Blood Levels of the Prostaglandin F_2α Metabolite during the Postpartum Period in Bos indicus Cows in the Humid Tropics

Abstract

Summary Plasma levels of the prostaglandin F2α metabolite (15‐ketodihydroprostaglandin F2α) were determined by radioimmunoassay in 26 postpartum multiparous Brahman cows. Variation in the concentrations of the metabolite was high among animals. 15‐Ketodihydro‐PGF2α concentrations on day 0, 5, 10, 15 and 20 postpartum ranged from 402 to ≥1000, from 251 to ≥1000, from 237 to ≥1000, from 75 to 608 and from 75 to 267 pmol l−1, respectively. The prostaglandin metabolite levels were elevated at parturition and remained elevated thereafter for periods varying up to 10 to 23 days postpartum (16.7 ± 0.7 days, mean ± SEM). Uterine involution (UI) was completed by 28.0 ± 0.8 days (ranging from 20 to 36 days). A markedly linear decrease of 15‐ketodihydro‐PGF2α levels (P < 0.001) occurred from parturition until Day 23 postpartum. A significant correlation between the duration of the elevated PGF2α metabolite and the time required for completion of UI was observed (r = −0.50, P < 0.01). The effect of parity (PT) on 15‐ketodihydro‐PGF2α levels was not significant, however the relationship between UI and PT was affected (r = −0.59, P < 0.001). Both PGF2α release and PT had effects on the variability of UI (R2 = 0.62, P < 0.001). No significant relationship was found between elevated levels of 15‐ketodihydro‐PGF2α, UI and PT with postpartum anoestrous interval (PPAI).

Impact of bypass fat and mineral supplementation peripartum on plasma profile of steroid hormones PGFM and postpartum fertility in Jaffarabadi buffaloes

“…The observed trend of changes in PGFM values was found to be similar to the findings of Thompson et al (1987), Toribio et al (1994) and Dhami et al (2017), who all reported that the PGFM levels in the prepartum days were low, but increased many fold around parturition. The levels thereafter came back to normal within 20-30 days postpartum.…”

Comparación de dos protocolos para sincronizar ovulación e implementar inseminación artificial en vaquillonas

“…La determinación de P4 se realizó en la Facultad de Ciencias Veterinarias (UNCPBA, Tandil, Buenos Aires). Se utilizó un kit para RIA (COAT A COUNT, Siemens Healthcare Diagnostic Inc., CA, USA) previamente validado para bovinos 14 . El coeficiente de variación intra-ensayo fue < 7% para concentraciones entre 0,1 y 40 ng/ml y el inter-ensayo fue < 3,5%.…”

Effect of estradiol cypionate and amount of progesterone in the intravaginal device on synchronization of estrus, ovulation and on pregnancy rate in beef cows treated with FTAI based protocols

“…Plasma samples were assayed at the Facultad de Ciencias Veterinarias, U.N.C.P.B.A., Tandil, Argentina. Samples were analyzed using a progesterone RIA kit (COAT A COUNT, Siemens Healthcare Diagnostic Inc., CA, USA) previously validated for use with bovine plasma (Toribio et al, 1994). The intra-assay coefficient of variation was less than 7% for concentrations between 0.1 and 40 ng/mL, the interassay coefficient of variation was less than 3.5% and assay sensitivity was 0.01 ng/mL.…”