Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility

Weber, Marbod; Steinle, Heidrun; Golombek, Sonia; Hann, Ludmilla; Schlensak, Christian; Wendel, Hans Peter; Avci‐Adali, Meltem

doi:10.3389/fbioe.2018.00099

Cited by 483 publications

(342 citation statements)

References 111 publications

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“…Hemolytic activities for LATIS, PGA sutures, and silk sutures were 0.44%, 0.69%, and 0.60%, respectively, compared to fully hemolyzed positive control ( Figure B). Conventionally, blood‐contacting materials which result in less than 2% hemolysis are considered as nonhemolytic . In addition, exposure of LATIS and commercially available PGA and silk sutures to NIH 3T3 and BJ‐5Ta cell lines did not result in significant loss of cell viability at different time intervals (24, 48, and 72 h; Figure C).…”

Section: Resultsmentioning

confidence: 97%

Light‐Activated Tissue‐Integrating Sutures as Surgical Nanodevices

Ghosh

Urie

Chang

et al. 2019

Adv Healthcare Materials

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Sutures are typically the primary means of soft tissue repair in surgery and trauma. Despite their widespread use, sutures do not result in immediate sealing of approximated tissues, which can result in bacterial infection and leakage. Nonabsorbable sutures and staples can be traumatic to tissue, and the trauma can be exacerbated by their subsequent removal. Use of cyanoacrylate glues is limited because of their brittleness and toxicity. In this work, laser‐activated tissue‐integrating sutures (LATIS) are described as novel nanodevices for soft tissue approximation and repair. Incorporation of gold nanorods within fibers generated from collagen result in LATIS fibers which demonstrate robust photothermal responses following irradiation with near infrared laser light. Compared to conventional sutures, LATIS fibers result in greater biomechanical recovery of incised skin in a mouse model of skin closure after spine surgeries. Histopathology analyses show improved repair of the epidermal gap in skin, which indicate faster tissue recovery using LATIS. The studies indicate that LATIS‐facilitated approximation of skin in live mice synergizes the benefits of conventional suturing and laser‐activated tissue integration, resulting in new approaches for faster sealing, tissue repair, and healing.

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Section: Resultsmentioning

confidence: 97%

Light‐Activated Tissue‐Integrating Sutures as Surgical Nanodevices

Ghosh

Urie

Chang

et al. 2019

Adv Healthcare Materials

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“…Hemocompatibility is one of the major criteria for clinical success in blood-contacting biomaterials [16]. There are many haematological analysis models for analysing the haemocompatibility of biomaterials [20]. In this study, anticoagulated buffy coats with EDTA were used to find the mechanisms of the complement cascade on surface modified Ti coins.…”

Section: Discussionmentioning

confidence: 99%

“…This may reveal one of the weaknesses of this study, e.g., the detection and measurements of biomarkers. Weber et al suggested that these components could be bound to the surfaces [20] and it could be an indirect cause to the lack of differences for C3a and C5a on the various surfaces.…”

Section: Discussionmentioning

confidence: 99%

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Fluoride Modification of Titanium Surfaces Enhance Complement Activation

2020

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Immediately after dental implant insertion, blood will be in direct contact and interact with the implant surface and activates inflammatory responses and complement cascades within seconds. The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate complement cascades using the human buffy coat as model. The buffy coats were exposed to hydrofluoric acid-modified surfaces for a short time and its responses were compared to controls. Identification and quantification of complement cascade biomarkers were conducted using ELISA kits and multianalyte profiling using Luminex. A lower level of C3 at 30 min and increased levels of C4, MIP-4, CRP, and pigment epithelium-derived factor at 360 min were found on modified surfaces as compared to controls. We found no significant differences in the levels of C3a, C5a, C Factor H, α2M, ApoA1, ApoC3, ApoE, Prealbumin, α1AT, and SAP in modified surfaces in the buffy coats. We conclude that titanium surfaces treated with hydrofluoric acid modify the levels of specific biomarkers related to the complement cascade and angiogenesis and, thus, tissue growth, remodeling and repair, as this may play a role in the enhanced clinical performance of fluoride-modified Ti dental implants.

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“…The number of nonadherent cells (retention and consumption), their degree of activation as well as the formation of micro‐thrombi and platelet derived microparticles are classically determined . Expression of activation markers on the platelet membrane (e.g., P‐Selectin) and the release of the soluble agonists from granules and lysosomes (e.g., sP‐Selectin, Platelet Factor 4 and β‐thromboglobulin) indicate their degree of activation and can be studied via flow cytometry and ELISA . In many cases, dynamic in vitro test systems are applied, which allow repeated interactions between the circulating blood cells and the material surface (see section In vitro test systems).…”

Section: Characterization Of the Blood–materials Interactionsmentioning

confidence: 99%

In Vitro Thrombogenicity Testing of Biomaterials

Braune

Latour

Reinthaler

et al. 2019

Adv Healthcare Materials

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reliable, and reproducible. [20][21][22] This is, not least, important for realizing interstudy comparisons of the thrombogenicity of different materials. According, e.g., to the regulation (EU) 2017/745 of the European parliament and of the council on medical devices, in vitro and in vivo tests are a mandatory parts within the preclinical product verification and validation (see Annex II: Technical documentation, 6.1 Preclinical and clinical data). [23] Beyond the results of these tests, also detailed information concerning the test design, respective protocols and data analysis are obligatory to meet the requirements of the regulation, particularly those formulated in the ISO standard 10993 Part 4. [24,25] Since the regulatory agencies provide recommendations rather than protocols or standard operation procedures, accepted standardizations regarding the preanalytical handling of the blood, test conditions, test parameters, reference materials, static or dynamic conditions, flow conditions, and finally, which type of cells and donors should be included is lacking. Also, standard operating procedures how the testing should be performed are lacking. [9] This has led to a situation that laboratories perform in vitro assays under substantially different test conditions, including the preanalytical characterization of blood samples, the application of blood from different species, varying anticoagulation and stabilization or storage times as well as test setups. Taking these variations into account, interlaboratory or interstudy comparisons are impossible. [8,[26][27][28] Here, we review described test methods and procedures for the assessment of the thrombogenicity of materials, prerequisites for a reproducible in vitro testing, applied test system and test parameters for the characterization of the interaction of blood components/cells and the implant. We further review and outline recent approaches that may support realizing reliable, reproducible, and laboratory independent tests.

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Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility

Cited by 483 publications

References 111 publications

Light‐Activated Tissue‐Integrating Sutures as Surgical Nanodevices

Light‐Activated Tissue‐Integrating Sutures as Surgical Nanodevices

Fluoride Modification of Titanium Surfaces Enhance Complement Activation

In Vitro Thrombogenicity Testing of Biomaterials

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