⿿Blind⿿ targeting in action: From phage display to breast cancer cell targeting with peptide-gold nanoconjugates

Galbiati, Elisabetta; Gambini, Luca; Civitarese, Viola; Bellini, Melissa; Ambrosini, Dario; Allevi, Raffaele; Avvakumova, Svetlana; Romeo, Sergio; Prosperi, Davide

doi:10.1016/j.phrs.2016.06.007

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…THP–gold nanoconjugates actively targeted MCF-7 cells in comparison to nontumor 3T3-L1 fibroblast cells . THPs specific for MCF-7 cells were selected from a phage display library, synthesized, and conjugated to spherical gold nanoparticles by a heterobifunctional cross-linker with an ethylene oxide spacer.…”

Section: Chimeric Compounds and Receptor-mediated Endocytosismentioning

confidence: 99%

“…118,119 THP−gold nanoconjugates actively targeted MCF-7 cells in comparison to nontumor 3T3-L1 fibroblast cells. 120 THPs specific for MCF-7 cells were selected from a phage display library, synthesized, and conjugated to spherical gold nanoparticles by a heterobifunctional cross-linker with an ethylene oxide spacer. This work proved the possibility of developing nanomaterials that can rely on tumor targeting potential irrespective of a specific knowledge of the target cell biology.…”

Section: Chimeric Compounds Andmentioning

confidence: 99%

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Designing Chimeric Molecules for Drug Discovery by Leveraging Chemical Biology

et al. 2020

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After the first seed concept introduced in the 18th century, different disciplines have attributed different names to dual-functional molecules depending on their application, including bioconjugates, bifunctional compounds, multitargeting molecules, chimeras, hybrids, engineered compounds. However, these engineered constructs share a general structure: a first component that targets a specific cell and a second component that exerts the pharmacological activity. A stable or cleavable linker connects the two modules of a chimera. Herein, we discuss the recent advances in the rapidly expanding field of chimeric molecules leveraging chemical biology concepts. This Perspective is focused on bifunctional compounds in which one component is a lead compound or a drug. In detail, we discuss chemical features of chimeric molecules and their use for targeted delivery and for target engagement studies.

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Section: Chimeric Compounds and Receptor-mediated Endocytosismentioning

confidence: 99%

Section: Chimeric Compounds Andmentioning

confidence: 99%

Designing Chimeric Molecules for Drug Discovery by Leveraging Chemical Biology

et al. 2020

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“…Additionally, the components of such devices combine the features of Nature’s own nanodevices (proteins) such as specific recognition, biocompatibility, and cooperativity with the electronic, magnetic, and optical properties of nanomaterials [1]. Lots of nanomaterials with peptide-formed monolayers have been obtained, including silver [2,3], gold [4], carbon nanomaterials [5,6], magnetic nanoparticles [7,8] and silica nanoparticles [9]. These materials show advantages such as high biocompatibility and high stability in various applications, for example, as chemical sensors [10], biosensors [11], in enzyme activity analysis [12,13], bio-imaging [14], drug delivery [9], diagnosis and therapy [4,6,8] and nano-architecture fabrication [15,16].…”

Section: Introductionmentioning

confidence: 99%

One Step Preparation of Peptide-Coated Gold Nanoparticles with Tunable Size

et al. 2019

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Gold nanoparticles (AuNPs) made from self-assembling peptides have been used in many research fields and attracted a great deal of attention due to their high stability, biocompatibility and functionality. However, existing preparation methods for peptide-coated AuNPs are post-synthesis processes, which are complicated and time consuming. Therefore, a one-step preparation method for peptide-coated AuNPs is proposed here. The AuNPs obtained by this method exhibit good stability. Importantly, peptide-coated AuNPs with precise different sizes can be prepared by this method through pH control of reducing reagent tyrosine in range of 10.0~12.7. Thus, the one-step preparation method proposed here provides a significant tool for the research in different fields concerning NP size, stability and biocompatibility.

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“…Active targeting to specific tissues or cell types can be achieved by incorporating high-affinity species (e.g. peptides) within the ligand shell of a GNP construct [17][18][19] . For cancer therapy, such nanoparticle constructs are intended to enable the selective delivery of a pharmaceutical ingredient (often a cytotoxin) to tumour tissues, thus sparing healthy tissues and minimising off-target toxicities.…”

Section: Introductionmentioning

confidence: 99%