Blautia faecicola sp. nov., isolated from faeces from a healthy human

Abstract

An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean. Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae ( Clostridium rRNA cluster XIVa). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia . The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids. KGMB01111T contained meso-diaminopimelic acid in cell-wall peptidoglycan. The predominant end product of fermentation produced by KGMB01111T was acetic acid. Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp. nov. is proposed. The type strain is KGMB01111T (=KCTC 15706T=DSM 107827T).

Blautia intestinalis sp. nov., isolated from human feces

“…At the time of writing, the genus Blautia comprises 21 species and 15 validly published species with correct names (www.bacterio.net/Blautia.html). The validly published names are Blautia argi [4], Blautia caecimuris [5], Blautia coccoides [3, 6], Blautia faecicola [7], Blautia faecis [8], Blautia glucerasea [9], Blautia hansenii [3, 10], Blautia hominis [11], Blautia hydrogentrophica [3, 12], Blautia luti [3, 13] , Blautia obeum [14, 15], Blautia producta [3, 10], Blautia schinkii [3, 16], Blautia sterocoris [17] and Blautia wexlerae [3]. B.…”

Blautia liquoris sp. nov., isolated from the mud in a fermentation cellar used for the production of Chinese strong-flavour liquor

“…The genus Blautia belongs to the family Lachnospiraceae and was first proposed by Liu et al [1]. At the time of writing, the genus includes 14 species with validly published names: Blautia argi [2], Blautia caecimuris [3], Blautia coccoides [1], Blautia faecicola [4], Blautia faecis [5], Blautia hansenii [1], Blautia hominis [6], Blautia hydrogenotrophica [1], Blautia luti [1], Blautia obeum [7], Blautia producta [1], Blautia schinkii [1], Blautia stercoris [8] and Blautia wexlerae [1]. The above species have been isolated from human faeces [1, 4, 5, 7, 8], dog faeces [2] and mouse gut [3], respectively.…”

“…At the time of writing, the genus includes 14 species with validly published names: Blautia argi [2], Blautia caecimuris [3], Blautia coccoides [1], Blautia faecicola [4], Blautia faecis [5], Blautia hansenii [1], Blautia hominis [6], Blautia hydrogenotrophica [1], Blautia luti [1], Blautia obeum [7], Blautia producta [1], Blautia schinkii [1], Blautia stercoris [8] and Blautia wexlerae [1]. The above species have been isolated from human faeces [1, 4, 5, 7, 8], dog faeces [2] and mouse gut [3], respectively. Members of the genus Blautia are Gram-positive, non-motile, coccoid or oval-shaped, and obligate anaerobes that produce acetate, ethanol, hydrogen, lactate and succinate as the end products of glucose fermentation [1].…”

“…¶Data from [35]. #Data from [36] DNA G+C contents of members of the genus Blautia were mainly 37-45 mol%, the DNA G+C content of LZLJ-3 T was 42.1 mol%, which was within the range of DNA G+C content of the genus Blautia, but the DNA G+C content of strains Eubacterium cellosolvens 6 T , Fusicatenibacter saccharivorans HT 03-11 *Data taken from [4]. †Summed features are fatty acids that cannot be resolved reliably from another fatty acid using the chromatographic conditions chosen.…”

Table_1.XLSX

“…At this date, there are 17 officially accepted Blautia species, in addition to other 9 proposed so far, isolated from the GI tract from human and other mammals, including ruminants (Supplementary Table 1) (Liu et al, 2008;Furuya et al, 2010;Park et al, 2012Park et al, , 2013Lawson and Finegold, 2015;Lagkouvardos et al, 2016;Durand et al, 2017;Shin et al, 2018;Paek et al, 2019;Ghimire et al, 2020;Kim et al, 2020). In addition, there are nine proposed species "Blautia marasmi," "B. phocaeensis, " "B. provencensis, " "B. intestinalis, " "B. segnis, " "B. tardus, " "B. celeris, " "B. lentus" and "B. difficilis" (Pham et al, 2017a,b;Traore et al, 2017;Liu et al, 2020).…”