Blood microbiota composition in Iranian pre-diabetic and type 2 diabetic patients1

Ghaemi, Farahnaz; Fateh, Abolfazl; Sepahy, Abbas Akhavan; Zangeneh, Mehrangiz; Ghanei, Mostafa; Siadat, Seyed Davar

doi:10.3233/hab-210450

Cited by 5 publications

(5 citation statements)

References 38 publications

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“…Six of thirty-one studies focused on the T2D comprising of 4992 individuals. Out of six studies, four studies have a randomized controlled population [ 47 , 49 , 51 , 59 ] while one study has no controls [ 50 ]. Five studies used different variable regions and sequencing methods using the rRNA while Amar et al used rDNA [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

“…[ 46 ] which may increase one’s risk. Pre-diabetic and patients with morbid obesity tend to show lower abundance of Akkermansia , and Faecalibacterium ( Table 2 ) compared to healthy individuals [ 49 ]. However, Bacteroides was found in abundance in people who were statistically less likely to develop T2D [ 47 ], suggesting that Bacteroides may act as a buffer against the development of T2D.…”

Section: Resultsmentioning

confidence: 99%

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Blood Stream Microbiota Dysbiosis Establishing New Research Standards in Cardio-Metabolic Diseases, A Meta-Analysis Study

Goraya

et al. 2023

Microorganisms

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Aims: Scientists have recently discovered a link between the circulating microbiome and homeostasis, as well as the pathogenesis of a number of metabolic diseases. It has been demonstrated that low-grade chronic inflammation is one of the primary mechanisms that has long been implicated in the risk of cardio-metabolic disease (CMDs) and its progression. Currently, the dysbiosis of circulating bacteria is considered as a key regulator for chronic inflammation in CMDs, which is why we have conducted this systemic review focused on circulating bacterial dysbiosis. Methods: A systemic review of clinical and research-based studies was conducted via PubMed, Scopus, Medline, and Web of Science. Literature was considered for risk of bias and patterns of intervention effects. A randomized effect model was used to evaluate the dysbiosis of circulating microbiota and clinical outcomes. We conducted a meta-analysis considering the circulating bacteria in both healthy people and people with cardio-metabolic disorders, in reports published mainly from 2008 to 2022, according to the PRISMA guidelines. Results: We searched 627 studies and, after completing the risk of bias and selection, 31 studies comprising of 11,132 human samples were considered. This meta-analysis found that dysbiosis of phyla Proteobacteria, Firmicutes, and Bacteroidetes was associated with metabolic diseases. Conclusions: In most instances, metabolic diseases are linked to higher diversity and elevated bacterial DNA levels. Bacteroides abundance was higher in healthy people than with metabolic disorders. However, more rigorous studies are required to determine the role of bacterial dysbiosis in cardio-metabolic diseases. Understanding the relationship between dysbiosis and cardio-metabolic diseases, we can use the bacteria as therapeutics for the reversal of dysbiosis and targets for therapeutics use in cardio-metabolic diseases. In the future, circulating bacterial signatures can be used as biomarkers for the early detection of metabolic diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Blood Stream Microbiota Dysbiosis Establishing New Research Standards in Cardio-Metabolic Diseases, A Meta-Analysis Study

Goraya

et al. 2023

Microorganisms

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“…In the case of visceral tissues previously considered sterile, it has been repeatedly shown that they contain microbial genetic material ( Aagaard et al, 2014 ; Ghaemi et al., 2021 ; Sookoian and Pirola, 2021 ). Indeed, hereby we could detect these from healthy arterial wall.…”

Section: Discussionmentioning

confidence: 99%

Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors

Hidi

Kovács

Szabó

et al. 2022

Front. Cell. Infect. Microbiol.

