Background: In saliva, neutrophil constitutes the most prominent first-line defense of immune cells against pathogenic microbes. The importance of neutrophils to the host immune systems of neutropenic or patients disabled with regard to their neutrophil function results in a tendency toward serious infections, such as early childhood caries (ECC). The cytoplasmic granules present in neutrophils play a major role in neutrophil-mediated inflammation. Azurophilic granules contain antimicrobial proteins, such as defensin, a human antimicrobial peptide (HNP 1–3). The aim of this study is to analyze the correlation of HNP 1–3 secretion with CD63 expression on the surface of salivary neutrophils. Materials and Methods: This study constituted a cross-sectional, analytical observational study. Saliva taken from preschoolchildren between the ages of 4–6 years who had been divided into two groups, i.e., early childhood caries group with decayed, extracted, filled teeth (def-t) index >6 and caries free with def-t = 0, was subjected to a HNP 1–3 secretion test using ELISA assay and an expression test for CD63 by means of a flow cytometry test. The results obtained were analyzed using independent t -test and Pearson correlation ( P < 0.05). Results: The secretion of HNP 1–3 in the saliva of ECC was higher (172.6 ± 41.64) compared to that of caries-free cases (140.39 ± 31.91), whereas the level of CD63 salivary expression in ECC was lower (2.32 ± 0.57) than in the presence of caries (2.67 ± 0.46). Conclusion: In ECC cases, saliva increases HNP 1–3 secretion but decreases CD63 expression on the surface of salivary neutrophils.

Background: Forensic dentistry identification commonly involves using dental cast models as ante-mortem data. Here, dentists generally send the pictures as well as the dental records. However, in recent times, dentists – especially orthodontists and prosthodontists – are using 3D scanners in view of reducing the space for cast model storage as well as sending the 3D imaging for fabricating clear aligners and other items such as crowns and bridges. This new trend means data transmission and viewing has become more complicated since sophisticated laptops or personal computers are generally required. For more practical use, smartphones would be a better option, meaning various simple ideas for viewing 3D data must be explored. Furthermore, the conclusions must be evaluated in terms of the validity for forensic dentistry use. Purpose: To evaluate a number of smartphone applications that are simple, user friendly, scalable and capable of the measurement and superimposition of 3D imaging data. Review: Standard tessellation language (STL) is one of the 3D scan file formats that is also useful for 3D printing. Recently, several applications for 3D viewing have been made available for iPhones (iOS) and Android-based devices, which are able to view STL files. However, they have all received both positive and negative reviews in terms of various applications, including forensic dentistry, and they thus require further evaluation by forensic odontologists. Conclusion: Each application has advantages and disadvantages; however, in our experience as forensic odontologists, the CAD Assistant, exocad and Adobe Photoshop Mix, which are available for iOS and Android devices, are preferable for forensic dentistry needs

Lip prints and bloodstains may be left behind in many crime scenes and may reveal the identity of the victim and the suspected person. This study was aimed to investigate the inheritance pattern of lip prints and blood groups among parents and their offspring in the Javanese population. 105 subjects from 25 Javanese family frames participated in this study. Lips print pattern was recorded and analyzed according to Suzuki and Tsuchihashi classification. A blood group test was also performed for each subject. The statistical analysis carried out using IBM® SPSS® Statistics version 23.0 (IBM, Armonk, New York, USA). The results of this study revealed that type II was the predominant pattern of lip prints among the Javanese population (34.3%). Blood group A was the predominant type in subjects with lip prints type II (15%). The result also shows that the lip prints pattern in girls tends to be inherited from the mother. However, the inheritance of lip prints pattern in boys couldn't be determined precisely. The heritability of lip prints pattern was observed between parents and their offspring. Also, there was a tendency of blood groups to have a certain pattern of lip prints.

Age estimation is one of the important components in forensic science used for personal identification, biological profile reconstruction, and help narrowing the search possibilities. Age estimation can be done by various methods and biological evidence, such as the human face. The human face is one of biometrics that provides a variety of information.  The purpose of this article is to evaluate the accuracy and reliability of age estimation with face using smartphone for forensic identification based on previous studies and experiences. Age estimation by face is based on age progression that causes attrition and degeneration on soft tissue. With the development of technology, age estimation by face can be done with applications or websites on smartphone. In general, the utilization of smartphone can reduce waste, pollution, research cost and easier to save and share. A lot of applications have been developed and free to download. Unfortunately, the accuracy of its results is unknown. In conclusion, the applications for age estimation on smartphone give quiet good results and can be used as a supporting tool to estimate age in forensic identification.

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