Blastocystis subtypes in cancer patients: Analysis of possible risk factors and clinical characteristics

Yersal, Özlem; Malatyalı, Erdoğan; Ertabaklar, Hatice; Oktay, Esin; Barutça, Sabri; Ertuğ, Sema

doi:10.1016/j.parint.2016.02.010

Cited by 42 publications

(38 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, overall infection rates of E. bieneusi and Blastocystis were 1.3% (5/381) and 7.1% (27/381), respectively. Both of them were lower than those in previous reports from cancer patients, with E. bieneusi in Mexico (40%, 4/10), Turkey 23.7% (22/93), and Iran (1.7%, 4/234), and Blastocystis in France (16%, 15/94), Malaysia (21.1%, 43/204), and Turkey (10.8%, 25/232) (Poirier et al, 2011 ; Jiménez-González et al, 2012 ; Kumarasamy et al, 2014 ; Hamamc1 et al, 2015 ; Mirjalali et al, 2015 ; Yersal et al, 2016 ). In fact, infection rates of these two pathogens vary from country to country and within different communities of the same country.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, there have been a few reports of microsporidia and Blastocystis infections in cancer patients in Iran, Mexico, Malaysia, France, and Turkey, with genotypes D and E of E. bieneusi and ST1-ST7 of Blastocystis being identified (Lono et al, 2008 ; Tan et al, 2009 ; Poirier et al, 2011 ; Chandramathi et al, 2012 ; Jiménez-González et al, 2012 ; Mirjalali et al, 2015 ; Yersal et al, 2016 ). In China, no studies involve E. bieneusi infection in cancer patients and no molecular data are available on Blastocystis infection in this population.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genotyping of Enterocytozoon bieneusi and Subtyping of Blastocystis in Cancer Patients: Relationship to Diarrhea and Assessment of Zoonotic Transmission

et al. 2017

View full text Add to dashboard Cite

Enterocytozoon bieneusi (E. bieneusi) and Blastocystis are common pathogens responsible for diarrhea in humans, especially in immunocompromised individuals. The number of cancer patients has been increasing and diarrhea is a common clinical symptom in the treatment of cancers. To understand the prevalences and genotypes/subtypes of E. bieneusi and Blastocystis in cancer patients in China, to track the infection sources, and to explore the relationships between E. bieneusi and Blastocystis infections and diarrhea, 381 fecal specimens were collected from cancer patients. Each of them was analyzed for the presence of E. bieneusi and Blastocystis by PCR amplifying and sequencing the ITS region of the rRNA gene and the barcode region of the SSU rRNA gene, respectively. 1.3 and 7.1% of cancer patients were positive for E. bieneusi and Blastocystis, respectively. No statistical differences were observed in the infection rates between the groups by age, gender, and residence. E. bieneusi and Blastocystis were both significantly more common in cancer patients with diarrhea, and significant relationship of Blastocystis to diarrhea was found in chemotherapy group. Two E. bieneusi genotypes (D and a novel one named as HLJ-CP1) and two Blastocystis subtypes (ST1 and ST3) were identified with three novel ST1 sequences. This is the first report of occurrence and molecular characterizations of E. bieneusi and Blastocystis in cancer patients in China. E. bieneusi genotype D and Blastocystis ST1 and ST3 have been identified in humans and animals while one novel E. bieneusi genotype falling into zoonotic group 1, implying a potential of zoonotic transmission.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genotyping of Enterocytozoon bieneusi and Subtyping of Blastocystis in Cancer Patients: Relationship to Diarrhea and Assessment of Zoonotic Transmission

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Doenças infecciosas, particularmente aquelas causadas por patógenos endêmicos, merecem especial atenção, pois apresentam risco considerável para pacientes imunocomprometidos 37,51 , sobretudo, aqueles, que receberam transplante de órgãos sólidos 51,52 . Com o aumento do número de transplantes e o desenvolvimento de drogas imunossupressoras mais potentes, a frequência de rejeição tem diminuído, mas, por outro lado os receptores tornaram-se suscetíveis a infecções oportunistas 51,52. A incidência e prevalência de infecções parasitárias em receptores de transplantes são pouco conhecida 51 .…”

Section: 9unclassified

“…para facilitar a identificação tem sido recomendado as colorações permanentes, e/ou os métodos de cultura 36,73 . Por outro lado, tem sido relatado que alguns subtipos não apresentam bom crescimento em determinados meios de cultura 13,37 .…”