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Discovery of human microbiota is fundamentally changing our perceptions of certain diseases and their treatments. However little is known about the human blood vessel microbiota, it may have important effects on vascular pathological lesions and vascular homograft failure. In our prospective survey study fourteen femoral arteries, harvested from donors in multi-organ donations, were examined using the V3-V4 region 16S rRNA sequencing method. The most abundant phyla in the human vascular microbiota were Proteobacteria, Firmicutes and Actinobacteria. At the genus level, the most abundant taxa were Staphylococcus, Corynebacterium, Pseudomonas, Bacillus, Acinetobacter and Propionibacterium. Of the bacterial taxa that have an indirect effect on the development of atherosclerosis, we found Porphyromonas gingivalis, Prevotella nigrescens and Enterobacteriaceae spp. with different abundances in our samples. Of the bacteria that are more common in the intestinal flora of healthy than of atherosclerosis patients, Roseburia and Ruminococcus occurred in the majority of samples. The human arterial wall has a unique microbiota that is significantly different in composition from that of other areas of the body. Our present study provides a basis for ensuing research that investigates the direct role of the microbiota in vascular wall abnormalities and the success of vascular allograft transplantations.

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“…Patients with pre-diabetes or type 2 diabetes have fewer Faecalibacterium , Akkermansia , and Bifidobacterium bacteria in their leukocytes than healthy people, according to an epidemiological study on Iranian diabetes patients. The bacterial loads of E. coli and Bacteroides fragilis were higher in pre-diabetic individuals than healthy individuals, while type 2 diabetes groups had higher Lactobacillus , E. coli , and Bacteroides fragilis ( Ghaemi et al., 2021 ). However, according to a case study in the Chinese population, no differences were reported in the baseline microbiota diversity of diabetes and control participants.…”

Section: Healthy Blood Bacteria Dysbiosis In Chronic Diseasesmentioning

confidence: 97%

Human circulating bacteria and dysbiosis in non-infectious diseases

Goraya

Mannan

et al. 2022

Front. Cell. Infect. Microbiol.

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Blood microorganisms were once thought to indicate infection. Blood in healthy people appears to be devoid of growing bacteria; nonetheless, intracellular dormant forms of bacteria have been reported previously. With breakthroughs in sequencing and bioinformatics, the presence of bacterial DNA in healthy human blood initiated the controversy of human blood microbiota (HBM). Recently, bacteria-specific DNA and culturable bacteria were found in healthy human blood. Researchers wanted to study the phenomena of a “healthy blood microbiota” by providing a thorough description of bacterially produced nucleic acids using many complementing molecular and traditional microbiological approaches. Because blood is a relatively limited and particular environment, culturability and plate count issues can be overcome using enhanced cultured procedures. However, more evidence is required to confirm that healthy human blood contains normal microbiota. Cavities, mouth and intestinal microbiota, trauma, surgery, and animal/insect bites can introduce bacteria into human blood. All these factors strengthen the concept of transient blood bacteria too. The presence of blood bacteria may be caused by temporary immunological clearance and absorption by dendritic or M cells. This review provides an extensive and comprehensive analysis that suggests that healthy blood bacteria may not be typical microbiota but transient circulatory microorganisms. In this study, we look at how contaminants (Escherichia, Shigella, Pseudomonads, etc.) from the skin, laboratory environments, and reagents can affect the interpretation of blood-derived microbial information and the relationship between the circulating bacteria and non-communicable diseases. Circulating transient bacteria may play a role in the pathogenesis of non-infectious diseases such as diabetes and CVD. Contamination-free hematological studies can aid in understanding the disease mechanisms, therapy, and biomarkers.

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Blood microbiota composition in Iranian pre-diabetic and type 2 diabetic patients1

Cited by 5 publications

References 38 publications

Blood Stream Microbiota Dysbiosis Establishing New Research Standards in Cardio-Metabolic Diseases, A Meta-Analysis Study

Blood Stream Microbiota Dysbiosis Establishing New Research Standards in Cardio-Metabolic Diseases, A Meta-Analysis Study

Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors

Human circulating bacteria and dysbiosis in non-infectious diseases

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