Blood-Compatible Poly(2-methoxyethyl acrylate) Induces Blebbing-like Phenomenon and Promotes Viability of Tumor Cells in Serum-Free Medium

Abstract

Circulating tumor cells (CTCs) are highly related to tumor metastasis and an effective technique of detecting/isolating CTCs has been expected to be established for tumor treatments. Previously, we reported that platelets cannot adhere but tumor cells can adhere on poly(2-methoxyethyl acrylate) (PMEA) substrate. In this study, we report that cell viability of human fibrosarcoma (HT-1080) cells was promoted on PMEA substrate in serum-free medium. The significant blebbing-like phenomenon and spontaneous formation of cell aggregation were observed on PMEA substrate. Moreover, cell viability was promoted by activation of Akt signaling pathway via N-cadherin-mediated cell−cell contact. These results suggest that not only capture efficiency and purity but also high cell viability of CTCs can be accomplished by using PMEA substrate. PMEA substrate can be a promising candidate for medical applications such as in vitro screening of anticancer drugs and is an excellent platform for studies and diagnoses of tumor migration and metastasis.

Investigating the Intermediate Water Feature of Hydrated Titanium Containing Bioactive Glass

“…The base system showed a hump in the region of 25–35 2θ, BGT5 had its main hump in the range of 22–30 2θ, BGT7.5 exhibited a hump in the region of 10–25 2θ, and BGT10 showed a main hump in the range of 18–32 2θ. Same results have been reported for the inclusion of different transition metals in several studies; however, in most of these studies, no remarkable changes were observed in the amorphous nature of glass [ 12 , 13 , 14 ].…”

Modulation of Biological Responses of Tumor Cells Adhered to Poly(2-methoxyethyl acrylate) with Increasing Cell Viability under Serum-Free Conditions

“…Although it was unclear whether the blebbing types were phagocytotic, apoptotic, or migratory, the blebbing-like phenomenon of HT-1080 cells occurred on PMEA substrates under serum-free conditions, leading to an increase in cell viability . The interaction of cells and substrates is related to the factor to induce blebbing formation of cells. , To verify the effect of adhesion strength on the blebbing formation, we first investigated the differences in blebbing formation and cell morphology of cells adhered on PET, PBA, and PMEA.…”

“…The amoeboid movement of round cells is driven by actomyosin-based cortical contraction with blebbing formation, which is modulated by the activation of ROCK signaling. , ROCK inhibitors convert the movement and shape of cells from amoeboid (round shape) to mesenchymal (spread shape). Our previous report and the present study revealed that HT-1080 cells moved slowly by amoeboid movement with blebbing formation on PMEA substrates . To ascertain the effect of the ROCK pathway on the movement of cells, HT-1080 cells adhered to substrates were treated with Y-27632, a ROCK inhibitor (Figure f).…”

“…The culture of isolated CTCs obtained by label-free isolation can be used to allow the subsequent omics analyses of the tumor, such as transcriptomic, proteomic, metabolomic, and lipidomic analyses. − Although the maintenance of CTC viability and phenotypic and genotypic properties is difficult, CTC post-capture culture methods have been developed, including fluid flow, hypoxia, and three-dimensional cell culture. ,− However, it has also been reported that tumors and their stem cells cultured in serum-free media containing growth factors have phenotypes and genotypes closer to those of primary tumors than to those of serum-cultured tumor cells . With this in mind, we have previously developed a methodology to culture isolated CTCs using PMEA substrates in serum-free media . Tumor cells could adhere to PMEA substrates via not only integrin-dependent mechanisms but also integrin-independent mechanisms in the presence of serum, leading to the cell adhesion via the direct interaction of PMEA with cells under serum-free conditions .…”

“…Hence, PMEA substrates could collect tumor cells in a label-free manner using the integrin-independent mechanism under serum-free conditions. Furthermore, tumor cells adhered to PMEA-coated substrates showed higher viability under serum-free conditions than those on uncoated substrates . As one of the mechanisms, the blebbing-like phenomena of tumor cells on PMEA under serum-free conditions might be related to the increase in viability.…”