Blood Plasma Metabolome Profiling at Different Stages of Renal Cell Carcinoma

Abstract

Early diagnostics significantly improves the survival of patients with renal cell carcinoma (RCC), which is the prevailing type of adult kidney cancer. However, the absence of clinically obvious symptoms and effective screening strategies at the early stages result to disease progression and survival rate reducing. The study was focused on revealing of potential low molecular biomarkers for early-stage RCC. The untargeted direct injection mass spectrometry-based metabolite profiling of blood plasma samples from 51 non-cancer volunteers (control) and 78 patients with different RCC subtypes and stages (early stages of clear cell RCC (ccRCC), papillary RCC (pRCC), chromophobe RCC (chrRCC) and advanced stages of ccRCC) was performed. Comparative analysis of the blood plasma metabolites between the control and cancer groups provided the detection of metabolites associated with different tumor stages. The designed model based on the revealed metabolites demonstrated high diagnostic power and accuracy. Overall, using the metabolomics approach the study revealed the metabolites demonstrating a high value for design of plasma-based test to improve early ccRCC diagnosis.

Influence of Dietary Polyunsaturated Fatty Acid Intake on Potential Lipid Metabolite Diagnostic Markers in Renal Cell Carcinoma: A Case-Control Study

“…In this study, the entire amino acid pathway was found to be downregulated, aligning with previous findings [6,16,46]. RCC subtypes exhibited significant changes in amino acid metabolism and redox homeostasis [46].…”

“…To the best of our knowledge, 13 prior case-control studies have identified potential metabolic biomarkers in plasma [6][7][8][9][10] or serum [11][12][13][14][15][16][17][18] that distinguish RCC from controls. The proposed profiles include lipids, lipoproteins [6][7][8][9][13][14][15]17,18], amino acids [6][7][8]10,[12][13][14]17], and carbohydrates [7,11,13,14,16]. However, robust RCC diagnostic biomarkers remain unclear.…”

Causal associations between atrial fibrillation and breast cancer: A bidirectional Mendelian randomization analysis

“…Among the top causes of death worldwide, cardiovascular disease and cancer constitute about nearly half of global deaths. 1 Atrial fibrillation (AF) is the most prevalent sustained arrhythmia worldwide and a well‐known factor in cardiovascular morbimortality. 2 , 3 Besides, AF patients are also confronted with a substantial risk of mortality from non‐cardiovascular causes.…”

Therapeutic effects and underlying mechanism of poly (L-glutamic acid)-g-methoxy poly (ethylene glycol)/combretastatin A4/BLZ945 nanoparticles on Renca renal carcinoma

“…Surgical treatment is the preferred option for managing renal carcinoma, while immunotherapies have shown sensitivity instead of chemotherapy drugs ( Klapper et al, 2008 ; Aggen et al, 2021 ). Currently, the prognosis of targeted drugs that combined to inhibit angiogenesis is unsatisfactory ( Maslov et al, 2022 ; Li et al, 2023 ). Previous studies have shown that renal carcinoma is a malignant tumor that relies on angiogenesis and hypoxia.…”