Immunoglobulin Determinants on Rosette‐forming Cells: Their Changing Nature During an Immune Response

Abstract

Summary Antisera to mouse immunoglobulin heavy and light chains were used in vitro to inhibit the formation of rosettes in mouse spleen cell suspensions taken at intervals after sheep red blood cell immunization. Anti‐kappa produced greater than 90% inhibition of both immune and non‐immune rosettes. 65–80% inhibition of non‐immune and immune rosettes was induced by anti‐μ, up to 14 days after injection. Late in the response anti‐μ inhibited rosettes by less than 50%. Anti‐γ inhibited immune rosettes only, increasing to 42% inhibition on the 28th day of response. Anti‐α inhibited rosette formation only during the peak of the response. These results are discussed as relating to antigen‐binding receptors on rosette‐forming cells.

Surface Immunoglobulins on the Thoracic Duct Lymphocytes of the Congenitally Athymic (Nude) Mouse

“…The observation of the superaddition of heavy chain labelling in excess of light chain labelling in the above experiments suggests that the lymphocyte has a developmental stage during which it possesses multiple heavy chains. Such observations have also been reported in several other studies (Creaves and Hogg, 1971;Bankhurst and Warner, 1971;Biozzi, Binaghi, Stiffel and Mouton, 1969;Sell, Lowe and Gell, 1970;Wilson, 1971). If one assumes that the superaddition phenomenon is due solely to lymphocytes bearing all three immunoglobulin classes on their surface, then there would still be many lymphocytes bearing only a single heavy chain type.…”

“…Such observations have also been reported in several other studies (Creaves and Hogg, 1971;Bankhurst and Warner, 1971;Biozzi, Binaghi, Stiffel and Mouton, 1969;Sell, Lowe and Gell, 1970;Wilson, 1971). If one assumes that the superaddition phenomenon is due solely to lymphocytes bearing all three immunoglobulin classes on their surface, then there would still be many lymphocytes bearing only a single heavy chain type.…”

Quantitative Features of a Sandwich Radioimmunolabeling Technique for Lymphocyte Surface Receptors

“…Direct study of such receptors in the mouse by means of antiglobulin reagents labeled with either fuorescent (4,5) or radioisotope (3,(6)(7)(8) markers has revealed a dense coating of surface immunoglobulins on nonthymus-derived or B lymphocytes 1 (5,8), but the question of receptors on the surface of thymus-derived or T lymphocytes remains more controversial. On the one hand, immunofluorescent (5) and some radioimmunolabeling (6) techniques have failed to show immunoglobulin on the surface of T lymphocytes, On the other hand, treatment of T lymphocytes with specific rabbit anti-mouse globulin reagents has inhibited lymphocyte-antigen interactions such as rosette formation (9,10) and radioactive antigen suicide (11). Moreover, in some (12)(13)(14) but not all (15) reports, in vitro binding of antiglobulin onto T lymphocytes has inhibited their capacity to mediate graft-vs.-host activity (12,13), delayed hypersensitivity responses (12), or T cell dependent helper functions (14).…”

The Effect of Cholesterol Oleate Treatment of Mice on the Rosette Forming Cell Response Against Sheep Erythrocytes

“…Another approach to the study of the cellular immune response is to use an immuno-cytoadherence technique such as that pioneered by Nota, LiacopoulosBriot, Stiffel and Biozzi [1964]. The precise significance of spontaneous and immune RFC in terms of their T-and B-lymphocyte content may be debatable, but the current evidence [Wilson, 1971a[Wilson, ,b, 1973Wilson and Miller, 1971; Elliott, Haskill and Axelrad, 1974;Warner, 1974] indicates that the population of lymphocytes forming rosettes is essentially different from that forming haemolytic plaques. The rosette forming cells (RFC) identified by this particular technique do not include a significant proportion of macrophages [Pavlovsky, Binet, Decreusefond, Stiffel, Bouthillier and Biozzi, 1970].…”