Blood sampling techniques in reptiles

Samour, H.J.; Risley, D; March, T; Savage, B.; Nieva, O; Jones, DM

doi:10.1136/vr.114.19.472

Cited by 32 publications

(9 citation statements)

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“…Erythrocytes are morphologically similar among various species of reptiles [30]. The mature erythrocytes were nucleated ellipsoidal cells with pink stained cytoplasm, which are consistent with that reported in desert tortoise [13], in European pond turtles [24] and in green turtles [31]. The erythrocyte measurements are also in agreement with the results of Colagar and Jafari [32] and Ugˇurtas et al [23].…”

Section: Discussionsupporting

confidence: 82%

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Kassab¹,

Shousha²,

Fargani³

2009

Open Anat J

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Abstract:The morphological characteristics of peripheral blood cells, the anatomical structure of the liver and spleen were examined in desert tortoise. Forty healthy adult Testudo graeca tortoises, 20 females and 20 males were selected for this study. The tortoises were given thorough examination, and a blood sample was collected from the heart by cardiocentesis. The Wright staining method was used for classification of the blood cells. Seven different types of blood cells were determined: erythrocytes, heterophils, eosinophils, basophils, lymphocytes, monocytes and thrombocytes. Mature erythrocytes of captive Testudo graeca were nucleated ellipsoidal cells. Erythrocytes measurements for males were (length 18.76 ± 2.81 m width 9.52 ± 2.23 m) and for females were (length 19.19 ± 2.90 m width 9.84 ± 2.50 m). The heterophil contain large, eosinophilic, ovoid, cytoplasmic granules with eccentric nucleus. The eosinophil is distinguished by its round, eosinophilic cytoplasmic granules and the nucleus is round to oval, single or bi-lobed, and eccentrically placed within the cytoplasm. The basophil was easily identified by its deeply stained purple, large, round granules that remained tightly adhered to the centrally located nucleus. The lymphocyte contained a small amount of blue staining cytoplasm and a round nucleus with a fine reticular pattern. The monocyte contains a large amount of light bluegray, finely granular or vacuolated cytoplasm, and an oval or indented nucleus. Thrombocytes were oval-shaped cells contained round, densely stained nucleus. The liver composed of two lobes, right and left which were connected with a narrow band of connective tissue. The parenchyma of the tortoise liver is seen to be composed of intersinusoidal cords of hepatocytes. The spleen grossly appeared as round or oval structure. The parenchyma (splenic pulp) formed of lymphoid tissue and is of two distinct types; white and red pulps.Overall, this study provides identification of the morphological characteristics of different peripheral blood cells of the tortoise Testudo graeca species as well as the anatomy of the liver and spleen, as a reference for future hematological studies of this species and may be used as a basis for comparison in clinical cases.

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Section: Discussionsupporting

confidence: 82%

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Kassab¹,

Shousha²,

Fargani³

2009

Open Anat J

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“…Estudos sobre os parâmetros hematológicos e bioquímicos do sangue têm sido realizados em outras espécies de quelônios em várias regiões do mundo (ROSSKOPF JÚNIOR e WOERPEL, 1982;TAYLOR JÚNIOR e JACOBSON, 1992;SAMOUR et al, 1984;BORDEAU, 1989;MARKS e CINTINO, 1990;PAGÉS et al, 1992;RAPHAEL et al, 1994). No Brasil, segundo a literatura indexada disponível, estudos sobre os parâmetros bioquímicos do sangue com Podocnemis expansa foram realizados por MUNDIM et al (1999).…”

Section: Introductionunclassified

VARIAÇÃO DOS CONSTITUINTES BIOQUÍMICOS SANGUÍNEOS DE TARTARUGAS-DA-AMAZÔNIA (Podocnemis expansa, Schweigger 1812) (TESTUDINATA) MANTIDAS EM CRIATÓRIO COMERCIAL

