Blood group analysis from cigarette butts by absorption inhibition method: An experimental study

Ruth, Mieke Sylvia Margaretha Amiatun; Purnadianti, Melly; Marini, Maria Istiqomah

doi:10.4103/jioh.jioh_219_19

Cited by 2 publications

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“…The secretor status could be identified by two methods - the absorption-inhibition method (ab-in) and the absorption-elution method. Ruth et al conducted a study to determine blood groups from cigarette butts [ 10 ]. Velani et al conducted research on 47 dry salivary samples and showed that 100% of the subjects were secretors [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Salivary ABO Blood Group Antigens and Secretor Status in Sriganganagar, Rajasthan: A Correlational Analysis of 300 Samples

Rajawat¹,

Ramalingam²,

Pareek³

et al. 2023

Cureus

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To estimate the ABO blood groups from saliva samples and to correlate with the secretor status. Materials and methodsA sample size of 300 individuals was selected from the outpatient department of Surendera Dental College & Research Institute, Sriganganagar, India, and from dental camps organized by the college in the near vicinity. Informed consent was obtained from selected individuals to collect their blood and saliva samples. Salivary samples were evaluated for ABO blood groups by the absorption-inhibition method. The indicator erythrocytes were prepared after blood group confirmation from serum. It was used to identify the blood group antigens in saliva to confirm the secretor status. The results were tabulated and the Pearson's chisquared test was performed for statistical analysis using SPSS 15.0 (SPSS Inc., Chicago, IL). ResultsThe present study showed that 282 subjects (94%) were Rhesus positive and 18 subjects (6%) were Rhesus negative. Two-hundred-and-fifty subjects (83.3%) were secretors of antigens in saliva. Non-secretors were 50 subjects (16.7%). We identified that 250/300 were secretors and the majority were in AB & A group. ConclusionBlood groups could not be detected from the saliva of subjects who were non-secretors. In contrast, blood types could be accurately identified from the saliva of those subjects who were secretors of antigen.

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Section: Discussionmentioning

confidence: 99%

Assessment of Salivary ABO Blood Group Antigens and Secretor Status in Sriganganagar, Rajasthan: A Correlational Analysis of 300 Samples

Rajawat¹,

Ramalingam²,

Pareek³

et al. 2023

Cureus

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Prevalence of ABH(O) Blood Group Secretors Among Indigenous Population of Shillong: A Cross-Sectional Study

Das,

Slong,

Ropmay

et al. 2024

Cureus

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Background ABH antigens on the surface of red blood cells form Type A, B, AB, and O blood groups. These antigens are also found in body secretions like serum, gastric juices, ovarian cyst fluid, semen, amniotic fluid, sweat, urine, tears, bile, and saliva (except cerebrospinal fluid). People who have these antigens in their body fluids are called secretors. Purpose This study was conducted to find out the prevalence of salivary secretor status among the adult local indigenous population of Shillong, India. Method Unstimulated saliva was collected and processed from 250 apparently healthy adults, determined its secretor status using the absorption-inhibition method, and then analyzed using the Chi-square test to find its association with age, sex, tribe, and blood groups. Results The study found a prevalence of salivary secretor status of 57.2% (n=143), with a slight female predominance of 57.7% (n=64) over males (56.8%, n=79). The highest percentage was found in individuals aged 71-80 (68.8%, n=16), while the least in people aged 18-30 (48.2%, n=40). Khasi tribes had the highest incidence (64.2%, n=88), followed by Garo (55%, n=22), and least in Jaintia tribes (45.2%, n=33). O-blood group individuals had the highest percentage (71%, n=22), while AB-blood group individuals had the least (46%, n=23). We observed a statistically significant correlation of salivary secretor status with the blood group and tribe of the individuals but not with their sex and age. Conclusion Saliva can be found on various items in medicolegal cases like robberies, rapes, and hangings, but blood stains may not always be present. Saliva can be used to determine blood groups, potentially aiding in resolving claims of parentage, immigration, kidnapped children, disputed paternity, or identifying victims in mass disasters. ABO(H) determination from body fluids, especially saliva, has been used in forensic investigations before DNA technology. Currently, this can be an initial screening method because DNA methodology is not often available in laboratories in India.

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Blood group analysis from cigarette butts by absorption inhibition method: An experimental study

Cited by 2 publications

References 1 publication

Assessment of Salivary ABO Blood Group Antigens and Secretor Status in Sriganganagar, Rajasthan: A Correlational Analysis of 300 Samples

Assessment of Salivary ABO Blood Group Antigens and Secretor Status in Sriganganagar, Rajasthan: A Correlational Analysis of 300 Samples

Prevalence of ABH(O) Blood Group Secretors Among Indigenous Population of Shillong: A Cross-Sectional Study

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