Blueberry Leaf Extract Prevents Lacrimal Hyposecretion in Sjögren’s Syndrome-like Model of Non-obese Diabetic Mice

Ogawa, Kishiko; Urata, Karin; Maeda, Saki; Ohno, Yuta; Satoh, Kazue; Yamada, Yoshiyuki; Suzuki, Yosuke; Koga, Yasuko; Sugamoto, Kazuhiro; Kawaguchi, Makiko; Kunitake, Hisato; Nishiyama, Kazuo; Goto, Yo; Nakayama, Takayuki; Yamasaki, Masao

doi:10.21873/invivo.13064

Cited by 4 publications

(13 citation statements)

References 41 publications

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“…18,19 Tears are necessary to prevent dryness, shield the eye's surface from irritants, and feed the cells with oxygen and nutrients. 20 They are also necessary for the proper refraction of light to achieve clear vision. An abnormality of the ocular surface, such as dryness and lipid membrane disintegration, characterizes SS, a chronic, multifactorial eye disorder.…”

Section: Normal Anatomy and Function Of Lacrimal Glandmentioning

confidence: 99%

“…Tears include a variety of immunologically active proteins, peptides, and glycoproteins, which fight germs and keep tears from drying out 18,19 . Tears are necessary to prevent dryness, shield the eye's surface from irritants, and feed the cells with oxygen and nutrients 20 . They are also necessary for the proper refraction of light to achieve clear vision.…”

Section: Introductionmentioning

confidence: 99%

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Advances in imaging of the lacrimal gland in Sjögren's syndrome: A narrative review

Zhong,

Zhang,

Ran

2023

J of Clinical Ultrasound

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Due to lymphocytic infiltration of the salivary and lacrimal glands, Sjogren's syndrome (SS), a systemic autoimmune illness that mostly affects the exocrine glands, causes dry mouth (xerostomia) and dry eyes (xerophthalmia). Additionally, SS is associated with various comorbidities such as cardiovascular diseases, infections, musculoskeletal diseases, and cancers. Among patients with SS, xerophthalmia frequently arises as a complication, leading to insufficient tear production or rapid tear evaporation, thereby causing discomfort, irritation, and a gritty sensation in the eyes. This article aims to examine recent advancements in the imaging of the lacrimal gland in Sjögren's syndrome and briefly discusses the utilization of various imaging examinations for the lacrimal gland in this particular disease.

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Section: Normal Anatomy and Function Of Lacrimal Glandmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advances in imaging of the lacrimal gland in Sjögren's syndrome: A narrative review

Zhong,

Zhang,

Ran

2023

J of Clinical Ultrasound

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“…In view of the above, work on finding effective, easily cultivated substitutes with proven effects, i.e., chokeberry, honeysuckle berry, seems to be highly advisable. Work on finding substitutes for bilberry fruit was carried out by Ogawa et al, where the authors investigated the possibility of using the more accessible leaves of this plant [22].…”

Section: Introductionmentioning

confidence: 99%

Extract from Aronia melanocarpa, Lonicera caerulea, and Vaccinium myrtillus Improves near Visual Acuity in People with Presbyopia

Szumny,

Kucharska,

Czajor

et al. 2024

Nutrients

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Presbyopia is a global problem with an estimated 1.3 billion patients worldwide. In the area of functional food applications, dietary supplements or herbs, there are very few reports describing the positive effects of their use. In the available literature, there is a lack of studies in humans as well as on an animal model of extracts containing, simultaneously, compounds from the polyphenol group (in particular, anthocyanins) and iridoids, so we undertook a study of the effects of a preparation composed of these compounds on a condition of the organ of vision. Our previous experience on a rabbit model proved the positive effect of taking an oral extract of Cornus mas in stabilizing the intraocular pressure of the eye. The purpose of this study was to evaluate the effect of an orally administered ternary compound preparation on the status of physiological parameters of the ocular organ. The preparation contained an extract of the chokeberry Aronia melanocarpa, the honeysuckle berry Lonicera caerulea L., and the bilberry Vaccinium myrtillus (hereafter AKB) standardized for anthocyanins and iridoids, as bioactive compounds known from the literature. A randomized, double-blind, cross-over study lasting with a “wash-out” period of 17 weeks evaluated a group of 23 people over the age of 50, who were subjects with presbyopia and burdened by prolonged work in front of screen monitors. The group of volunteers was recruited from people who perform white-collar jobs on a daily basis. The effects of the test substances contained in the preparation on visual acuity for distance and near, sense of contrast for distance and near, intraocular pressure, and conjunctival lubrication, tested by Schirmer test, LIPCOF index and TBUT test, and visual field test were evaluated. Anthocyanins (including cyanidin 3-O-galactoside, delphinidin 3-O-arabinoside, cyanidin 3-O-glucoside, cyanidin 3-O-rutinoside, cyanidin 3-O-arabinoside) and iridoids (including loganin, sweroside, loganic acid) were identified as substances present in the extract obtained by HPLC-MS. The preliminary results showed that the composition of AKB applied orally does not change visual acuity in the first 6 weeks of administration. Only in the next cycle of the study was an improvement in near visual acuity observed in 92.3% of the patients. This may indicate potential to correct near vision in presbyopic patients. On the other hand, an improvement in conjunctival wetting was observed in the Schirmer test at the beginning of week 6 of administration in 80% of patients. This effect was weakened in subsequent weeks of conducting the experiment to 61.5%. The improvement in conjunctival hydration in the Schirmer test shows the potential beneficial effect of the AKB formulation in a group of patients with dry eye syndrome. This is the first study of a preparation based on natural, standardized extracts of chokeberry, honeysuckle berry, and bilberry. Preliminary studies show an improvement in near visual acuity and conjunctival hydration on the Schirmer test, but this needs to be confirmed in further studies.

