Blood coagulation and anticoagulant mechanisms in the turtle Pseudemys elegans

Jacques, Felix A.

doi:10.1016/0010-406x(63)90047-1

Cited by 12 publications

(4 citation statements)

References 11 publications

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“…Additionally, cold temperatures can result in aberrant precipitation of blood plasma proteins such as fibrin (cryofibrinogenemia), leading to increased rates of clot formation and resulting in tissue ischaemia (Amdo & Welker, ). In association with these temperature‐induced complications, several winter hibernating species (both endothermic and ectothermic) display modifications to coagulation cascade which decrease risk of clotting and facilitate microcirculation in face of diminished circulatory performance (Cullinan, ; Jacques, ; Srere, Belke, Wang, & Martin, ).…”

Section: Discussionmentioning

confidence: 99%

Physiological and regulatory underpinnings of geographic variation in reptilian cold tolerance across a latitudinal cline

et al. 2018

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Understanding the mechanisms that produce variation in thermal performance is a key component to investigating climatic effects on evolution and adaptation. However, disentangling the effects of local adaptation and phenotypic plasticity in shaping patterns of geographic variation in natural populations can prove challenging. Additionally, the physiological mechanisms that cause organismal dysfunction at extreme temperatures are still largely under debate. Using the green anole, Anolis carolinensis, we integrate measures of cold tolerance (CT ), standard metabolic rate, heart size, blood lactate concentration and RNAseq data from liver tissue to investigate geographic variation in cold tolerance and its underlying mechanisms along a latitudinal cline. We found significant effects of thermal acclimation and latitude of origin on variation in cold tolerance. Increased cold tolerance correlates with decreased rates of oxygen consumption and blood lactate concentration (a proxy for oxygen limitation), suggesting elevated performance is associated with improved oxygen economy during cold exposure. Consistent with these results, co-expression modules associated with blood lactate concentration are enriched for functions associated with blood circulation, coagulation and clotting. Expression of these modules correlates with thermal acclimation and latitude of origin. Our findings support the oxygen and capacity-limited thermal tolerance hypothesis as a potential contributor to variation in reptilian cold tolerance. Moreover, differences in gene expression suggest regulation of the blood coagulation cascade may play an important role in reptilian cold tolerance and may be the target of natural selection in populations inhabiting colder environments.

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Section: Discussionmentioning

confidence: 99%

Physiological and regulatory underpinnings of geographic variation in reptilian cold tolerance across a latitudinal cline

et al. 2018

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“…Studies regarding haemostasis in reptiles (Belamarich and Eskridge, 1963; Jacques, 1963; Lewis, 1964, 1996; Zain‐ul‐Abedin and Katorski, 1966; Erdös et al., 1967; Hackett and Hann, 1967; Nahas et al., 1973, 1981; Denson, 1976; Lavras et al., 1979; Gorzula and Arocha‐Piñango, 1981; Arocha‐Piñango et al., 1982; Ratnoff, 1987; Ratnoff et al., 1990; Johnson and Benson, 1996) have rarely taken lizards, and especially the green iguana ( Iguana iguana ), into account. Moreover, the references often lack a detailed description of the methods, resulting in a limited meaningfulness of the results.…”

Section: Introductionmentioning

confidence: 99%

“…It cannot be excluded, either, that the existence of a natural inhibitor in iguana plasma could be responsible for this observation. Hints regarding inhibitors of which the coagulative effect is not yet known, exist in all reptilian orders (Jacques, 1963;Hackett and Hann, 1967;Nahas et al, 1973;Denson, 1976;Arocha-Pin˜ango et al, 1982). Because of the long in vitro clotting times, an influence of antithrombin has to be considered.…”

mentioning

confidence: 99%

Investigations on Blood Coagulation in the Green Iguana (Iguana iguana)

Kubalek

Mischke

Fehr

2002

Journal of Veterinary Medicine Series A

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The prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time, kaolin clotting time (KCT), dilute Russell's viper venom time (DRVVT) and reptilase time, as well as five different plasma fibrinogen assays [gravimetry, Jacobsson method (extinction at 280 nm), Millar method (heat precipitation), kinetic turbidometry, Clauss method] and resonance thrombography were performed in 26 clinically healthy green iguanas. All assays were carried out in comparison with pooled normal canine plasma. In iguana plasma, the PT [median (x0.50) = 453-831 s, dependent on the reagent], APTT (x0.50 = 170-242 s, dependent on the reagent), thrombin time (x0.50 = 118 - > 1000 s, dependent on thrombin activity), KCT (x0.50 = 274 s), DRVVT (x0.50 = 349 s) and reptilase time (all samples > 1000 s) were widely scattered at the limit of measurability. Only fibrinogen concentrations measured using the Jacobsson method (x0.50 = 4.40 g/l) correlated well (r = 0.91) with gravimetry (x0.50 = 4.22 g/l). The results of this study indicate a limited suitability and a confined diagnostic significance of the selected methods in the green iguana. This may be caused by the species specificity of certain components of the reagents used, as well as a less optimal test system, i.e. relationship of test reagent to clotting factor concentrations in iguana plasma.

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“…Os anticoagulantes naturais parecem estar amplamente distribuídos entre as ordens de répteis (JACQUES, 1963;DENSON, 1976;AROCHA-PIÑANGO, 1982). HACKETT E HANN (1967) sugeriram que a lentidão da coagulação dos répteis é devida, principalmente, à antitrombina circulante, sendo que essa antitrombina não é, comparativamente, mais potente que a de mamíferos, mas sua atividade se torna mais evidente pela deficiência da via intrínseca, que faz com que a conversão da protrombina em trombina seja mais lenta, tornando o efeito antitrombina mais proeminente.…”

Section: Hemostasia Comparadaunclassified

Purificação e caracterização da antitrombina do plasma da serpente Bothrops jararaca (Wied, 1824) (Ophidia: Viperidae, Crotalinae)

Morais¹

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Blood coagulation and anticoagulant mechanisms in the turtle Pseudemys elegans

Cited by 12 publications

References 11 publications

Physiological and regulatory underpinnings of geographic variation in reptilian cold tolerance across a latitudinal cline

Physiological and regulatory underpinnings of geographic variation in reptilian cold tolerance across a latitudinal cline

Investigations on Blood Coagulation in the Green Iguana (Iguana iguana)

Purificação e caracterização da antitrombina do plasma da serpente Bothrops jararaca (Wied, 1824) (Ophidia: Viperidae, Crotalinae)

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