Blood Flow Rate and Energy Charge in the Isolated Perfused Canine Liver

Rodrı́guez-Reynoso, Sergio; Portilla, Eliseo; García, David Suárez; Larios, F.; Romero, M.L.; Muñı́z, Jesús; Vázquez, Gumersindo Fernández; Leal, Caridad

doi:10.1006/jsre.2001.6102

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Cited by 3 publications

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“…Previously, some investigators reported normothermic extracorporeal liver perfusion systems [1,2], which were used as bioartificial livers for support in hepatic failure [3][4][5] or for preservation of liver grafts in transplantation [6,7]. However, these were not true artificial perfusion systems because their perfusates used organ blood as the oxygen carrier.…”

Section: Introductionmentioning

confidence: 98%

The Functional Integrity of a Normothermic Perfusion System Using Artificial Blood in Pig Liver

Nui

Kimura

Kikuchi

et al. 2006

Journal of Surgical Research

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Section: Introductionmentioning

confidence: 98%

The Functional Integrity of a Normothermic Perfusion System Using Artificial Blood in Pig Liver

Nui

Kimura

Kikuchi

et al. 2006

Journal of Surgical Research

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Successful Ex Vivo Normothermic Liver Perfusion with Purely Artificial Products Using Artificial Blood

Kimura¹,

Nui²,

Nagayama³

et al.

Artificial Oxygen Carrier

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Successful ex Vivo Normothermic Liver Perfusion with Purely Artificial Products using Artificial Blood

et al. 2003

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We tried to make an ex vivo functioning liver with an artificial perfusate that consisted of artificial blood in the pig liver. A liver graft from a female pig weighing 20 kg was harvested in the usual manner. The perfusion solution consisted of artificial blood, L-15 medium, distilled water, bovine serum albumin, NaHCO3, NaOH, KCl, human regular insulin, 50% glucose solution, and dexamethasone. The isolated liver was perfused with this oxygenated perfusate through the portal vein at a rate of 300 ml/min for 9 hours. Seven livers were perfused for 9 hours in this system. Five of the livers showed mean oxygen consumption of over 8 ml-02/min during perfusion. Histological findings showed that the hepatic architecture was almost completely preserved and numerous hepatocytes exhibited PAS-positive cytoplasmic glycogen deposits in these livers. These observations indicate that we have succeeded in developing an ex vivo functioning liver with an artificial perfusate employing artificial blood.

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Blood Flow Rate and Energy Charge in the Isolated Perfused Canine Liver

Cited by 3 publications

References 21 publications

The Functional Integrity of a Normothermic Perfusion System Using Artificial Blood in Pig Liver

The Functional Integrity of a Normothermic Perfusion System Using Artificial Blood in Pig Liver

Successful Ex Vivo Normothermic Liver Perfusion with Purely Artificial Products Using Artificial Blood

Successful ex Vivo Normothermic Liver Perfusion with Purely Artificial Products using Artificial Blood

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