Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery

Kruse, Robert L.; Essén‐Gustavsson, Birgitta; Fossum, Caroline; Jensen-Waern, Marianne

doi:10.1186/1751-0147-50-32

Cited by 37 publications

(35 citation statements)

References 42 publications

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“…Levels of IL-10 also reflect the severity of Salmonella infection in mice [49]. In contrast, increased levels of IL-10 in blood coincided with recovery from experimentally induced swine dysentery [50]. In this study, IL-10 was not found in any intestinal sample.…”

Section: Discussioncontrasting

confidence: 52%

Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine

Šplı́chalová

Trebichavský

Rada

et al. 2010

Clinical and Experimental Immunology

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Section: Discussioncontrasting

confidence: 52%

Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine

Šplı́chalová

Trebichavský

Rada

et al. 2010

Clinical and Experimental Immunology

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“…In order to determine how factors from the immune system change in the pig colon during B. hyodysenteriae infection, we quantified the mRNA levels of 13 factors from the immune system, IL-1␤, IL-2, IL-4, IL-5, IL-6, IL-8, IL-12, IL-13, IL-17A, tumor necrosis alpha (TNF-␣), gamma interferon (IFN-␥), monocyte chemoattractant protein 1 (MCP-1), and neutrophil elastase (NE), by quantitative PCR (qPCR). These factors were chosen based on previous reports from experimental trials (13), associations with mucin production (5,(14)(15)(16), and/or in relation to histopathological findings, such as the neutrophilic infiltration observed in infected pigs (12). The colons of B. hyodysenteriae-infected pigs had a 7-fold increase in mRNA levels of the proinflammatory cytokine IL-17A compared to healthy controls (P ϭ 0.017) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…response in the pig colon during SD, and only systemic levels of several proinflammatory cytokines, such as IL-1␤, TNF-␣, and IL-6, have been reported for pigs after experimental inoculation with B. hyodysenteriae (13). From our observations in the colon tissue of B. hyodysenteriae-infected pigs, we determined a predominant increase in the mRNA levels of IL-17A, followed by increases in IL-1␤, IL-6, and IL-8 gene expression compared to the control pigs.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil Elastase and Interleukin 17 Expressed in the Pig Colon during Brachyspira hyodysenteriae Infection Synergistically with the Pathogen Induce Increased Mucus Transport Speed and Production via Mitogen-Activated Protein Kinase 3

et al. 2017

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Brachyspira hyodysenteriae colonizes the pig colon, resulting in mucoid hemorrhagic diarrhea and mucus layer changes. These changes are characterized by a disorganized mucus structure and massive mucus induction with de novo expression of MUC5AC and increased production of MUC2. To investigate the mechanisms behind this altered mucin environment, we quantified the mRNA levels of mucin pathway genes and factors from the immune system in the colons of infected and control pigs and observed upregulation of neutrophil elastase, SPDEF, FOXA3, MAPK3/ ERK1, In vitro, colonic mucus-producing mucosal surfaces were treated with these factors along with B. hyodysenteriae infection and analyzed for their effect on mucin production. Neutrophil elastase and infection synergistically induced mucus production and transport speed, and interleukin 17A (IL-17A) also had similar effects, in both the presence and absence of infection. A mitogen-activated protein kinase 3 (MAPK3)/extracellular signal-regulated kinase 1 (ERK1) inhibitor suppressed these effects. Therefore, we suggest that the SPDEF, FOXA3, and MAPK3/ERK1 signaling pathways are behind the transcriptional program regulating mucin biosynthesis in the colon during B. hyodysenteriae infection. In addition to furthering the knowledge on this economically important disease, this mechanism may be useful for the development of therapies aimed at conditions where enhancing mucus production may be beneficial, such as chronic inflammatory disorders of the colon.KEYWORDS cytokine, goblet cell, mucus, spirochete, swine T he mucus layer that lines the gastrointestinal tract serves as an important barrier against bacterial pathogens (1). The main components of the mucus layer are heavily glycosylated mucins, which are secreted by goblet cells (1). The mucin composition of the mucus layer varies along the gut, with a predominance of the secreted gel-forming mucins MUC5AC and MUC6 in the stomach and MUC2 in the small and large intestines (1). Penetration of the mucus layer and colonization of the epithelial surface by bacterial pathogens are decisive events for disease outcome. Mucins can have a protective role during infections by binding to bacteria and preventing further

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“…IL-10 down-regulates the production of pro-inflammatory cytokines and generally protects the animal from systemic inflammation [13]. The stimulatory effect of IL-10 on B cells can enhance antibody production and induce Ig-class switching and plasma cell differentiation [14]. Increased amounts of IL-10 inhibit the action of monocytes, macrophages, and NK cells during the immune response to viral infection and inhibit the synthesis of proinflammatory cytokines [12].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study for Cytokines and Immunoglobulin G in Swine

Lü

Liu

et al. 2013

PLoS ONE

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Increased disease resistance through improved immune capacity would be beneficial for the welfare and productivity of farm animals. To identify genomic regions responsible for immune capacity traits in swine, a genome-wide association study was conducted. In total, 675 pigs were included. At 21 days of age, all piglets were vaccinated with modified live classical swine fever vaccine. Blood samples were sampled when the piglets were 20 and 35 days of age, respectively. Four traits, including Interferon-gamma (IFN-γ) and Interleukin 10 (IL-10) levels, the ratio of IFN-γ to IL-10 and Immunoglobulin G (IgG) blocking percentage to CSFV in serum were measured. All the samples were genotyped for 62,163 single nucleotide polymorphisms (SNP) using the Illumina porcineSNP60k BeadChip. After quality control, 46,079 SNPs were selected for association tests based on a single-locus regression model. To tackle the issue of multiple testing, 10,000 permutations were performed to determine the chromosome-wise and genome-wise significance level. In total, 32 SNPs with chromosome-wise significance level (including 4 SNPs with genome-wise significance level) were identified. These SNPs account for 3.23% to 13.81% of the total phenotypic variance individually. For the four traits, the numbers of significant SNPs range from 5 to 15, which jointly account for 37.52%, 82.94%, 26.74% and 24.16% of the total phenotypic variance of IFN-γ, IL-10, IFN-γ/IL-10, and IgG, respectively. Several significant SNPs are located within the QTL regions reported in previous studies. Furthermore, several significant SNPs fall into the regions which harbour a number of known immunity-related genes. Results herein lay a preliminary foundation for further identifying the causal mutations affecting swine immune capacity in follow-up studies.

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Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery

Cited by 37 publications

References 42 publications

Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine

Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine

Neutrophil Elastase and Interleukin 17 Expressed in the Pig Colon during Brachyspira hyodysenteriae Infection Synergistically with the Pathogen Induce Increased Mucus Transport Speed and Production via Mitogen-Activated Protein Kinase 3

Genome-Wide Association Study for Cytokines and Immunoglobulin G in Swine

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