Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine

Šplı́chalová, Alla; Trebichavský, I; Rada, V.; Vlková, Eva; Sonnenborn, U.; Šplı́chal, Igor

doi:10.1111/j.1365-2249.2010.04283.x

Cited by 42 publications

(58 citation statements)

References 54 publications

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“…37 High IL-6 and IL-10 levels have been shown to predict greater mortality rates in septic patients. 38,39 Previous studies have shown that IL-6 -positive patients display significantly greater mortality rates than IL-6 -negative subjects. 40 IL-6, an early marker of sepsis, is applicable even in immunosuppressed patients.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Immunosuppression on Manifestations of Sepsis in an Animal Model of Cecal Ligation and Puncture

Kieslichova

Rocen

Merta

et al. 2013

Transplantation Proceedings

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Section: Discussionmentioning

confidence: 99%

The Effect of Immunosuppression on Manifestations of Sepsis in an Animal Model of Cecal Ligation and Puncture

Kieslichova

Rocen

Merta

et al. 2013

Transplantation Proceedings

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“…In a recent study, Splichalova et al [22] investigated the effect of giving Mutaflor or bifidobacteria before infecting gnotobiotic pigs with virulent Salmonella enterica . Pigs associated with bifidobacteria before Salmonella infection suffered from severe systemic infection and mounted similar cytokine responses as pigs infected with Salmonella alone.…”

Section: Basic Mechanisms Of Mutaflormentioning

confidence: 99%

Escherichia coli Nissle 1917 (Mutaflor): New Insights into an Old Probiotic Bacterium

Jacobi

Malfertheiner

2011

Dig Dis

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Probiotic bacteria are live microorganisms which contribute health benefits to their host. The numerous mechanisms of the probiotic-acting microorganisms include the induction of expression of certain cytokines and increasing the secretion of IgA and mucin. They activate lymphocytes and macrophages and inhibit the adhesion and invasion of epithelial cells. Escherichia coli Nissle 1917 (Mutaflor) is one of the most investigated probiotic bacteria. While the number of reports discussing the underlying mechanisms of Mutaflor has increased rapidly in recent years, novel clinical studies are missing. Here we provide an overview of the mechanisms of action and clinical studies related to Mutaflor. While most of the studies had positive outcomes, there are also a few in which Mutaflor did not perform to its expectations.

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“…When colonized together with EcN in Gn pigs, LGG fecal shedding and concentration in intestinal tissues trended toward lower than those of single colonization (Kandasamy et al, 2016), suggesting that the presence of EcN might inhibit the growth of LGG. Thus, it is likely that higher growth of EcN led to lower growth of LGG after RB feeding, and underlying mechanisms utilized by EcN need to be identified, such as competing for the nutrients and colonization sites, improving intestinal barrier, and modulating immune responses (Splichalova et al, 2011; Hering et al, 2014). In all, higher protective efficacy against HuNoV shedding and diarrhea might be achieved only if RB and LGG are given.…”

Section: Discussionmentioning

confidence: 99%

“…Gram-negative EcN is also a well-characterized probiotic used to treat diarrhea in infants and young children (Henker et al, 2007, 2008), as well as in neonatal large animals (von Buenau et al, 2005; Schroeder et al, 2006). The beneficial health effects are mediated via improving intestinal barrier function (Hering et al, 2014) or moderating inflammatory responses (Splichalova et al, 2011), which could protect Gn piglets from lethal infection of Salmonella Typhimurium (Splichalova et al, 2011). In addition, EcN was recently shown to have HRV-binding and immunomodulatory properties, resulting in significantly reduced HRV infection and diarrhea in Gn pigs (Kandasamy et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs

et al. 2016

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Probiotics have been recognized as vaccine adjuvants and therapeutic agents to treat acute gastroenteritis in children. We previously showed that rice bran (RB) reduced human rotavirus diarrhea in gnotobiotic pigs. Human noroviruses (HuNoVs) are the major pathogens causing non-bacterial acute gastroenteritis worldwide. In this study, Lactobacillus rhamnosus GG (LGG) and Escherichia coli Nissle 1917 (EcN) were first screened for their ability to bind HuNoV P particles and virions derived from clinical samples containing HuNoV genotype GII.3 and GII.4, then the effects of LGG+EcN and RB on HuNoV infection and diarrhea were investigated using the gnotobiotic pig model. While LGG+EcN colonization inhibited HuNoV shedding, probiotic cocktail regimens in which RB feeding started 7 days prior to or 1 day after viral inoculation in the LGG+EcN colonized gnotobiotic pigs exhibited high protection against HuNoV diarrhea and shedding, characterized by significantly reduced incidence (89 versus 20%) and shorter mean duration of diarrhea (2.2 versus 0.2 days), as well as shorter mean duration of virus shedding (3.2 versus 1.0 days). In both probiotic cocktail groups, the diarrhea reduction rates were 78% compared with the control group, and diarrhea severity was reduced as demonstrated by the significantly lower cumulative fecal scores. The high protective efficacy of the probiotic cocktail regimens was attributed to stimulation of IFN-γ+ T cell responses, increased production of intestinal IgA and IgG, and maintenance of healthy intestinal morphology (manifested as longer villi compared with the control group). Therefore, probiotic cocktail regimens containing LGG+EcN and RB may represent highly efficacious strategies to prevent and treat HuNoV gastroenteritis, and potentially other human enteric pathogens.

show abstract

Interference of Bifidobacterium choerinum or Escherichia coli Nissle 1917 with Salmonella Typhimurium in gnotobiotic piglets correlates with cytokine patterns in blood and intestine

Cited by 42 publications

References 54 publications

The Effect of Immunosuppression on Manifestations of Sepsis in an Animal Model of Cecal Ligation and Puncture

The Effect of Immunosuppression on Manifestations of Sepsis in an Animal Model of Cecal Ligation and Puncture

<i>Escherichia coli</i> Nissle 1917 (Mutaflor): New Insights into an Old Probiotic Bacterium

High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs

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