Blastocyst culture and transfer: analysis of results and parameters affecting outcome in two in vitro fertilization programs

Schoolcraft, William B.; Gardner, David K.; Lane, Marcus B.; Schlenker, Terry; Hamilton, Fredesminda; Meldrum, David R.

doi:10.1016/s0015-0282(99)00311-8

Cited by 274 publications

(140 citation statements)

References 20 publications

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“…Blastocysts were evaluated by two embryologists who assigned each blastocyst a grade using the grading system of Schoolcraft et al [14]. Embryos were cryopreserved on either day 5 or 6, depending on the grade of the blastocyst morphology.…”

Section: Ovarian Stimulationmentioning

confidence: 99%

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Itoi¹,

Asano²,

Shimizu³

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of this study is to examine the clinical outcomes of blastocysts derived from human singlepronucleate (1PN) embryos after conventional in vitro fertilization (cIVF) and intracytoplasmic sperm injection (ICSI) cycles. Methods This was a retrospective study at a reproductive center of a hospital. To evaluate embryo quality and clinical outcomes, cIVF or ICSI cycles with one or more 1PN embryos were compared with same cycles with 2PN embryos (control cycles).Results A total of 623 cycles (426 cIVF cycles and 197 ICSI cycles) were treated with cIVF or ICSI. The single pronuclear status rate was similar between cIVF (22.1 %) and ICSI (25.1 %) cycles. Although the development rates of 1PN embryos on day 3 and day 5/6 in cIVF were significantly higher than those in ICSI, those of 1PN embryos in cIVF were significantly lower compared to 2PN embryos (p<0.01). Nonetheless, the ongoing pregnancy rates achieved with 1PN blastocysts in 1PN embryos did not significantly differ from the control group. Thirty-three transfer cycles with 33 blastocysts derived from 1PN embryos in cIVF resulted in nine deliveries with no newborn malformations; however, no implantation was observed in three ICSI cycles. Conclusion Although the blastocyst formation rate of 1PN embryos was significantly lower than 2PN embryos in cIVF and ICSI cycles, 1PN blastocysts in cIVF, and not from ICSI, demonstrated an adequate ongoing pregnancy rate. These results suggested that 1PN blastocysts in cIVF are available for clinical use and may lead to an increase in the chance of pregnancy in patients receiving assisted reproductive technology with 1PN embryos.

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Section: Ovarian Stimulationmentioning

confidence: 99%

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Itoi¹,

Asano²,

Shimizu³

et al. 2015

J Assist Reprod Genet

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show abstract

“…Therefore, the advent of culture methods that permit the production and growth of embryos in vitro has facilitated widespread use of these methods (Gardner and Lane, 1998;Schoolcraft et al, 1999). Many assisted reproductive technologies make use of in vitro matured oocytes and cultured embryos; examples include ICSI, embryo culture out to the blastocyst stage, embryo transfer, as well as fertilization with normal or X-or Y-sorted sperm Greve and Callesen, 2004).…”

Section: Introductionmentioning

confidence: 99%

Acid peptidase activity released from in vitro produced porcine embryos: A candidate marker to predict developmental competence

Telugu

Spate

Prather

et al. 2008

Molecular Reproduction Devel

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SUMMARYThe ability to efficiently create high quality embryos, competent to produce normal viable offspring in vitro, facilitates diverse technological advancements in animal agriculture and assisted reproduction. Current methods for evaluation of embryos are predominantly based on morphological characteristics which are prone to potential bias of the scorer. Metabolic and genetic markers have also been explored for quality assessment, but they are cost prohibitive or require longer periods of time for evaluation. We hypothesized that secreted enzymes could provide another means of embryo quality assessment. In this report, we provide evidence that medium conditioned by porcine embryos often has proteolytic activity that operates in acidic conditions (acid peptidase activity or APA). The APA could be inhibited by pepstatin A, suggesting that the activity is derived from one or more aspartic peptidases. We also provide evidence that single embryos, incubated for as few as 24 hr, released enough APA that it was possible to measure it accurately at day 5 of culture. We also observed that such activity on day 6 could be positively correlated with advanced developmental stage and embryo quality. In addition, those embryos that were graded identically by morphological evaluations often differed in the amount of APA-with some being significantly higher than the experimental threshold value. Therefore, the APA of embryos might serve as an additional marker for evaluation of embryos.

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“…These advanced stage embryos have been reported, based on retrospective comparative studies, to be associated with higher per embryo implantation 1 rates when compared to day-3 embryos (2). As a result, it has been proposed that pregnancy rates can be maintained with fewer embryos transferred per cycle (3). The advantage of this strategy is thought to be the potential to lower the rate of multiple gestations, especially higher-order pregnancies.…”

Section: Introductionmentioning

confidence: 99%

Monozygotic Twins and Triplets in Association with Blastocyst Transfer

Jain

Boostanfar

Slater

et al. 2004

J Assist Reprod Genet

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Purpose : To compare the incidence of monozygotic twins following blastocyst versus day-3 embryo transfer (ET). Methods : A retrospective analysis of the outcome of assisted reproductive technology (ART) cycles utilizing blastocyst ET during 1999-2000 was compared to a similar group of patients undergoing day-3 ET during 1997-1998. Results : Blastocyst ET was used in 75 cycles with 2.0 ± 2 embryos transferred. The comparison group consisted of 90 cycles with day-3 ET and 3.0 ± 2 embryos transferred. Conclusions : High pregnancy rates are maintained with blastocyst ET even though fewer embryos are transferred. The rate of monozygotic twins is higher with blastocyst ET than with day-3 ET. This increase may partially negate the benefit of reduced high-order multiple gestations attributed to blastocyst ET.

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Blastocyst culture and transfer: analysis of results and parameters affecting outcome in two in vitro fertilization programs

Cited by 274 publications

References 20 publications

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Acid peptidase activity released from in vitro produced porcine embryos: A candidate marker to predict developmental competence

Monozygotic Twins and Triplets in Association with Blastocyst Transfer

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