Blood sampling affects circulating TIMP‐1 concentration, a useful biomarker in estimating liver fibrosis stages†

Mannello, Ferdinando; Jung, Klaus

doi:10.1002/hep.22360

Cited by 6 publications

(2 citation statements)

References 10 publications

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“…In addition, previous investigation confirmed that TGF-b1 is a critical regulator of fibrogenesis in chronic liver diseases (Bissell et al, 2001). Besides, the levels of TIMP-1 seemed to be firmly related with the pathological degree of liver fibrosis in patients, and suppress the expression of TIMP-1 can attenuate the progression of liver fibrosis (Nie et al, 2001;Mannello and Jung, 2008). In our study, it was found that treatment of rats with ICQA could significantly and dose -dependently inhibit the expression of COL1a1, TGF-b1 and TIMP-1 at both mRNA and protein levels, which indicated the potential therapeutic action of ICQA for liver fibrosis.…”

Section: Discussionmentioning

confidence: 81%

Isochlorogenic Acid A Attenuates the Progression of Liver Fibrosis Through Regulating HMGB1/TLR4/NF-κB Signaling Pathway

et al. 2020

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Liver fibrosis, a chronic damage process related to further progression of hepatic cirrhosis, has yet no truly effective treatment. Isochlorogenic acid A (ICQA), isolated from a traditional Chinese herbal medicine named Laggera alata (DC.) Sch.Bip. ex Oliv. (Asteraceae), is proved to exhibit anti-inflammatory, hepatoprotective and antiviral properties. However, the actions of ICQA on liver fibrosis are poorly understood. The purpose of this study was to evaluate the actions of ICQA on liver fibrosis and clarify the underlying mechanism. It was found that ICQA had significant protective actions on liver injury, inflammation as we as fibrosis in rats. Meanwhile, ICQA prevented hepatic stellate cells (HSC) activation, indicated by its inhibitory effect on the overexpression of a-smooth muscle actin (a-SMA). In addition, the reduced fibrosis was found to be associated with the decreased protein expression of high-mobility group box 1 (HMGB1) as well as toll like receptor (TLR) 4. Simultaneously, ICQA can suppress the cytoplasmic translocation of HMGB1 in rat liver. Further investigations indicated that ICQA treatment dramatically attenuated the nuclear translocation of the nuclear factor-kB (NF-kB) p65 and suppressed the hepatic expression of p−IkBa in rats with liver fibrosis. Taken together, our study indicated that ICQA could protect against CCl 4-induced liver fibrosis probably through suppressing the HMGB1/TLR4/NF-kB signaling pathways.

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Section: Discussionmentioning

confidence: 81%

Isochlorogenic Acid A Attenuates the Progression of Liver Fibrosis Through Regulating HMGB1/TLR4/NF-κB Signaling Pathway

et al. 2020

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“…However, a note of caution is that blood collection procedures may affect the ability to detect some of these proteins in blood. This limitation is known for TIMP1 and other metalloproteinases (Jung et al, 2008; Losanoff et al, 2008; Mannello, 2008; Mannello & Jung, 2008; Mannello et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Protein signatures of centenarians and their offspring suggest centenarians age slower than other humans

et al. 2021

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Using samples from the New England Centenarian Study (NECS), we sought to characterize the serum proteome of 77 centenarians, 82 centenarians' offspring, and 65 age‐matched controls of the offspring (mean ages: 105, 80, and 79 years). We identified 1312 proteins that significantly differ between centenarians and their offspring and controls (FDR < 1%), and two different protein signatures that predict longer survival in centenarians and in younger people. By comparing the centenarian signature with 2 independent proteomic studies of aging, we replicated the association of 484 proteins of aging and we identified two serum protein signatures that are specific of extreme old age. The data suggest that centenarians acquire similar aging signatures as seen in younger cohorts that have short survival periods, suggesting that they do not escape normal aging markers, but rather acquire them much later than usual. For example, centenarian signatures are significantly enriched for senescence‐associated secretory phenotypes, consistent with those seen with younger aged individuals, and from this finding, we provide a new list of serum proteins that can be used to measure cellular senescence. Protein co‐expression network analysis suggests that a small number of biological drivers may regulate aging and extreme longevity, and that changes in gene regulation may be important to reach extreme old age. This centenarian study thus provides additional signatures that can be used to measure aging and provides specific circulating biomarkers of healthy aging and longevity, suggesting potential mechanisms that could help prolong health and support longevity.

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Cytokine functions of TIMP-1

Ries

2013

Cell. Mol. Life Sci.

239

213

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The tissue inhibitors of metalloproteinases (TIMPs) are well recognized for their role in extracellular matrix remodeling by controlling the activity of matrix metalloproteinases (MMPs). Independent of MMP inhibition, TIMPs act as signaling molecules with cytokine-like activities thereby influencing various biological processes including cell growth, apoptosis, differentiation, angiogenesis, and oncogenesis. Recent studies on TIMP-1's cytokine functions have identified complex regulatory networks involving a specific surface receptor and subsequent signaling pathways including miRNA-mediated posttranscriptional regulation of gene expression that ultimately control the fate and behavior of the cells. The present review summarizes the current knowledge on TIMP-1 as a cytokine modulator of cell functions, outlines recent progress in defining molecular pathways that transmit TIMP-1 signals from the cell periphery into the nucleus, and discusses TIMP-1's role as a cytokine in the pathophysiology of cancer and other human diseases.

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Blood sampling affects circulating TIMP‐1 concentration, a useful biomarker in estimating liver fibrosis stages†

Cited by 6 publications

References 10 publications

Isochlorogenic Acid A Attenuates the Progression of Liver Fibrosis Through Regulating HMGB1/TLR4/NF-κB Signaling Pathway

Isochlorogenic Acid A Attenuates the Progression of Liver Fibrosis Through Regulating HMGB1/TLR4/NF-κB Signaling Pathway

Protein signatures of centenarians and their offspring suggest centenarians age slower than other humans

Cytokine functions of TIMP-1

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