Bivariate and multivariate analyses of the correlations between stability of the erythrocyte membrane, serum lipids and hematological variables

Neto, Morun Bernardino; Avelar, Erick; Arantes, Thiago Silva; Jordão, I.A.; Huss, Juliana Carla da Costa; Souza, Tatiana Maria T. de; Penha, V.A. de Souza; Silva, S.C. da; Souza, Paulo César A. de; Tavares, Marcelo; Penha-Silva, Nilson

doi:10.3233/bir-130641

Cited by 14 publications

(19 citation statements)

References 54 publications

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“…The graphs of A 540 as a function of the NaCl (X) concentration were adjusted by sigmoidal nonlinear regression, according to the Boltzmann equation: where A max and A min represent respectively the maximum and minimum plateaus of A 540 , H 50 is the concentration of NaCl capable of promoting 50% hemolysis and dX represents one-fourth of the variation in NaCl concentration responsible for promoting 100% hemolysis [20,24]. The saline concentration at the initial point of the curve defines the variable H 0 , which is the saline concentration required to initiate in vitro hemolysis, and can be calculated by the formula H 0 = H 50 + 4dX/2.…”

Section: Methodsmentioning

confidence: 99%

“…For the good performance of its functions, the erythrocyte must remain intact. The red blood cell's ability to maintain the physical and chemical integrity of its membrane in adverse situations is called stability [20,21]. In this sense, the ability of the biological membrane to resist fragmentation in the face of mechanical aggression promoted by the blood flow itself and the friction with the wall of the blood vessels constitutes the so-called mechanical stability, while its ability to remain intact due to volume expansion in a hyposmotic environment is called osmotic stability [22].…”

Section: Introductionmentioning

confidence: 99%

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The role of the erythrocyte in the outcome of pregnancy with preeclampsia

et al. 2019

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The objective of this study was to analyze the relationships of osmotic and mechanical stability of erythrocytes with anthropometric, biochemical, hematologic and hemodynamic variables in pregnant women with preeclampsia (PE). The studied population consisted of 20 normotensive patients and 16 patients with PE. Patients with PE presented worse gestational outcome, greater hematologic impairment, erythrocytes osmotically more stable in vitro , but in conditions of isotonicity with the in vivo medium, in addition to hyperflow in orbital territory, when compared to normotensive patients. The correlation analysis between anthropometric, hematologic and hemodynamic variables in patients with PE indicated that erythrocytes with lower volumes and lower levels of hemoglobin favor the occurrence of a better gestational outcome, because they are more stable and because they are associated with a decrease in the hemodynamic changes present in the disease. This should mean that the tendency to microcytosis, probably due to a mechanism of compensatory mechanical selection, is a desirable characteristic in the disease.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The role of the erythrocyte in the outcome of pregnancy with preeclampsia

et al. 2019

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“…The graphs of A540 as a function of the NaCl ( X ) concentration were adjusted by nonlinear sigmoidal regression, according to the Boltzmann equation:

where A max and A min represent, respectively, the maximum and minimum plateaus of A 540 , H 50 is the concentration of NaCl capable of promoting 50% hemolysis, and dX represents 1/4 of the change in NaCl concentration responsible for 100% hemolysis [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…In this study, the possible association of alterations in erythrocyte behavior with PE will be investigated through the analysis of osmotic stability. The analysis of the erythrocyte behavior in a hyposmotic gradient is a powerful method that reflects the deformability [ 28 ], stability [ 29 , 30 ], and the membrane composition of these cells, allowing the investigation of the influences of the lipid blood level [ 31 ] oxidative aggression [ 26 ], among other factors. Although there are already studies on the osmotic stability of erythrocytes in both pregnancy and PE, they are usually presented as a percentage of hemolysis as a function of time [ 32 ] and lysis ratio at specific salt concentration [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Are There Differences in the Anthropometric, Hemodynamic, Hematologic, and Biochemical Profiles between Late- and Early-Onset Preeclampsia?

Freitas

Costa

Medeiros

et al. 2018

Obstetrics and Gynecology International

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Preeclampsia (PE) is classified as early-onset PE (EOPE) and late-onset PE (LOPE) when present before or after 34 weeks of gestation, respectively. This transversal study aimed to investigate the differences and possible associations existing in the anthropometric, hemodynamic, hematologic, and biochemical profiles of late- and early-onset preeclampsia. The study included 65 volunteers admitted to a tertiary hospital in Brazil: 29 normotensive and 36 with preeclampsia (13 with EOPE and 23 with LOPE). Pregnant women with LOPE presented greater weight gain and borderline increase in body mass index at the end of gestation in relation to the other groups, which is compatible with the metabolic origin, associated with obesity, attributed to this form of the disease. Pregnant women with EOPE presented a borderline reduction in the number of erythrocytes and a significant decrease in the number of platelets, in addition to a significant increase in reticulocytes, serum iron, and ferritin when compared to normotensive pregnant women and pregnant women with LOPE. A significant increase in osmotic stability of erythrocytes was observed in the EOPE group in relation to other groups. Hemodynamic analysis by Doppler ultrasonography of the ophthalmic artery showed that both groups of pregnant women with PE presented alterations compatible with the occurrence of hyperflow in the orbital territory. These hemodynamic changes were associated with changes in hematimetric indices.

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“…The stability can be defined as the membrane's ability to resist lysis against many harmful agents. Many factors that affect the stability of the membrane are well known; among them are diet, age, temperature, use of drugs and some physiological and pathological conditions [ 13 – 17 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of acute and chronic exercise on the osmotic stability of erythrocyte membrane of competitive swimmers

et al. 2017

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This study aimed to evaluate the influence of acute and chronic exercise on erythrocyte membrane stability and various blood indices in a population consisting of five national-level male swimmers, over 18 weeks of training. The evaluations were made at the beginning and end of the 1st, 7th, 13th and 18th weeks, when volume and training intensity have changed. The effects manifested at the beginning of those weeks were considered due to chronic adaptations, while the effects observed at the end of the weeks were considered due to acute manifestations of the exercise load of that week. Acute changes resulting from the exercise comprised increases in creatine kinase activity (CK) and leukocyte count (Leu), and decrease in hematocrit (Ht) and mean corpuscular volume (MCV), at the end of the first week; increase in the activities of CK and lactate dehydrogenase (LDH), in the uric acid (UA) concentration and Leu count, at the end of the seventh week; increases in CK and LDH activities and in the mean corpuscular hemoglobin concentration (MCHC), at the end of the 13th week; and decrease in the value of the osmotic stability index 1/H50 and increases in the CK activity and platelets (Plt) count, at the end of the 18th week. Chronic changes due to training comprised increase in the values of 1/H50, CK, LDH, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), serum iron (Fe), MCV and Plt. Although acute training has resulted in decrease in the osmotic stability of erythrocytes, possibly associated with exacerbation of the oxidative processes during intense exercise, chronic training over 18 weeks resulted in increased osmotic stability of erythrocytes, possibly by modulation in the membrane cholesterol content by low and high density lipoproteins.

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Bivariate and multivariate analyses of the correlations between stability of the erythrocyte membrane, serum lipids and hematological variables

Cited by 14 publications

References 54 publications

The role of the erythrocyte in the outcome of pregnancy with preeclampsia

The role of the erythrocyte in the outcome of pregnancy with preeclampsia

Are There Differences in the Anthropometric, Hemodynamic, Hematologic, and Biochemical Profiles between Late- and Early-Onset Preeclampsia?

Effects of acute and chronic exercise on the osmotic stability of erythrocyte membrane of competitive swimmers

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