Santos¹,

Malta²,

Mundim³

et al. 2005

AVS

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O objetivo desta pesquisa foi avaliar as variações dos constituintes bioquímicos sanguíneos da tartaruga Podocnemis expansa de um criatório comercial. Para isto, foram colhidas, por venipuntura do seio vertebral caudal, amostras de sangue de 46 animais mantidos em tanques na Fazenda Moenda do Lago, distrito de São José dos Bandeirantes, município de Nova Crixás GO. Após colhidas, as amostras de sangue foram transferidas rapidamente para frascos vacutainer® sem anticoagulante e levados à centrífuga para obtenção do soro, logo após transferidos para eppendorf e armazenados a 200C. O material foi transportado para o laboratório da Faculdade de Medicina Veterinária (FAMEV) da Universidade Federal de Uberlândia (UFU) para o seu processamento. As análises foram processadas colorimetricamente e em sensor rápido de glicose (Precision Plus Medsense®), os valores médios foram: proteínas totais 4,42±0,55 g/ dL, albumina 2,50±0,2 g/dL, globulinas 1,92±0,35 g/dL, relação albumina/globulinas (A/G) 1,55±1,46, glicose 122,9±35,2 mg/dL, uréia 61,13±28,35 mg/dL, creatinina 0,098±0,021 mg/dL, ácido úrico 2,08±0,53 mg/dL, cálcio total 7,08±1,04 mg/dL, cálcio ionizado 4,41±0,60 mg/ dL, colesterol 106,93±21,03 mg/dL, fósforo 5,21±1,22 mg/dL, relação cálcio/fósforo (Ca++/P) 1,58±0,66, ferro 390,35±116,65 ì g/dL, magnésio 1,59±0,14 mg/dL, cloretos 86,40±7,63 mEq/L, aspartato aminotransferase (AST) 194,56±154,27 U/L, alanino aminotransferase (ALT) 4,04±3,00 U/L, fosfatase alcalina 131,13±59,96 U/L, gama glutamiltransferase (GGT) 0,60±0,84 U/L e triglicérides 127,65±100,86 mg/dL. Conclui-se que os valores apresentam grandes diferenças dos valores de outras espécies de quelônios e que a relação albumina:globulina, as concentrações plasmáticas de ácido úrico e glicose são inversamente proporcionais às massas corporais dos animais. A espécie estudada apresenta escassos valores de bioquímica sanguínea na literatura, desta maneira os dados que foram apresentados auxiliam no estabelecimento de um padrão de bioquímica sanguínea para Podocnemis expansa criadas em cativeiro, mas também pode ser base para novos estudos.

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“…Accurate interpretation of clinical laboratory values depends on the reliability of the technical aspect of the procedure and on blood collection techniques and preanalytic conditions of blood storage. A variety of blood collection sites and techniques have been described for chelonians 1–4 . Jacobson et al 4 reported on blood sampling and sample handling techniques in desert tortoises.…”

mentioning

confidence: 99%

Effects of Blood Processing Techniques on Sodium and Potassium Values: A Comparison Between Aldabra Tortoises (Geochelone gigantea) and Burmese Mountain Tortoises (Manouria emys)

Abou‐Madi

Jacobson

2003

Veterinary Clinical Pathol

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Blood sampling techniques in reptiles

Cited by 32 publications

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Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

VARIAÇÃO DOS CONSTITUINTES BIOQUÍMICOS SANGUÍNEOS DE TARTARUGAS-DA-AMAZÔNIA (Podocnemis expansa, Schweigger 1812) (TESTUDINATA) MANTIDAS EM CRIATÓRIO COMERCIAL

Effects of Blood Processing Techniques on Sodium and Potassium Values: A Comparison Between Aldabra Tortoises (Geochelone gigantea) and Burmese Mountain Tortoises (Manouria emys)

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Blood sampling techniques in reptiles

Cited by 32 publications

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Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

Morphology of Blood Cells, Liver and Spleen of the Desert Tortoise (Testudo graeca)

VARIAÇÃO DOS CONSTITUINTES BIOQUÍMICOS SANGUÍNEOS DE TARTARUGAS-DA-AMAZÔNIA (Podocnemis expansa, Schweigger  1812) (TESTUDINATA) MANTIDAS EM CRIATÓRIO COMERCIAL

Effects of Blood Processing Techniques on Sodium and Potassium Values: A Comparison Between Aldabra Tortoises (Geochelone gigantea) and Burmese Mountain Tortoises (Manouria emys)

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VARIAÇÃO DOS CONSTITUINTES BIOQUÍMICOS SANGUÍNEOS DE TARTARUGAS-DA-AMAZÔNIA (Podocnemis expansa, Schweigger 1812) (TESTUDINATA) MANTIDAS EM CRIATÓRIO COMERCIAL