BMP-7, MMP-9, and TGF-β tissue remodeling proteins and their correlations with interleukins 6 and 10 in chronic rhinosinusitis

Lucas, Bernardo Relvas; Voegels, Richard Louis; Amaral, Jônatas Bussador do; Bachi, André Luís Lacerda; Pezato, Rogério

doi:10.1007/s00405-021-06722-8

Cited by 12 publications

(11 citation statements)

References 43 publications

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“…56 Recent discoveries about an altered presence of ECM components, tissue remodeling proteins, and adhesion molecules in different forms of CRS underline our result. 9,10,57,58 P-selectin is known to be expressed mainly by activated platelets and endothelial cells. It belongs to the family of selectins, a group of adhesion molecules that play an essential role in immunity, inflammatory responses, and tissue healing.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue remodeling along with fibrosis is a concomitant issue in CRSsNP. 9,55 In this process components of the ECM are decomposed by shedding to enable cell movement and reconstruction. 56 Recent discoveries about an altered presence of ECM components, tissue remodeling proteins, and adhesion molecules in different forms of CRS underline our result.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, these previous studies have often been restricted by the limited range of proteins tested. 4,[8][9][10][11][12][13][14] Conversely, large-scale analysis of the CRSsNP proteome allows previously unknown disease factors to be revealed, more insight into the pathophysiology to be gained, and novel potential therapeutic targets to be identified. The use of nasal mucus provides an easy, non-invasive, and painless way of biosignature sampling.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, these previous studies have often been restricted by the limited range of proteins tested. 4,8–14…”

Section: Introductionmentioning

confidence: 99%

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Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus

Pesold

Wendler

Morgenthaler

et al. 2022

Am J Rhinol�Allergy

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Background Chronic rhinosinusitis without nasal polyps (CRSsNP) represents a phenotype of CRS, whose immunological mechanisms are still unclear. So far there are neither suitable biomarkers to determine the course of the disease nor an individual therapy. Objective The purpose of this study was to characterize the CRSsNP endotype by identifying and validating non-invasive proteomic biomarkers. Methods A highly-multiplexed proteomic array consisting of antibodies against 2000 proteins was used to identify proteins that are differentially expressed in the nasal mucus of the CRSsNP and control groups (n = 7 per group). The proteins identified to be most differentially expressed were validated in matched nasal mucus samples using western blots and enzyme-linked immunosorbent assay (ELISA). Validation was also done in a second cohort using western blots (CRSsNP n = 25, control n = 23) and ELISA (n = 30 per group). Additionally, immunohistochemistry in CRSsNP and control tissue samples was performed to characterize the selected proteins further. Results Out of the 2000 proteins examined, 7 from the most differentially expressed proteins were chosen to be validated. The validation results showed that 4 proteins were significantly upregulated in CRSsNP mucus, including macrophage inflammatory protein-1beta (MIP-1β), resistin, high mobility group box 1 (HMGB1), and forkhead box protein 3 (FOXP3). Cartilage acidic protein 1 (CRTAC1) was not significantly upregulated. Two proteins were significantly downregulated including scavenger receptor class F member 2 (SCARF2) and P-selectin. All proteins selected are mainly associated with inflammation, cell proliferation/differentiation, apoptosis and cell–cell or cell–matrix interaction. Conclusion Proteomic analysis of CRSsNP and control mucus has confirmed known and revealed novel disease-associated proteins that could potentially serve as a new biosignature for CRSsNP. Analysis of the associated pathways will specify endotypes of CRSsNP and will lead to an improved understanding of the pathophysiology of CRSsNP. Furthermore, our data contribute to the development of a reproducible, non-invasive, and quantitative “liquid biopsy” for rhinosinusitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Moreover, these previous studies have often been restricted by the limited range of proteins tested. 4,8–14…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus

Pesold

Wendler

Morgenthaler

et al. 2022

Am J Rhinol�Allergy

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“…However, in a study conducted in Korea, the serum IL-10 level in CRSwNP patients was significantly lower than that in healthy people, but no difference was observed between eosinophilic CRSwNP and those with non-eosinophilic CRSwNP ( 34 ). In new research from Brazil, the level of IL-10 protein in nasal polyps was found to be significantly lower than that in mucosa from the control group ( 35 ). IL-10 level was inversely correlated with olfactory function in patients with CRS ( 36 ).…”

Section: Differential Expression Of Il-10 Family Cytokines In Individ...mentioning

confidence: 93%

IL-10 family cytokines in chronic rhinosinusitis with nasal polyps: From experiments to the clinic

Xuan

Zhang²,

Wang³

et al. 2022

Front. Immunol.

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Chronic rhinosinusitis with nasal polyps (CRSwNP) is considered a nasal sinus inflammatory disease that can be dominated by immune cells and cytokines. IL-10 family cytokines exert essential functions in immune responses during infection and inflammation. Recently, the understanding of the roles of the IL-10 family in CRSwNP is being reconsidered. IL-10 family members are now considered complex cytokines that are capable of affecting epithelial function and involved in allergies and infections. Furthermore, the IL-10 family responds to glucocorticoid treatment, and there have been clinical trials of therapies manipulating these cytokines to remedy airway inflammatory diseases. Here, we summarize the recent progress in the understanding of IL-10 family cytokines in CRSwNP and suggest more specific strategies to exploit these cytokines for the effective treatment of CRSwNP.

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Comparison of mucus and serum biomarker sampling in chronic rhinosinusitis with nasal polyps

Mueller,

Wendler,

Mayr

et al. 2023

Int Forum Allergy Rhinol

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ObjectiveThe objective of this study was to analyze advantages and disadvantages of mucus and serum for biomarker analysis.MethodsThis study includes prospective study of 61 CRS with nasal polyps patients who were followed over 24 months and over nine time points after functional endoscopic sinus surgery. At each time points, the nasal polyp score (NPS) was assessed and mucus as well as serum was collected. Selected were measured in mucus and serum. Mean, standard deviation and variance, undetectable values, and the correlation of the biomarkers to the NPS over time and to early recurrences were calculated, and the effect of surgery on the biomarkers was assessed. Additionally, the diurnal rhythm of all biomarkers was measures in order to assure stable biomarker values during sampling times.ResultsAll biomarkers showed stable values during sampling times. Serum biomarker levels displayed higher percentages of undetectable values compared to mucus biomarkers. Mucus periostin (p < 0.001, r = 0.89), mucus IgE (p < 0.001, r = 0.51), serum periostin (p < 0.001, r = 0.53), mucus CST1 (p < 0.001, r = 0.27), and serum IgE (p < 0.01, r = −0.18) were the best marker and medium combinations to track the NPS over time and to predict recurrences. Mucus serpinF2 was negatively correlated and predicted early recurrences (p = 0.026, R2 = 0.015).ConclusionsSerum and mucus both represent viable mediums for “liquid biopsies.” The most promising biomarker/medium combinations over time to track disease severity were mucus periostin, mucus IgE, serum periostin, mucus CST1, and serum IgE. Mucus serpinF2 was the best biomarker to predict early recurrences.

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BMP-7, MMP-9, and TGF-β tissue remodeling proteins and their correlations with interleukins 6 and 10 in chronic rhinosinusitis

Cited by 12 publications

References 43 publications

Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus

Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus

IL-10 family cytokines in chronic rhinosinusitis with nasal polyps: From experiments to the clinic

Comparison of mucus and serum biomarker sampling in chronic rhinosinusitis with nasal polyps

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