Blood glutathione redox ratio as a parameter of oxidative stress in premature infants with IRDS

Németh, Ilona; Boda, D

doi:10.1016/0891-5849(94)90036-1

Cited by 75 publications

(69 citation statements)

References 18 publications

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“…The laboratory methods used (briefly) were as follows. Glutathione assay: A highly sensitive and specific method was used (25). Twenty-five microliters of whole blood anticoagulated with EDTA was immediately hemolyzed in 2.5 mL of cold (4°C) 0.01 M potassium phosphate buffer containing 5 mM EDTA …”

Section: Methodsmentioning

confidence: 99%

Impaired Early Neurologic Outcome in Newborn Piglets Reoxygenated with 100% Oxygen Compared with Room Air after Pneumothorax-Induced Asphyxia

et al. 2001

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Section: Methodsmentioning

confidence: 99%

Impaired Early Neurologic Outcome in Newborn Piglets Reoxygenated with 100% Oxygen Compared with Room Air after Pneumothorax-Induced Asphyxia

et al. 2001

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“…Highly sensitive and specific separate determinations of GSSG and GSHϩGSSG concentrations were carried out by a previously published method [23]. Samples for GSSG measurement (25 l of whole blood collected over EDTA) were hemolyzed within 15 min after venipuncture with cold buffer in the presence of N-ethyl-…”

Section: Biochemical Analysis Of the Gsh And Hb Redox Systemsmentioning

confidence: 99%

Blood glutathione redox status in gestational hypertension

Németh

Orvos

Boda

2001

Free Radical Biology and Medicine

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Abstract-Gestational hypertension during the third trimester reflects an exaggerated maternal inflammatory response to pregnancy. We hypothesized that oxidative stress present even in normal pregnancy becomes uncompensated in hypertensive patients. A glucose-6-phosphate dehydrogenase (G6PD) activity sufficient to meet the increased reductive equivalent need of the cells is indispensable for defense against oxidative stress. The erythrocyte glutathione redox system was studied, where G6PD is the only NADPH source. The glutathione (GSH) redox status was measured both in vivo and after an in vitro oxidative challenge in pregnant women with gestational hypertension (n ϭ 19) vs. normotensive pregnant subjects (n ϭ 18) and controls (n ϭ 20). An erythrocyte GSH depletion with an increase in the oxidized form (GSSG) resulted in an elevated ratio GSSG/GSH (0.305 Ϯ 0.057; mean Ϯ SD) in hypertensive pregnant women vs. normotensive pregnant or control subjects (0.154 Ϯ 0.025; 0.168 Ϯ 0.073; p Ͻ .001). In hypertensive pregnant patients, a "GSH stability" decrease after an in vitro oxidative challenge suggested a reduced GSH recycling capacity resulting from an insufficient NADPH supply. The erythrocyte GSSG/GSH ratio may serve as an early and sensitive parameter of the oxidative imbalance and a relevant target for future clinical trials to control the effects of antioxidant treatment in women at increased risk of the pre-eclampsia syndrome.

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“…The rise in the level of TAC could be due to its induction to counter the effect of increased oxidative stress. A significant decrease in GSH/GSSG redox ratio was associated with a significant increase in the oxidized GSSG disulfide form of glutathione (Nemeth & Boda, 1994). This is due to the reaction of GSH with free radicals catalyzed by glutathione peroxidase generating the GSSG.…”

Section: Discussionmentioning

confidence: 99%

Tea and Mint Extracts Modulate the HSP70 Expression in Preeclamptic Placental Explant

Padmini¹

2012

J. App. Pharm. Sci.

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Heat shock protein 70 is a molecular chaperone which expressed during oxidative stress to protect the cell from damage. Preeclampsia is a hypertensive disorder with an oxidative stress imbalance. Antioxidant defenses appear to be depleted during preeclampsia resulting in increased oxidative stress. Various alternative medicines are employed to increase the endogenous antioxidant level during preeclampsia. The aim of the present study was to determine the modulatory effect of medicinal plants, Camellia sinensis and Mentha spicata on oxidative stress, antioxidant and thiol status using placental explant as the model system. Placental explants were cultured from the placental tissues of both normotensive and preeclamptic subjects. The lipid peroxide, total antioxidant capacity, glutathione redox ratio, HSP70 levels were measured in the placental explants with and without incubation with tea, mint and mint-tea. The addition of these three extracts increased the TAC and GRR in both placental explants with decrease in the LPO level. The expression of HSP70 also decreased more significantly in preeclamptic explants on addition of tea and mint extracts due to the restoration of cell homeostasis via maintaining the antioxidant status. In view of the above results, mint-tea may emerge as an effective antioxidant, preventing cell damage during stress condition.

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Blood glutathione redox ratio as a parameter of oxidative stress in premature infants with IRDS

Cited by 75 publications

References 18 publications

Impaired Early Neurologic Outcome in Newborn Piglets Reoxygenated with 100% Oxygen Compared with Room Air after Pneumothorax-Induced Asphyxia

Impaired Early Neurologic Outcome in Newborn Piglets Reoxygenated with 100% Oxygen Compared with Room Air after Pneumothorax-Induced Asphyxia

Blood glutathione redox status in gestational hypertension

Tea and Mint Extracts Modulate the HSP70 Expression in Preeclamptic Placental Explant

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