nanomaterials, are comprehensively discussed.

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Section: Intermolecular Switchmentioning

confidence: 95%

The Design and Improvement of Aptamer-based Fluorescent Probes

Zou

Zhao

et al. 2019

CSM

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“…[64]) and (b) bivalent aptasensor using sliver decahedral nanoparticles for enhanced detection of lactoferrin in milk (reprinted with permission from ref. [77]). LG using aptamer-functionalized graphene screen-printed electrodes (reprinted with permission from ref.…”

Section: Aptasensorsmentioning

confidence: 99%

“…A novel bivalent aptasensor based on fluorescence polarization has been reported for lactoferrin detection by Chen et al [77]. The fluorescence polarization is an effective technique where high sensitivity to the rate of rotation can be achieved by target-induced conformational or structural changes.…”

Section: Aptasensorsmentioning

confidence: 99%

Nano-Biosensing Platforms for Detection of Cow’s Milk Allergens: An Overview

Nehra

Lettieri

Dilbaghi

et al. 2019

Sensors

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Among prevalent food allergies, cow milk allergy (CMA) is most common and may persist throughout the life. The allergic individuals are exposed to a constant threat due to milk proteins’ presence in uncounted food products like yogurt, cheese, and bakery items. The problem can be more severe due to cross-reactivity of the milk allergens in the food products due to homologous milk proteins of diverse species. This problem can be overcome by proper and reliable food labeling in order to ensure the life quality of allergic persons. Therefore, highly sensitive and accurate analytical techniques should be developed to detect the food allergens. Here, significant research advances in biosensors (specifically immunosensors and aptasensors) are reviewed for detection of the milk allergens. Different allergic proteins of cow milk are described here along with the analytical standard methods for their detection. Additionally, the commercial status of biosensors is also discussed in comparison to conventional techniques like enzyme-linked immunosorbent assay (ELISA). The development of novel biosensing mechanisms/kits for milk allergens detection is imperative from the perspective of enforcement of labeling regulations and directives keeping in view the sensitive individuals.

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“…The equilibrium dissociation constants (K d ) of YFL-1, YFL-4, YFL-5 and YFL-6 were 1.04 AE 0.50 nM, 0.953 AE 0.11 nM, 8.91 AE 1.29 nM and 4.97 AE 0.46 nM, respectively. 33,36 All these values were detected by SPR and based on the formula K d ¼ K off /K on . The K d values of these four aptamers were in the low nanomolar range, from 0.953 nM to 8.91 nM, demonstrating the strong binding of these aptamers to Lac.…”

Section: Analysis Of Aptamer Sequencesmentioning

confidence: 99%