Blood Platelets of the African Elephant

Plessis, Lizette du; Stevens, Kenneth R.

doi:10.1053/jcpa.2002.0565

Cited by 12 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies done include animals ranging from rhinoceros, elephant, and bovine species (du Plessis and Stevens, 2002;du Plessis et al 1996;Liu et al, 1998;Zucker-Franklin et al, 1985). Chart 1.…”

Section: Introductionmentioning

confidence: 99%

“…Although a few studies have shown the value of transmission electron microscopy (TEM) to investigate not only human, but also animal ultrastructure, not many researchers have used scanning electron microscopy (SEM) to study platelet and fibrin ultrastructure. Studies done include animals ranging from rhinoceros, elephant, and bovine species (du Plessis and Stevens, 2002;du Plessis et al 1996;Liu et al, 1998;Zucker-Franklin et al, 1985). Liu et al (1998) studied the effect of procainamide on the ultrastructure of human blood platelets.…”

Section: Introductionmentioning

confidence: 99%

“…du Plessis and Stevens (2002) studied internal platelet ultrastructure of the African elephant, using the light microscope, SEM, and TEM. Blood was collected from animals and blood smears were made, and showed that platelets have numerous pseudopodia.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparative Ultrastructural Analyses of Mouse, Rabbit, and Human Platelets and Fibrin Networks

Pretorius

Humphries

Ekpo

et al. 2007

Microscopy Res & Technique

View full text Add to dashboard Cite

Platelets and fibrin play an important role in the coagulation process, where they are involved in the maintenance of hemostasis. Fibrin dysfunction is associated with the development of vascular complications, while proneness to the formation of tight and rigid fibrin networks is independently associated with thrombotic disease. Here we investigate the ultrastructure of human, rabbit, and mouse platelets and fibrin networks, using the scanning electron microscope. Human and rabbit fibrin and platelets, with regards to morphology as well as size of major and minor fibers compare well with each other. However, mouse fibers are much thinner and form a flimsy branching network. Platelet aggregate morphology of all three species compare well with each other. We conclude that rabbit platelet and fibrin networks could be used successfully when studying the effect of pharmaceutical products in preclinical trials, when looking at the effects of these products on morphology and ultrastructure.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Ultrastructural Analyses of Mouse, Rabbit, and Human Platelets and Fibrin Networks

Pretorius

Humphries

Ekpo

et al. 2007

Microscopy Res & Technique

View full text Add to dashboard Cite

show abstract

“…The automated analyzer did not accurately quantitate elephant platelets, presumably due to their small size, and resulted in consistently lower platelet counts compared to the estimates by blood film review. Accurate platelet estimates are important clinically and may be challenging to obtain in elephants as they are small and readily activated which can result in clumping (Plessis & Stevens 2002;Stacy & Hollinger 2018). Inflammation and neoplasia are the most common causes of thrombocytosis in dogs (Neel et al 2012).…”

Section: Discussionmentioning

confidence: 99%

The importance of manual white blood cell differential counts and platelet estimates in elephant hematology: blood film review is essential

Weisbrod

Isaza

Cray

et al. 2021

Veterinary Quarterly

View full text Add to dashboard Cite

“…Bovine, rhinoceros, and elephant platelets have also been studied using the TEM, but these platelets were not activated (du Plessis and Stevens, 2002;du Plessis et al, 1996;Zucker-Franklin et al, 1985). Inactivated platelet morphology of buffalo (Syncerus caffer), bovine (Bos taurus), and human platelets have also been compared (du Plessis et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of activated mouse platelets: A qualitative scanning electron microscopy study

Pretorius

Oberholzer

Smit

2008

Microscopy Res & Technique

View full text Add to dashboard Cite

Platelets form an integral part of the coagulation process, and their ultrastructure can provide valuable information regarding diseases associated with hemostasis. During coagulation, platelets aggregate; this aggregation can be achieved in vitro, by adding thrombin to platelet-rich plasma. Previous research showed that human thrombin could be used successfully to activate mouse platelets. When conservative changes are included, the amino acid similarity between human and mouse thrombin is approximately 75%. In this qualitative study, we compare the ultrastructure of mouse platelet aggregates activated by human thrombin as well as two concentrations of mouse thrombin, using the scanning electron microscope. Results show that both human and mouse thrombin activate platelets to form aggregates with typical pseudopodia formation. Magnification up to 250,000x showed membrane morphology with the open canalicular system pores visible in both the mouse- and human-activated platelets. It is therefore concluded that mouse platelets can be successfully aggregated using either mouse or human thrombin.

show abstract

Blood Platelets of the African Elephant

Cited by 12 publications

References 6 publications

Comparative Ultrastructural Analyses of Mouse, Rabbit, and Human Platelets and Fibrin Networks

Comparative Ultrastructural Analyses of Mouse, Rabbit, and Human Platelets and Fibrin Networks

The importance of manual white blood cell differential counts and platelet estimates in elephant hematology: blood film review is essential

Ultrastructure of activated mouse platelets: A qualitative scanning electron microscopy study

Contact Info

Product

Resources

About