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“…We reported the first evidence of the cytoprotective effect of BStEx against blue light-induced retinal cell damage in the field of eye health (16). In addition, we recently revealed that BLEx has a preventive effect on lacrimal hyposecretion (15).…”

mentioning

confidence: 96%

“…Additionally, it has been reported that BStEx contains proanthocyanidins with a relatively lower degree of polymerization than BLEx (14). Furthermore, BStEx and BLEx contain other phenolic compounds such as catechins, epicatechins, chlorogenic acid, quinic acid, and caffeic acid (15,16). Previous studies have shown that BStEx inhibits lipid synthesis in the liver (17), increases anti-adult T-cell leukemia (ATL) activity (18), and promotes viral proliferation (14).…”

mentioning

confidence: 99%

Blueberry Stem Extract Prevents Lacrimal Hyposecretion in Non-obese Diabetic MiceviaActivation of AMPK

Ogawa

Ohno

Tagashira

et al. 2023

In Vivo

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Background/Aim: Tears secreted from the lacrimal gland are essential for preserving the ocular surface. Thus, dysfunction of the lacrimal gland in Sjögren's syndrome (SS) can lead to dry eye, resulting in a reduced quality of life. We previously reported that blueberry 'leaf ' water extract prevents lacrimal hyposecretion in male non-obese diabetic (NOD) mice in a SS-like model. In this study, we investigated the effect of blueberry 'stem' water extract (BStEx) on lacrimal hyposecretion in NOD mice. Materials and Methods: Male NOD mice were fed 1% BStEx or control (AIN-93G) for 2, 4, or 6 weeks from 4 weeks of age. Pilocarpine-induced tear secretion was measured using a phenol red-impregnated thread. The lacrimal glands were histologically evaluated by HE staining. Inflammatory cytokine levels in the lacrimal glands were measured using ELISA. Immunostaining was performed to examine aquaporin 5 (AQP5) localization. The expression levels of autophagy-related proteins, AQP5, and phosphorylated AMPK were measured using western blotting. Results: After feeding BStEx to mice for 4 or 6 weeks, tear volume was observed to have increased in the BStEx group compared with that in the control group. There were no significant differences in inflammatory cell infiltration, autophagy-related protein expression, or the localization and expression of AQP5 in the lacrimal glands between the two groups. In contrast, AMPK phosphorylation increased in the BStEx group. Conclusion: BStEx prevented lacrimal hyposecretion in the SS-like model of male NOD mice, probably by opening tight junctions via the activation of AMPK in lacrimal acinar cells.Tears secreted from the lacrimal gland are essential for preserving the ocular surface because of their physiological functions of wetting the cornea and sclera, providing oxygen and nutrition, and protecting against bacteria and foreign substances. Tears are also necessary for clear vision because light must be correctly refracted through tears. Thus, dysfunction of the lacrimal gland can lead to a reduced number of tears and, in turn, dry eye, resulting in a reduced quality of life. There are several causes of lacrimal dysfunction, including Sjögren's syndrome (SS) (1), radiotherapy for head and neck tumors (2), and metabolic syndrome (3). In addition, the increased amount of time spent working with visual display terminals, such as computers and smartphones, has the potential to cause lacrimal dysfunction and dry eye (4, 5).Primary SS is an autoimmune disease accompanied by the infiltration of inflammatory cells into exocrine glands, such as 1003

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Blueberry Leaf Extract Prevents Lacrimal Hyposecretion in Sjögren’s Syndrome-like Model of Non-obese Diabetic Mice

Cited by 4 publications

References 41 publications

Advances in imaging of the lacrimal gland in Sjögren's syndrome: A narrative review

Advances in imaging of the lacrimal gland in Sjögren's syndrome: A narrative review

Extract from Aronia melanocarpa, Lonicera caerulea, and Vaccinium myrtillus Improves near Visual Acuity in People with Presbyopia

Blueberry Stem Extract Prevents Lacrimal Hyposecretion in Non-obese Diabetic MiceviaActivation of AMPK

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