Blood B and T Lymphocytes and In Vitro Cellular Immune Reactivity in Untreated Human Malignant Lymphomas and Other Malignant Tumors

Eighty‐three non‐Hodgkin lymphomas classified according to the Kiel classification have been studied with regard to surface immunoglobulin (slg) on cells in suspension and cytoplasmic immunoglobulin (clg) by the peroxidase anti‐peroxidase method (PAP) on formaline‐fixed tissue sections. Fifty‐six out of 66 examined (i.e. 85%) revealed a monoclonal staining pattern for slg, whereas 37/70 (53%) gave a monoclonal staining pattern for clg by PAP. The methods combined gave a monoclonal staining pattern in 73/83, i.e. in 88%, of the biopsies tested. The discrepancies between the two methods were largest in centroblastic/centrocytic and lymphocytic lymphomas. With regard to the light chain staining patterns, complete agreement between the two methods was obtained in the 20 cases that allowed such analysis to be made. This suggests that the specificity of PAP, as carried out in this study with reagents purified by immunoabsorbent techniques, is satisfactory. On a basis of heavy chain isotypes centroblastic/centrocytic, lymphoplasmacytoid, and immunoblastic lymphomas could be divided into distinct immunological subgroups. In four biopsies the slg heavy chains were μ+δ, whereas μ+γ chains were detected by PAP. This finding may be relevant to the μγ switch known to occur during normal B‐cell differentiation. Immunoglobulin inclusions were found in 8 cases ‐ 3 belonging to the immunoblastic group, and 5 to the lymphoplasmacytoid group.

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“…The sheep red blood rosette test (E-rosettes) for detection of T-cells was carried out as described by Heier et al (8).…”

Section: E-rosettesmentioning

confidence: 99%

Cell‐associated Immunoglobulin in Human Non‐hodgkin Lymphomas

Landaas¹,

Godal²,

Marton³

et al. 1981

Acta Pathologica Microbiologica Scandinavica Section A Patholog

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Initiation of the blastogenic response of lymphocytes by hyperoptimal concentrations of concanavalin A

Steen

Lindmo

1979

Eur J Immunol

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The blastogenic response of human lymphocytes in vitro to hyperoptimal concentrations of concanavalin A (Con A) has been studied by means of volume spectroscopy (measuring cellular and nuclear volume), flow cytofluorometry (measuring cellular DNA content) and incorporation of [3H]thymidine ([3H]dThd). The optimal Con A dose with respect to [3H]dThd incorporation was about 30 micrograms/ml. In cultures given hyperoptimal doses, e.g. 100 micrograms/ml, [3H]dThd incorporation was strongly inhibited, whereas the number of cells entering S-phase and significantly increasing their cellular and nuclear volume was considerably larger than with 30 micrograms/ml. With 200 micrograms/ml Con A, which induced negligible [3H]dThd incorporation, the percentage of responding cells was even larger. Hence, doses of Con A, which were hyperoptimal with regard to [3H]dThd incorporation, induced blastogenic response, including DNA synthesis, in a larger percentage of the cells than did the optimal dose. However, in cultures with hyperoptimal Con A doses, the progression of the cell cycle stagnated mainly during S- and G2-phase and few cells completed mitosis. Thus, the blocking effect of hyperoptimal doses was not confined to any particular point of the cell cycle. The reduced [3H]dTd incorporation, seen with hyperoptimal doses, is attributed partly to a failure of this assay under such conditions.

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Altered membrane‐associated functions in chronic lymphocytic leukemia cells

Godal¹,

Henriksen²,

Ølandaas³

et al. 1978

Intl Journal of Cancer

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Peripheral blood lymphocytes consisting mainly of neoplastic B cells from patients with chronic lymphocytic leukemia (CLL cells) showed a markedly reduced response to the human B-cell mitogens anti-beta2 microglobulin, Sepharose-bound protein A and Sepharose-bound anti-human immunoglobulin (anti F(ab')2) in all of nine patients studied. On the other hand, CLL cells from three out of eight patients tested responded well to the calcium ionophore A23187. Sepharose-bound protein A and anti-beta2 microglobulin also failed to induce increased uptake of 86Rubidium (potassium analogue) in CLL cells as compared to B-cell-enriched preparations of normal peripheral blood lymphocytes. The capacity of CLL cells to cap various surface markers including beta2 microglobulin was reduced. On the other hand, surface concentrations of beta2 microglobulin were not reduced as measured by fluorescein-labelled anti-beta2-microglobulin in single-cell cytofluorometry. It is concluded that various membrane-associated events elicited by ligand-receptor interactions are altered or blocked in CLL cells.

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Blood B and T Lymphocytes and In Vitro Cellular Immune Reactivity in Untreated Human Malignant Lymphomas and Other Malignant Tumors

Cited by 15 publications

References 23 publications

Cell‐associated Immunoglobulin in Human Non‐hodgkin Lymphomas

Cell‐associated Immunoglobulin in Human Non‐hodgkin Lymphomas

Initiation of the blastogenic response of lymphocytes by hyperoptimal concentrations of concanavalin A

Altered membrane‐associated functions in chronic lymphocytic leukemia cells

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