Blocking of the Lymphocyte Antigen Receptor Site with Anti-immunoglobulin Sera in vitro

Warner, Noel L.; Byrt, Pauline; Ada, G. L.

doi:10.1038/226942a0

Cited by 122 publications

(50 citation statements)

References 3 publications

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“…Receptors for antigen on the surfaces of B lymphocytes are generally believed to be antibody molecules (15). However, findings of Pernis (16) indicate that membrane-fluorescing antibody in some B cells is not of the same class as cytoplasmic fluorescing antibody, suggesting a possible distinction between receptor antibody and secreted antibody.…”

Section: Discussionmentioning

confidence: 77%

Antibody Responses to Antigenic Determinants of Influenza Virus Hemagglutinin

Virelizier

Allison

Schild

1974

The Journal of Experimental Medicine

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The "original antigenic sin" phenomenon (OAS) 1 challenges the dogma of the specificity of the immunological memory: when there is sequential infection with two different but antigenically related strains of influenza A virus, the antibody stimulated by the second infection reacts more strongly with the primary virus than with the one actually eliciting the response (1-3). This phenomenon is now well documented with many viral and nonviral cross-reacting antigens (4-8). However, its study with influenza antigens is of special interest because of its wide implications in the sero-epidemiology of influenza and the response to vaccination. In the accompanying paper (9) we have shown that purified hemagglutinin (HA) extracted from related influenza viruses share cross-reacting antigenic determinants, but differ in strain-specific determinants. We have now analyzed the antibody response to each of these groups of determinants after sequential exposure of mice to two related HA's, and have carried out cell-transfer experiments in an attempt to elucidate the cellular mechanisms responsible for the aberrant immunological recall of the OAS phenomenon. Ha, unrelated hemagglutinin; HA, purified hemagglutinin; Ho, homologous; OAS, original antigenic sin; Sl, strain-specific antibodies to H~ hemagglutinin; So, strain-specific antibodies to Ho hemagglutinin; SRDT, single radial diffusion tests; T ÷ mice, immunologically intact mice; TXBM mice, thymectomized, irradiated, and bone marrow reconstituted mice. Materials and Methods Animals, Immunization Schedules, Preparation of Purified-Concentrated Viruses

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Section: Discussionmentioning

confidence: 77%

Antibody Responses to Antigenic Determinants of Influenza Virus Hemagglutinin

Virelizier

Allison

Schild

1974

The Journal of Experimental Medicine

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“…There has been considerable interest in the immunoglobulin class specificity of these receptors in unprimed and in immunized animals. Antisera to various immunoglobulin determinants have been used to block the uptake of radiolabelled antigen by ABC formation (Warner, Byrt and Ada, 1970). A few papers have described inhibition by immunoglobulin antisera of rosette formation on 2 or 3 days of the immune response but results are equivocal (Biozzi, Binaghi, Stiffel and Mouton, 1969;McConnell, Munro, Cumer and Coombs, 1969;Creaves and Hogg, 1970).…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin Determinants on Rosette‐forming Cells: Their Changing Nature During an Immune Response

1971

Aust J Exp Biol Med

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Summary Antisera to mouse immunoglobulin heavy and light chains were used in vitro to inhibit the formation of rosettes in mouse spleen cell suspensions taken at intervals after sheep red blood cell immunization. Anti‐kappa produced greater than 90% inhibition of both immune and non‐immune rosettes. 65–80% inhibition of non‐immune and immune rosettes was induced by anti‐μ, up to 14 days after injection. Late in the response anti‐μ inhibited rosettes by less than 50%. Anti‐γ inhibited immune rosettes only, increasing to 42% inhibition on the 28th day of response. Anti‐α inhibited rosette formation only during the peak of the response. These results are discussed as relating to antigen‐binding receptors on rosette‐forming cells.

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mentioning

confidence: 99%

“…These receptors develop in fetal life before the animal is introduced to antigens and in this period are largely of the ~M immunoglobulin class (6,7). Combination of an antigen with these specific recognition units results in division and differentiation of many of these cells into mature antibody-secreting cells (plasma cells) (6,8). In general, the class of antibody secreted by these latter cells will be the same as the class of antibody present on the surface of the B lymphocytes from which these cells were derived (9,10).…”

mentioning

confidence: 99%

The effect of anti-mu suppression of gammaM and gammaG on the production of gammaE.

Dwyer

Rosenbaum

Lewis

1976

The Journal of Experimental Medicine

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Newbonr mice were treated from the day of birth with either bovine gamma globulin or anti-mu chain sera. The latter was administered using a protocol known to produce suppression of gammaM, gammaG, and gammaA production. Subsequent immunization with ovalbumin (OA) in alum was attempted to see if suppression of gammaM, gammaG, and gammaA classes of antibody would also be accompanied by suppression of gammaE-producing capacity. gammaG and gammaM antibody to OA and mercaptoethanol-resistant (gammaG) antibody to OA were measured by passive hemagglutination; gammaG and gammaE anti-OA antibodies were measured by passive cutaneous anaphylaxis. Anti-mu suppression was achieved with significant reduction in gammaM and gammaG antibodies. gammaE antibodies were not affected, suggesting an ontogenetic development for gammaE-bearing lymphocytes independent of the previously described gammaM to gammaG to gammaA ontogenetic sequence.

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Blocking of the Lymphocyte Antigen Receptor Site with Anti-immunoglobulin Sera in vitro

Cited by 122 publications

References 3 publications

Antibody Responses to Antigenic Determinants of Influenza Virus Hemagglutinin

Antibody Responses to Antigenic Determinants of Influenza Virus Hemagglutinin

Immunoglobulin Determinants on Rosette‐forming Cells: Their Changing Nature During an Immune Response

The effect of anti-mu suppression of gammaM and gammaG on the production of gammaE.

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