Blastocyst is the source of prostaglandins in the implantation site in the rabbit

Pakrasi, Pranab L.; Dey, Sudhansu K.

doi:10.1016/0090-6980(82)90178-2

Cited by 54 publications

(18 citation statements)

References 8 publications

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“…Considerable circumstantial evidence has accumulated suggesting that prostaglandins may be essential for the initiation of implantation. Increased concentrations of prostaglandins have been found in endometrial tissue adjacent to the implanting blastocyst (Kennedy & Zamecnik, 1978), prostaglandins have been found in the pre¬ implantation blastocyst (Dickmann & Spilman, 1975;Pakrasi & Dey, 1982;Harper et al, 1983) and pretreatment of pregnant rats with indomethacin abolishes both increased vascular per¬ meability and the decidual response as well as prevents successful implantation (Tobert, 1976; Kennedy, 1979Kennedy, , 1985Phillips & Poyser, 1981;Kennedy & Lukash, 1982). In the rabbit, the role of prostaglandins is not as well defined; treatment of pregnant does with indomethacin reduces the number of implanting blastocysts and delays the onset of implantation and par¬ turition, but does not completely ablate the nidatory process (Hoos & Hoffman, 1980;Jones et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Platelet-activating factor in the rabbit uterus during early pregnancy

Angle

Jones

McManus³

et al. 1988

Reproduction

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Summary. Platelet-activating factor (PAF) concentrations were low in the non-pregnant, oestrous uterus (mean \m=+-\s.e.m.: 2\m=.\2\m=+-\1\m=.\2 pmol/g, n = 3). However, uterine PAF increased dramatically during pregnancy to a maximum of 37\m=.\8\ m=+-\ 4\m=.\90pmol/g (n = 7) on Day 5. By Day 7, PAF concentrations in the uteri of pregnant rabbits had returned to levels similar to those found at oestrus. In contrast, uterine PAF in pseudopregnant rabbits peaked at 30\m=.\6\ m=+-\2\m=.\8pmol/g (n = 8) on Day 4, declined to 20\m=.\5\ m=+-\ 2\ m=. \ 4pmol/g (n = 8) on Day 5 and then remained at that concentration through Day 7. Uterine PAF co-migrated with synthetic PAF 1-0-hexadecyl-2-acetyl-sn-glycero-phosphocholine) in both thin-layer and normal-phase high-performance liquid chromatography. PAF activity in the uterus during pregnancy and pseudopregnancy was found almost exclusively in the endometrium; little or no PAF was found in myometrium, uterine flushings or blastocysts. While no PAF was detected in blastocysts on Days 5 and 6 of pregnancy, the presence of the embryo appears to modulate biosynthesis and/or degradation of PAF by the uterus, since PAF decreased significantly in uterine tissue apposed to the implanting embryo (but not in similar areas between such attachment sites). Increased concentrations of PAF in the preimplantation rabbit uterus followed by a dramatic decrease on the day of blastocyst attachment suggest that this potent inflammatory autacoid may play a vital role in implantation.

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Section: Introductionmentioning

confidence: 99%

Platelet-activating factor in the rabbit uterus during early pregnancy

Angle

Jones

McManus³

et al. 1988

Reproduction

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“…They detected, by radioimmunoassay (RIA), PGE-A and PGF in rabbit blastocysts 144 h post coitum and suggested that the embryos had the ability to synthesize the PGs. PGs have since been detected by RIA in blastocysts of rabbits (Dey et ai, 1980;Pakrasi & Dey, 1982;Harper et ai, 1983), cows (Shemesh et ai, 1979;Lewis et ai, 1982), ewes (Lacroix & Kann, 1982;Hyland et ai, 1982) and sows (Davis et ai, 1983).…”

Section: Introductionmentioning

confidence: 99%

“…While the exact function of the embryonic PGs is unknown, PGs in blastocysts have been considered to participate in the process of implantation (Pakrasi & Dey, 1982). It has also been reported that PGE-2 has a major role in the hatching of mouse blastocysts (Baskar et ai, 1981) and is able to induce implantation of mouse blastocysts (Holmes & Gordashko, 1980 (Whittingham, 1971); 24 h after hCG injection to obtain pronuclear embryos; 48 h for 2-cell embryos; 60 h for 4 cell embryos; 67 h for 8-cell embryos; 80 h for morulae and 96 h for blastocysts.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical demonstration of prostaglandin E-2 in preimplantation mouse embryos

Niimura

Ishida²

1987

Reproduction

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Summary. An antiserum to prostaglandin (PG) E-2 and indirect immunofluorescence were used to demonstrate immunohistochemically the presence of PGE-2 in preimplantation mouse embryos. Fluorescence was observed in the cytoplasm of unfertilized 1-cell embryos to the blastocyst stage. The strongest fluorescence was detected at the 8-cell and morula stages. In embryos cultured from the 2-cell stage on, the fluorescence was observed in the cytoplasm of 4-cell embryos to the blastocyst stage. No differences were observed in the intensity and the distribution of the fluorescence between embryos in vivo and those in vitro. However, when blastocysts were cultured in a medium containing 100 \g=m\m-indomethacin, the fluorescence was diminished markedly. We therefore suggest that preimplanted mouse embryos contain PGE-2 during their early developmental stages and that the embryos synthesize the PGE-2.

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“…This proposal was based on the facts that prostaglandins have been detected in the rabbit blastocyst (Dickman & Spilman, 1975), that antagonists of prostaglandin synthesis or actions inhibit swelling and hatching of the blastocyst in vitro, and that ample evidence from studies on other epithelia implicate prostaglandins in the transport of fluid (see Biggers et al 1978b, for a review). Since then it has been shown that 7 day old rabbit blastocysts can synthesize prostaglandins (Dey, Chien, Cox & Crist, 1980) and that the blastocyst is the main source of prostaglandins in the implantation site (Pakrasi & Dey, 1982). Furthermore Racowsky & Biggers (1983) have demonstrated the endogenous synthesis of PGE2, PGF2a, PGA and thromboxane B2 from the phospholipid pool by 6 day rabbit blastocysts.…”

Section: Effect Of K+-free Mediamentioning

confidence: 99%

Sodium and chloride co‐transport by preimplantation rabbit blastocysts.

Benos¹,

Biggers²

1983

The Journal of Physiology

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SUMMARY1. Influxes of Na+ and C1-across the trophectodermal epithelium of 6 day post coitum (p.c.) rabbit blastocysts are partially interrelated. This relationship was investigated under conditions in which either the external [Na+] or [Cl-] concentration was varied. Our results indicate that an ouabain-insensitive, furosemide-sensitive, Na+: Cl-co-transport system exists in the 6 and 6'5 day p.c. rabbit embryo, but not in the 5 day p.c. blastocyst.2. There was no dependence of NaCl uptake on the presence of K+ in the bathing medium.3. No evidence ofNa+/H+ or Cl-/HCO3-exchange systems in the rabbit blastocyst was found.4. The effects of furosemide, exogenous cyclic AMP, and various exogenous prostaglandins on Na+ and Cl-movements were studied. Cyclic AMP and/or prostaglandin E1 may regulate the operation of this Na+ :Cl-co-transport system.

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Blastocyst is the source of prostaglandins in the implantation site in the rabbit

Cited by 54 publications

References 8 publications

Platelet-activating factor in the rabbit uterus during early pregnancy

Platelet-activating factor in the rabbit uterus during early pregnancy

Immunohistochemical demonstration of prostaglandin E-2 in preimplantation mouse embryos

Sodium and chloride co‐transport by preimplantation rabbit blastocysts.

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