Section: Critério De Exclusãounclassified

“…De acordo com Marcos e Gotuzzo 80 , pacientes com HIV/Aids e transplantados são mais suscetíveis a desenvolverem os sintomas associados com presença de Blastocystis sp. Neste contexto, a diversidade genética e a resposta imune do hospedeiro podem ser fatores importantes para o desenvolvimento da infecção37,50 A análise molecular baseada no gene SSU-rDNA tem apontado 17 subtipos de Blastocystis, encontrados em humanos, outros mamíferos e aves5 . Os subtipos ST7 são comumente detectados em aves na Ásia e no Oriente Médio11 .…”

unclassified

See 1 more Smart Citation

Detecção de DNA de Blastocystis sp. em pacientes candidatos a transplantes atendidos no Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP)

Silva¹

View full text Add to dashboard Cite

Blastocystis sp. é um protozoário intestinal comumente encontrado em amostras fecais de muitas espécies animais, incluindo humanos, sendo pouco estudado em pacientes imunocomprometidos, especialmente nos candidatos a transplante. O objetivo do presente estudo foi avaliar a ocorrência e a identificação molecular de subtipos (ST) de Blastocystis sp. em amostras fecais de pacientes candidatos a transplante. A Reação em Cadeia da Polimerase foi realizada utilizando primers específicos para o DNA ribossomal de Blastocystis. As sequências de DNA obtidas foram alinhadas e comparadas com outras sequências do banco de dados GenBank e MLST (Multilocus Sequence Typing). As amostras analisadas mostraram uma positividade de 16% (24/150) para Blastocystis sp. A maior ocorrência foi observada em candidatos a transplante renal (31,4%), seguida de candidatos a transplante hepático (10,4%) e candidatos a transplante de medula óssea (5,9%). O ST3 (45,8%) foi o mais prevalente entre os isolados, seguido pelo ST1 (37,5%), ST2 (12,5%) e ST7 (4,2%). Foi observado dentro dos subtipos: os alelos 4 e 78/81 (ST1); alelos 11 e 12 (ST2); alelos 34, 36, 37 e 54 (ST3) e alelo 96 (ST7). Este é o primeiro estudo de identificação molecular de Blastocystis sp. em candidatos a transplante. Os presentes resultados confirmam a maior ocorrência do subtipo 3 em candidatos a transplante, além de reforçar a importância de novas investigações de Blastocystis sp. nesses pacientes.

show abstract

Intestinal helminths and protozoan infections in patients with colorectal cancer: prevalence and possible association with cancer pathogenesis

Toychiev¹,

Abdujapparov

Imamov

et al. 2018

Parasitol Res

View full text Add to dashboard Cite

The purpose of the present study was to determine the prevalence of intestinal helminths and protozoa in colorectal cancer (CRC) patients and to evaluate the possible association between the prevalence and CRC pathogenesis. A total of 200 CRC patients and 200 residents of Tashkent, who had no complaints related to the gastrointestinal tract, were examined by triple coproscopy using a concentration method and estimations of protozoan infection intensity. Of the CRC patients tested, 144 were classified as T-NM (without metastases) and 56 were classified as TNM (with metastases). Parasitological examination was performed during CRC diagnosis before and after surgery and chemotherapy. A significantly higher prevalence of Blastocystis sp., Chilomastix mesnili, Jodamoeba butschlii, and Endolimax nana was found in CRC patients than in the control population (p < 0.0001), amounting to 80, 20, 22.5, and 11.5%, respectively. The high prevalence of Blastocystis sp., as well as the patterns of infection intensity, was stable at all stages of examination. The ratio of the number of CRC patients with and without Blastocystis sp. in the TNM and TNM groups amounted to 3.3 and 7.0, respectively. The ratios for C. mesnili, E. coli, J. butschlii, and E. nana in both groups were 0.2 and 0.2, 0.07 and 0.07, 0.3 and 0.16, and 0.18 and 0.01, respectively. The prevalence of helminths and Giardia lamblia in CRC patients and the control population was not significantly different. Taken together, these data indicate a possible role for Blastocystis sp. in CRC pathogenesis. Diagnosis, treatment, and further observation of patients with Blastocystis sp. are necessary at all stages of CRC, including during diagnosis and before and after surgery and chemotherapy.

show abstract

Blastocystis subtypes in cancer patients: Analysis of possible risk factors and clinical characteristics

Cited by 42 publications

References 36 publications

Genotyping of Enterocytozoon bieneusi and Subtyping of Blastocystis in Cancer Patients: Relationship to Diarrhea and Assessment of Zoonotic Transmission

Genotyping of Enterocytozoon bieneusi and Subtyping of Blastocystis in Cancer Patients: Relationship to Diarrhea and Assessment of Zoonotic Transmission

Detecção de DNA de Blastocystis sp. em pacientes candidatos a transplantes atendidos no Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP)

Intestinal helminths and protozoan infections in patients with colorectal cancer: prevalence and possible association with cancer pathogenesis

Contact Info

Product

Resources

About