Blastocyst transfer after extended culture of cryopreserved cleavage embryos improves in vitro fertilization cycle outcomes

Lê, Minh Tâm; Nguyen, Thai Thanh Thi; Nguyen, Trung Van; Dang, Hong Nhan Thi; Nguyen, Quoc Huy Vu

doi:10.1016/j.cryobiol.2021.04.003

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…Whether the subset that does successfully implant after additional culture represents PM embryos with a PrE that is able to "catch up" during extended culture is an interesting question but will require the development of methods to assess the state of the PrE in live embryos before transfer to answer. Further, that extended culture does not rescue implantation for the majority of PM embryos in these studies suggests that defects in most PM embryos constitute more than a simple delay [63][64][65] .…”

Section: Discussionmentioning

confidence: 75%

“…For the subset of PM embryos in which PrE development is delayed, embryo culture could be prolonged and transfer postponed to better match the timing of PrE development with the optimal window of endometrial receptivity. In support of this possibility, some studies have demonstrated that extended culture of PM embryos before transfer can increase implantation rates for a subset of PM blastocysts [63][64][65] . Whether the subset that does successfully implant after additional culture represents PM embryos with a PrE that is able to "catch up" during extended culture is an interesting question but will require the development of methods to assess the state of the PrE in live embryos before transfer to answer.…”

Section: Discussionmentioning

confidence: 96%

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Molecular signatures associated with successful implantation of the human blastocyst

Cook-Andersen

Chousal

Srinivasan

et al. 2023

Preprint

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Embryo implantation in humans is remarkably inefficient for reasons that remain largely unexplained, and high rates of implantation failure remain one of the greatest obstacles in treating infertility. The volume of gene expression data available from human embryos has rapidly accumulated in recent years. However, prioritization of these data to identify the subset of genes that determine successful implantation remains a challenge, in part, because comprehensive analyses cannot be performed on the same embryos that are transferred. Here, we leverage clinical morphologic grading—known for decades to correlate with implantation potential—and transcriptome analyses of matched embryonic and abembryonic samples to identify genes and cell-cell interactions enriched and depleted in human blastocysts of good and poor morphology, genome-wide. Unexpectedly, we discovered that the greatest molecular difference was in the state of the extraembryonic primitive endoderm (PrE), with relative deficiencies in PrE development in embryos of poor morphology at the time of embryo transfer. Together, our results support a model in which implantation success is most strongly reflected by factors and signals from the embryonic compartment and suggest that deficiencies in PrE development, in particular, are common among embryos with reduced implantation potential. Our study provides a valuable resource for those investigating the markers and mechanisms of human embryo implantation.

show abstract

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 96%

Molecular signatures associated with successful implantation of the human blastocyst

Cook-Andersen

Chousal

Srinivasan

et al. 2023

Preprint

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“…IVF consists of a manual fertilization procedure in which embryos are cultured in an incubator for 3-5 days until they reach the blastocyst stage [4]. The best embryos (blastocysts) are selected based on valuable morphological attributes and transferred back to the patient's uterus [5,6].…”

Section: Introductionmentioning

confidence: 99%

Detecting Blastocyst Components by Artificial Intelligence for Human Embryological Analysis to Improve Success Rate of In Vitro Fertilization

Arsalan

Haider

Choi

et al. 2022

JPM

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Morphological attributes of human blastocyst components and their characteristics are highly correlated with the success rate of in vitro fertilization (IVF). Blastocyst component analysis aims to choose the most viable embryos to improve the success rate of IVF. The embryologist evaluates blastocyst viability by manual microscopic assessment of its components, such as zona pellucida (ZP), trophectoderm (TE), blastocoel (BL), and inner cell mass (ICM). With the success of deep learning in the medical diagnosis domain, semantic segmentation has the potential to detect crucial components of human blastocysts for computerized analysis. In this study, a sprint semantic segmentation network (SSS-Net) is proposed to accurately detect blastocyst components for embryological analysis. The proposed method is based on a fully convolutional semantic segmentation scheme that provides the pixel-wise classification of important blastocyst components that help to automatically check the morphologies of these elements. The proposed SSS-Net uses the sprint convolutional block (SCB), which uses asymmetric kernel convolutions in combination with depth-wise separable convolutions to reduce the overall cost of the network. SSS-Net is a shallow architecture with dense feature aggregation, which helps in better segmentation. The proposed SSS-Net consumes a smaller number of trainable parameters (4.04 million) compared to state-of-the-art methods. The SSS-Net was evaluated using a publicly available human blastocyst image dataset for component segmentation. The experimental results confirm that our proposal provides promising segmentation performance with a Jaccard Index of 82.88%, 77.40%, 88.39%, 84.94%, and 96.03% for ZP, TE, BL, ICM, and background, with residual connectivity, respectively. It is also provides a Jaccard Index of 84.51%, 78.15%, 88.68%, 84.50%, and 95.82% for ZP, TE, BL, ICM, and background, with dense connectivity, respectively. The proposed SSS-Net is providing a mean Jaccard Index (Mean JI) of 85.93% and 86.34% with residual and dense connectivity, respectively; this shows effective segmentation of blastocyst components for embryological analysis.

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Molecular profiling of human blastocysts reveals primitive endoderm defects among embryos of decreased implantation potential

Chousal,

Morey,

Srinivasan

et al. 2024

Cell Reports

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Blastocyst transfer after extended culture of cryopreserved cleavage embryos improves in vitro fertilization cycle outcomes

Cited by 4 publications

References 24 publications

Molecular signatures associated with successful implantation of the human blastocyst

Molecular signatures associated with successful implantation of the human blastocyst

Detecting Blastocyst Components by Artificial Intelligence for Human Embryological Analysis to Improve Success Rate of In Vitro Fertilization

Molecular profiling of human blastocysts reveals primitive endoderm defects among embryos of decreased implantation potential

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