Blood Glutathione as an Index of Radiation-Induced Oxidative Stress in Mice and Humans

Navarro, José; Obrador, Elena; Pellicer, José A.; Asensi, Miguel; Viña, José; Estrela, José M.

doi:10.1016/s0891-5849(96)00554-0

Cited by 158 publications

(96 citation statements)

References 24 publications

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“…This rise in peroxides indicated the occurrence of intensification of oxygen free radical production (26). Cells which are equipped with enzymatic antioxidant mechanisms play an important role in the elimination of free radicals.…”

Section: Discussionmentioning

confidence: 99%

Antitumor Activity and Antioxidant Status of the Methanol Extract of Careya arborea Bark Against Dalton’s Lymphoma Ascites-Induced Ascitic and Solid Tumor in Mice

et al. 2007

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Abstract. Based on the ethnomedical use of Careya arborea Roxb bark in the treatment of tumors, the present study was carried out to evaluate the anticancer potentials against Dalton's lymphoma ascites (DLA)-induced ascitic and solid tumors. The methanol extract of its bark given orally to mice at the dose of 250 or 500 mg / kg body weight for 10 days caused significant reduction in percent increase in body weight, packed cell volume, and viable tumor cell count when compared to the mice of the DLA control group. Restoration of hematological and biochemical parameters towards normal was also observed. Histological observations of liver and kidney also indicated repair of tissue damage caused by tumor inoculation. The extract at the dose of 5 or 25 mg / kg body weight given i.p. daily for 14 days significantly reduced the solid tumor volume induced by DLA cells.

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Section: Discussionmentioning

confidence: 99%

Antitumor Activity and Antioxidant Status of the Methanol Extract of Careya arborea Bark Against Dalton’s Lymphoma Ascites-Induced Ascitic and Solid Tumor in Mice

et al. 2007

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“…45 Cellular content of GSH and GSSG were measured by HPLC as previously described. 46,47 Determination of GSH in the extracellular medium…”

Section: Immunoprecipitation and Western-blot Analysismentioning

confidence: 99%

Aplidin® induces JNK-dependent apoptosis in human breast cancer cells via alteration of glutathione homeostasis, Rac1 GTPase activation, and MKP-1 phosphatase downregulation

et al. 2006

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Aplidins is an antitumor agent in phase II clinical trials that induces apoptosis through the sustained activation of Jun N-terminal kinase (JNK). We report that Aplidin s alters glutathione homeostasis increasing the ratio of oxidized to reduced forms (GSSG/GSH). Aplidin s generates reactive oxygen species and disrupts the mitochondrial membrane potential. Exogenous GSH inhibits these effects and also JNK activation and cell death. We found two mechanisms by which Aplidin s activates JNK: rapid activation of Rac1 small GTPase and downregulation of MKP-1 phosphatase. Rac1 activation was diminished by GSH and enhanced by L-buthionine (SR)-sulfoximine, which inhibits GSH synthesis. Downregulation of Rac1 by transfection of small interfering RNA (siRNA) duplexes or the use of a specific Rac1 inhibitor decreased Aplidin s -induced JNK activation and cytotoxicity. Our results show that Aplidin s induces apoptosis by increasing the GSSG/GSH ratio, a necessary step for induction of oxidative stress and sustained JNK activation through Rac1 activation and MKP-1 downregulation.

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“…It was observed that tumor cells produced more peroxides when they proliferate actively after inoculation of tumor. This rise in peroxides indicated the occurrence of intensification of oxygen free radical production [80]. Antioxidant molecules, such as GSH and vitamin E and C as well as antioxidant enzymes such as SOD, Catalase and glutathione peroxidase, have been long believed to have protective and anticancer activities by scavenging excess of free radicals.…”

Section: Discussionmentioning

confidence: 99%

In vivo antitumor potential of extracts from different parts of Bauhinia variegata linn. Against b16f10 melanoma tumour model in c57bl/6 mice

Pandey

2017

Appl Cancer Res

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Background: Melanoma is a metastatic type of skin cancer that is difficult to treat and the majority of efforts are directed to the design of new drugs. Medicinal Plants have been the primary source of medicines since life on earth; more than 50% of existing cancer treatments is derived from plants. Bauhinia variegata is well-known medicinal plant used from the ancient era to till date for their medicinal values. Scientific literatures have not documented any evidence of the antitumour potential of Bauhinia variegata against B16F10 melanoma tumor model in C57BL mice. The present investigation was undertaken to explore the antitumour activity of Leaf, stem bark and flower extract of Bauhinia variegata against B16F10 melanoma tumour model in C57BL mice. Methods: Hydro-methanolic extract prepared from the leaf, stem bark and flower of Bauhinia variegata were assessed for their antitumor activity. The extracts at doses of 500 and 750 mg/kg b.wt. were given orally along with cyclophosphamide (chemotherapeutic drug) for 40 days for exploring antitumor activity against melanoma tumor (B16F10) in C57BL mice. Inhibition of tumor growth, increase in survival time of animal with treatment, histopathological studies and antioxidant parameter were determined. Results: The Present investigation showed significant effect of the B. variegata L. in preventing melanoma tumor by B16F10 cell line in C57BL/6 mice. As compared with the tumour control group, the remarkable results especially in the group which received B. variegata extract and cyclophosphamide together were obtained for all of the measured parameters. Dose dependent response was observed in tumor volume, inhibition rate, life span time and antioxidant parameter of extracts. Combination treatment of cyclophosphamide and B. variegata extracts showed more pronounced effect. Conclusions: These findings suggest that B. variegata hydromethanolic extract may contain bioactive compounds of potential therapeutic significance which are relatively safe from toxic effects, and can compromise the medicinal use of this plant in folk medicine.

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Blood Glutathione as an Index of Radiation-Induced Oxidative Stress in Mice and Humans

Cited by 158 publications

References 24 publications

Antitumor Activity and Antioxidant Status of the Methanol Extract of Careya arborea Bark Against Dalton’s Lymphoma Ascites-Induced Ascitic and Solid Tumor in Mice

Antitumor Activity and Antioxidant Status of the Methanol Extract of Careya arborea Bark Against Dalton’s Lymphoma Ascites-Induced Ascitic and Solid Tumor in Mice

Aplidin® induces JNK-dependent apoptosis in human breast cancer cells via alteration of glutathione homeostasis, Rac1 GTPase activation, and MKP-1 phosphatase downregulation

In vivo antitumor potential of extracts from different parts of Bauhinia variegata linn. Against b16f10 melanoma tumour model in c57bl/6 mice

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