Blocking hsa_circ_0074027 suppressed non-small cell lung cancer chemoresistance via the miR-379-5p/IGF1 axis

Abstract

Cancer cell chemoresistance is the primary reason behind cancer treatment failure. Previous reports suggest that circular RNA (circRNA) hsa_circ_0074027 (HC0074027) is a crucial modulator of non-small cell lung cancer (NSCLC) disease progression. Herein, we delineated the underlying mechanism of HC0074027-regulated chemoresistance in NSCLC. We employed quantitative real-time polymerase chain reaction (qRT-PCR) or Elisa in the detection of HC0074027, micoRNA-379-5p (miR-379-5p), and insuline-like growth factor I (IGF1) expressions. Cell survival was evaluated via the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Direct associations among HC0074027, miR-379-5p, and IGF1 were examined via dual-luciferase reporter (DLR) and RNA immunoprecipitation (RIP) assays. Lastly, HC0074027 was incorporated into nude mice to examine its biological activity in vivo. Based on our analysis, HC0074027 levels strongly correlated with NSCLC chemoresistance to docetaxel (DTX), cisplatin (DDP), and paclitaxel (PTX). Alternately, HC0074027 silencing enhanced chemosensitivity in vitro. In vivo, HC0074027 downregulation suppressed tumor expansion and increased cancer cell sensitivity to chemotherapy. We also revealed that HC0074027 physically interacts with miR-379-5p to exert its biological function in vitro. Moreover, IGF1 is a functionally crucial target of miR-379-5p in modulating NSCLC chemoresistance in vitro. Finally, we

Current evidence on circRNAs as potential theranostic markers for detecting chemoresistance in breast cancer: a systematic review and meta‑analysis

“…Therefore, circRNAs have the chance to be excellent biomarkers for BC diagnosis, prognosis and drug resistance assessment 3,33,48,49 . Some studies have concentrated on the effects of circRNAs on chemoresistance in breast, cervical 50 , colorectal 51,52 , gastric 53,54 , lung 55 , oral 56 , ovarian 57 , pancreatic 58 and prostate 59 cancers. In this study, we collected relevant articles before 25 October 2021 and conducted a systematic review and meta-analysis, hoping to find clues about the value of circRNAs as biomarkers for BC prognosis.…”

CircCEP85 upregulates IGF1 expression to promote breast cancer progression via sponging miR-1193

“…IGF-1 is a polypeptide hormone belonging to the growth factor hormone family that helps control the growth and development of organs, muscles, and tissues in the body, besides, it is also involved in regulating glucose metabolism and brain function, IGF-1 assists with natural bodily development, while abnormal accumulation of IGF-1 can negatively affect health [25]. Currently, IGF1 was found to be involved in many cancers, such as lung cancer [26], cervical cancer [27], pancreatic cancer [28], and esophageal carcinoma [29]. In breast cancer, IGF-1 gene polymorphism was associated with an increased risk for this cancer [30].…”

Circular RNAs in chemotherapy resistance of lung cancer and their potential therapeutic application

“…The activation of IGF1/IGF1R and AKT signaling pathway that has been reported play significant roles in the progression and chemo‐resistance of cancer 85 . circ_0074027 could sequester miR‐379‐5p and increased the IGF‐1 expression, and then activated IGF1 signaling pathway to promote docetaxel, cisplatin, and paclitaxel resistance of NSCLC 86 . circ‐CDR1‐AS contributed to pemetrexed and cisplatin chemoresistance through the activation of phosphatidylinositol 3‐kinase and protein kinase B (Akt).…”