Blood and lens lipid peroxidation and antioxidant status in normal individuals, senile and diabetic cataractous patients

Donma, Orkide; Yorulmaz, Eda; Pekel, Hamiyet; Suyugül, Nezir

doi:10.1076/ceyr.25.1.9.9960

Cited by 68 publications

(57 citation statements)

References 34 publications

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“…Common biomarkers include antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, glutathione reductase, and catalase. A decrease in activities of these enzymes in both blood and lens samples in subjects suffering from cataract has been reported (Donma et al, 2002;Ozmen et al, 2002;Ates et al, 2004;Hashim et al, 2006). Paraoxonase-1 (PON1) is an enzyme with paraoxonase, aryl esterase, and diazoxonase activities (Canales and Sanchez-Muniz, 2003).…”

Section: Introductionmentioning

confidence: 88%

Comparative efficacy of captopril and allylmercaptocaptopril in the maintenance of oxidative-antioxidative equilibrium and attenuation of selenite-induced cataract in rats

Lakshmi¹,

Sharma²

2011

International Journal of Biological and Chemical Sciences

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The present study was undertaken to investigate the comparative efficacy of captopril (CP) and allylmercaptocaptopril (AMC) supplementation following selenite exposure with respect to oxidant-antioxidant equilibrium and prevents cataract formation in rat pups. Forty Wistar-albino rat pups were equally divided into 4 groups. In Group I; only subcutaneous (s.c) saline was injected. In Group II; single dose of sodium selenite (19 µM/kg body weight, s.c) was injected on postpartum day 10. One day before the selenite challenge, the pups in Group III and IV were injected intraperitoneally a single dose of 50 mg/kg of CP and AMC respectively and repeated once daily thereafter up to 21 st day. The development of cataract was assessed weekly. The density of cataract was graded by slit-lamp biomicroscopy. On day 21, the blood was collected and lenses were removed. Total oxidant status (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI) and malondialdehyde (MDA) level were determined in the lenses of the rat pups. Serum paraoxonase-1 (PON1) activity was determined. All of the lenses of rat pups in Group I remained clean. All rat pups developed dense nuclear opacity in Group II. Three out of 10 rat pups developed slight nuclear opacity in Group III while only one rat pup out of 10 in Group IV had vacuolization but not opacity. Differences among the groups were statistically significant (p<0.01). Group II lenses had higher mean OSI and MDA level than those of Group III and IV (p<0.01). Group II lenses had lower mean TAC level and serum PON1 activity than those of Group III and IV (p<0.01). A negative correlation was found between serum PON1 activity and lens MDA level (r = -0.6861; P<0.001). CP and AMC both diminished the incidence of cataract due to its protection of the antioxidant defense system but AMC was found to be more effective than CP.

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Section: Introductionmentioning

confidence: 88%

Comparative efficacy of captopril and allylmercaptocaptopril in the maintenance of oxidative-antioxidative equilibrium and attenuation of selenite-induced cataract in rats

Lakshmi¹,

Sharma²

2011

International Journal of Biological and Chemical Sciences

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“…Previous case-control studies were, however, inconclusive about the enrollment of systemic oxidative stress (6,10), and the possible reasons of the disturbed oxidant/antioxidant balance in SC (5,7,8,9,11). It has to be noted that the genesis of SC is of multiple origin, and some etiological risk factors, like visible and UV light exposure, have long-lasting but mostly local effects.…”

Section: Discussionmentioning

confidence: 99%

“…The resulting light scattering and lens opacification generally resemble the changes seen in human SC (2). Some other oxidants like LPO adducts, including organic hydroperoxides and ma londialdehyde (MDA), also have significant cataractogenic potential (1), and although they can be pre sent in systemic circulation at micromolar le vels and act as toxic messengers, the reports regarding the blood oxidative stress markers in patients with SC are largely inconsistent (5)(6)(7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

The Impact of Senile Cataract Maturity on Blood Oxidative Stress Markers and Glutathione-Dependent Antioxidants: Relations with Lens Variables

