Blister pouches for effective reagent storage on microfluidic chips for blood cell counting

Smith, Suzanne; Sewart, René; Becker, Holger; Roux, Pieter; Land, Kevin

doi:10.1007/s10404-016-1830-2

Cited by 32 publications

(22 citation statements)

References 29 publications

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“…Counting analysis on cells or particles can also be realized in wearable microfluidic devices . Javanmard et al combined parallel electrodes of the sensor with microfluidic channels in their wearable microfluidic impedance cytometer (Figure B).…”

Section: General Functions and Designs Of Microfluidics In Wearable Smentioning

confidence: 99%

Application of Microfluidics in Wearable Devices

et al. 2019

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Wearable devices integrated with various electronic modules, biological sensors, and chemical sensors have drawn large public attention. Due to their inherent advantages of superior stretchability, elaborate microstructure, high integration of multiple functions, and low cost, microfluidics are an excellent candidate and have already been widely used in wearable devices. Well‐designed microfluidic devices can realize excellent multiple functions in wearable devices, including sample collecting, handling and storage, sample analysis, signal converting and amplification, mechanic sensing, and power supplying. Moreover, the microfluidic wearable devices with further integration of wireless modules have exhibited potential applications in healthcare monitoring, clinical assessment, and human and intelligent device interaction. This review focuses on the latest advances on multifunctional wearable devices based on microfluidics, primarily including general functions and designs of microfluidic wearable devices, and their specific applications in physiological signal monitoring, clinical diagnosis and therapeutics, and healthcare.

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Section: General Functions and Designs Of Microfluidics In Wearable Smentioning

confidence: 99%

Application of Microfluidics in Wearable Devices

et al. 2019

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“…In the simplest version of the chip, the sample loading, wash, and fill steps can be done manually with a pipette. Alternatively, blister packs or pouches containing water and buffer solutions can be attached (with fluid connection) to chip and actuated by finger, or mechanical or electromechanical actuators to affect the wash and filling steps [ 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. Similar approaches include chips with integrated flexible polymer films [ 56 ] or foils [ 57 ].…”

Section: Microfluidic Molecular Diagnosticsmentioning

confidence: 99%

Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

et al. 2018

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Designs and applications of microfluidics-based devices for molecular diagnostics (Nucleic Acid Amplification Tests, NAATs) in infectious disease testing are reviewed, with emphasis on minimally instrumented, point-of-care (POC) tests for resource-limited settings. Microfluidic cartridges (‘chips’) that combine solid-phase nucleic acid extraction; isothermal enzymatic nucleic acid amplification; pre-stored, paraffin-encapsulated lyophilized reagents; and real-time or endpoint optical detection are described. These chips can be used with a companion module for separating plasma from blood through a combined sedimentation-filtration effect. Three reporter types: Fluorescence, colorimetric dyes, and bioluminescence; and a new paradigm for end-point detection based on a diffusion-reaction column are compared. Multiplexing (parallel amplification and detection of multiple targets) is demonstrated. Low-cost detection and added functionality (data analysis, control, communication) can be realized using a cellphone platform with the chip. Some related and similar-purposed approaches by others are surveyed.

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“…Recently, a variety of microfluidic devices have been proposed and demonstrated to perform cell lysis. Cell lysis techniques, based on mechanical, biological, and chemical methods, applied to microfluidic devices have been widely investigated [ 8 , 9 , 10 , 11 , 12 ]. Mechanical methods of cell lysing, such as sonication or freeze-thaw cycles, are the most common ways to lyse cells based on their nature, but these methods are most likely to break DNA [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Lab-on-a-Disc Platform for Automated Chemical Cell Lysis

Seo

Yoo

2018

Sensors

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Chemical cell lysis is an interesting topic in the research to Lab-on-a-Disc (LOD) platforms on account of its perfect compatibility with the centrifugal spin column format. However, standard procedures followed in chemical cell lysis require sophisticated non-contact temperature control as well as the use of pressure resistant valves. These requirements pose a significant challenge thereby making the automation of chemical cell lysis on an LOD extremely difficult to achieve. In this study, an LOD capable of performing fully automated chemical cell lysis is proposed, where a combination of chemical and thermal methods has been used. It comprises a sample inlet, phase change material sheet (PCMS)-based temperature sensor, heating chamber, and pressure resistant valves. The PCMS melts and solidifies at a certain temperature and thus is capable of indicating whether the heating chamber has reached a specific temperature. Compared to conventional cell lysis systems, the proposed system offers advantages of reduced manual labor and a compact structure that can be readily integrated onto an LOD. Experiments using Salmonella typhimurium strains were conducted to confirm the performance of the proposed cell lysis system. The experimental results demonstrate that the proposed system has great potential in realizing chemical cell lysis on an LOD whilst achieving higher throughput in terms of purity and yield of DNA thereby providing a good alternative to conventional cell lysis systems.

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Blister pouches for effective reagent storage on microfluidic chips for blood cell counting

Cited by 32 publications

References 29 publications

Application of Microfluidics in Wearable Devices

Application of Microfluidics in Wearable Devices

Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests

Lab-on-a-Disc Platform for Automated Chemical Cell Lysis

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