Blastocyst mitochondrial DNA (mtDNA) is not affected by oocyte vitrification: a sibling oocyte study

Arnanz, Ana; Munck, Neelke De; Bayram, A; El-Damen, Ahmed; Abdalla, Andrea; Elkhatib, Ibrahim; Melado, Laura; Lawrenz, Barbara; Fatemi, Human M.

doi:10.1007/s10815-020-01795-6

Cited by 10 publications

(11 citation statements)

References 50 publications

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“…Our results are consistent with the majority of studies, which were performed using cells from the blastocyst stage, showing that there the amount of mtDNA within aneuploid embryos is higher when compared to euploid embryos [7,12,13,17,18,[20][21][22][23][24]. We cannot directly base conclusions regarding mitochondrial activity on these results, as it can be an indirect indication of higher energy needs of aneuploid embryos possibly stemming from the initiated repair mechanisms.…”

Section: Discussionsupporting

confidence: 88%

“…As most of published studies concerning mtDNA copy number in human embryos were conducted using blastocysts from frozen IVF cycles [7][8][9][10][11][12][13]36], we decided to evaluate the end point parameters by assessing blastomeres biopsied at the cleavage stage that were transferred on day 5 of the same cycle. We aimed to evaluate the significance of the energy production process at such an early stage of embryo development.…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, several studies have been performed to evaluate mtDNA as a potential biomarker of embryo vitality. However, there is a lot of controversy as studies performed have reached contradicting conclusions [4,[6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial DNA Copy Number in Cleavage Stage Human Embryos—Impact on Infertility Outcome

Podolak¹,

Liss

Kiewisz

et al. 2022

CIMB

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A retrospective case control study was undertaken at the molecular biology department of a private center for reproductive medicine in order to determine whether any correlation exists between mitochondrial DNA (mtDNA) content of cleavage-stage preimplantation embryos and their developmental potential. A total of 69 couples underwent IVF treatment (averaged women age: 36.5, SD 4.9) and produced a total of 314 embryos. A single blastomere was biopsied from each embryo at the cleavage stage (day-3 post-fertilization) subjected to low-pass next generation sequencing (NGS), for the purpose of detecting aneuploidy. For each sample, the number of mtDNA reads obtained after analysis using NGS was divided by the number of reads attributable to the nuclear genome. The mtDNA copy number amount was found to be higher in aneuploid embryos than in those that were euploid (mean mtDNA ratio ± SD: 6.3 ± 7.5 versus 7.1 ± 5.8, p < 0.004; U Mann–Whitney test), whereas no statistically significant differences in mtDNA content were seen in relation to embryo morphology (6.6 ± 4.8 vs. 8.5 ± 13.6, p 0.09), sex (6.6 ± 4.1 vs. 6.2 ± 6.8, p 0.16), maternal age (6.9 ± 7.8 vs. 6.7 ± 4.5, p 0.14) or its ability to implant (7.4 ± 6.6 vs. 5.1 ± 4.6, p 0.18). The mtDNA content cannot serve as a useful biomarker at this point in development. However, further studies investigating both quantitative and qualitative aspects of mtDNA are still required to fully evaluate the relationship between mitochondrial DNA and human reproduction.

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Section: Discussionsupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mitochondrial DNA Copy Number in Cleavage Stage Human Embryos—Impact on Infertility Outcome

Podolak¹,

Liss

Kiewisz

et al. 2022

CIMB

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show abstract

“…Even now, there are a lot of controversies as conducted studies have reached conflicting results (Table 1). Most study groups assessing the relationship of mtDNA content and embryo's ploidy status agree that increased levels of mtDNA correlate with aneuploidy [38,73,76,78,87,89]. Nevertheless, the mechanisms leading to such an effect are still not known.…”

Section: Assessment Of Mtdna Content In Human Embryos and Its Clinica...mentioning

confidence: 99%

“…Divergent results were also obtained for assessment of the relationship of the mitochondrial score with embryos' morphology and especially maternal age [38,[73][74][75][76][77][78][79][80][81][82][83][84][85][86][87][88][89][90][91].…”

Section: Assessment Of Mtdna Content In Human Embryos and Its Clinica...mentioning

confidence: 99%

The Role of Mitochondria in Human Fertility and Early Embryo Development: What Can We Learn for Clinical Application of Assessing and Improving Mitochondrial DNA?

Podolak

Wocławek-Potocka

Łukaszuk

2022

Cells

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Mitochondria are well known as ‘the powerhouses of the cell’. Indeed, their major role is cellular energy production driven by both mitochondrial and nuclear DNA. Such a feature makes these organelles essential for successful fertilisation and proper embryo implantation and development. Generally, mitochondrial DNA is exclusively maternally inherited; oocyte’s mitochondrial DNA level is crucial to provide sufficient ATP content for the developing embryo until the blastocyst stage of development. Additionally, human fertility and early embryogenesis may be affected by either point mutations or deletions in mitochondrial DNA. It was suggested that their accumulation may be associated with ovarian ageing. If so, is mitochondrial dysfunction the cause or consequence of ovarian ageing? Moreover, such an obvious relationship of mitochondria and mitochondrial genome with human fertility and early embryo development gives the field of mitochondrial research a great potential to be of use in clinical application. However, even now, the area of assessing and improving DNA quantity and function in reproductive medicine drives many questions and uncertainties. This review summarises the role of mitochondria and mitochondrial DNA in human reproduction and gives an insight into the utility of their clinical use.

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Does blastocyst mitochondrial DNA content affect miscarriage rate in patients undergoing single euploid frozen embryo transfer?

El-Damen,

Elkhatib,

Bayram

et al. 2021

J Assist Reprod Genet

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Blastocyst mitochondrial DNA (mtDNA) is not affected by oocyte vitrification: a sibling oocyte study

Cited by 10 publications

References 50 publications

Mitochondrial DNA Copy Number in Cleavage Stage Human Embryos—Impact on Infertility Outcome

Mitochondrial DNA Copy Number in Cleavage Stage Human Embryos—Impact on Infertility Outcome

The Role of Mitochondria in Human Fertility and Early Embryo Development: What Can We Learn for Clinical Application of Assessing and Improving Mitochondrial DNA?

Does blastocyst mitochondrial DNA content affect miscarriage rate in patients undergoing single euploid frozen embryo transfer?

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