Mirić¹,

Kisic²,

Zoric³

et al. 2012

Journal of Medical Biochemistry

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The Impact of Senile Cataract Maturity on Blood Oxidative Stress Markers and Glutathione-Dependent Antioxidants: Relations with Lens VariablesOxidative stress is implicated in senile cataract (SC) genesis, although the impact of SC maturity on blood oxidative stress markers is unclear. Total hydroperoxides, malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST) were measured in the blood and lens samples of patients having either immature (n=31) or mature SC (n=50), and in 22 blood samples from noncataract controls. Compared to controls, SC patients had higher plasma MDA and serum GST, and decreased plasma GR and GSH levels. Plasma GPx as well as hydroperoxides differed from control values only in patients with mature SC. The multivariate logistic regression analysis showed that the fall of plasma GR activity (OR 5.14; CI 1.82-14.51;p=0.0020), as well as serum GST activity (OR 3.84; CI 1.36-10.83;p=0.0108) were independently associated with the maturity of SC. Lens hydroperoxides, MDA and GST, showed no correlation with correspondent blood values, in contrast to GPx (r=0.715; p<0.001) and GR (r=0.703; p<0.001). This study showed that the severity of SC is associated with increased systemic oxidative stress, which could be due to the fall of GSH-dependent antioxidant enzymes activities.

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“…15,16 So, many investigators used plasma or serum or erythrocyte to evaluate oxidant status in the patients of cataract and the normal controls. [15][16][17][18][19][20] One of the byproducts and index of lipid peroxidation is the toxic malondialdehyde (MDA), which is implicated in the development of the senile cataract manly due to its crosslinking ability. 21,22 Malondialdehyde (MDA) evidenced by the formation of thiobarbituric acid reactive substances (TBARS), was determined in serum.…”

Section: Introductionmentioning

confidence: 99%

Estimation of serum MDA (Malondialdehyde) in various morphological types and clinical stages of age related (senile cataract)

Angirekula

Atti

2018

Int J Adv Med

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Background: The aim was to estimate the Serum Malondialdehyde (MDA) in the various morphological types and clinical stages of Age Related (Senile) Cataract, a common cause of curable blindness, especially in India.Methods: This was a study of estimation of serum Malondialdehyde (MDA) in 100 patients of Age Related (Senile) cataract with 80 cases of Senile Cortical type (40 cases of Posterior subcapsular and 40 cases of Mature) and 20 cases of Senile Nuclear type (10 cases of early Grades of I and II and 10 cases of Advanced Grades of III and IV) and in 20 cases of normal controls in the age group of 45 to 70 years.Results: Serum MDA (nmol/ml) values of the Mean with SD were 3.34±0.93 (Range 2.02-4.65) in normal controls, 4.38±1.01 (Range 2.4 - 6.7) in senile cataract, 4.39±1.02 ((Range 2.4-6.7) in Senile Cortical Cataract with 3.82±0.73 (Range 2.4-5.1) in its Posterior Subcapsular stage and 4.96 ± 0.88 (Range 3.3-6.7) in its mature stage and 4.3±0.99 (Range 2.4-6.2) in Senile Nuclear cataract with 3.68±0.66 (Range 2.4-4.7) in its early Grades of I and II and 4.86±0.94 (Range 3.8-6.2) in its Advanced Grades of III and IV.Conclusions: The present study showed a significant increased concentration of Serum MDA in Senile cataract especially in the advanced later clinical stages or grades of maturity than in the earlier clinical stages or grades of immaturity of senile cortical or nuclear compared to normal controls. And there was no statistically significant difference between the morphological types of Age related (Senile) cataract i.e. Cortical Cataract compared to Nuclear Cataract.

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Blood and lens lipid peroxidation and antioxidant status in normal individuals, senile and diabetic cataractous patients

Abstract: A strong correlation was found between lens GSH and lens TBARS concentrations in the diabetic group. This emphasized the vital role of GSH as an antioxidant in the lens over the other antioxidant parameters, e.g., enzymes, and the oxidative stress is at the highest level in lens.

Cited by 68 publications

References 34 publications

Comparative efficacy of captopril and allylmercaptocaptopril in the maintenance of oxidative-antioxidative equilibrium and attenuation of selenite-induced cataract in rats

Comparative efficacy of captopril and allylmercaptocaptopril in the maintenance of oxidative-antioxidative equilibrium and attenuation of selenite-induced cataract in rats

The Impact of Senile Cataract Maturity on Blood Oxidative Stress Markers and Glutathione-Dependent Antioxidants: Relations with Lens Variables

Estimation of serum MDA (Malondialdehyde) in various morphological types and clinical stages of age related (senile cataract)

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