BmSUC1 is essential for glycometabolism modulation in the silkworm, Bombyx mori

Gan, Quan; Zhang, Xinwei; Zhang, Daobo; Shi, Liang; Zhou, Yue; Sun, Tao; Jiang, Song; Gao, Junshan; Meng, Yan

doi:10.1016/j.bbagrm.2018.04.002

Cited by 16 publications

(47 citation statements)

References 53 publications

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“…Only monosaccharides of the end products of carbohydrate digestion are absorbed and utilized. The sugar absorbed and metabolized is essential for silkworm larval development and cocoon production [51]. The initial digestion of carbohydrates is mediated by amylases and only alpha-amylases have the activity to catalyze starch and glycogen in insects [10,52].…”

Section: Discussionmentioning

confidence: 99%

“…Smaller polysaccharides and disaccharides produced by catalysis of α-amylase are further degraded to monosaccharides during subsequent steps of carbohydrate digestion performed by distinct enzymes [10,53]. After the initial breakdown of large oligosaccharides by amylases, α-glucosidase and β-fructofuranosidase mediate the subsequent degradation of oligo-and disaccharides into monosaccharides [10,51]. Sugar mimicking alkaloids, such as 1,4-dideoxy-1,4-imino-D-arabinitol (D-AB1) and 1-deoxynojirimycin (DNJ) derived from mulberry latex, are strong inhibitors of α-glucosidase but do not exhibit inhibitory activity against β-fructofuranosidase (β-FFase) [54,55].…”

Section: Discussionmentioning

confidence: 99%

“…Sugar mimicking alkaloids, such as 1,4-dideoxy-1,4-imino-D-arabinitol (D-AB1) and 1-deoxynojirimycin (DNJ) derived from mulberry latex, are strong inhibitors of α-glucosidase but do not exhibit inhibitory activity against β-fructofuranosidase (β-FFase) [54,55]. BGIBMGA005696 (BmSuc1) was upregulated in DJ48/0h and DJ48/24h that is a novel animal β-FFase cloned and identified in B. mori [51,56]. BmSuc1 acts as an essential sucrase by directly modulating the degree of sucrose hydrolysis and silencing BmSuc1, significantly reducing glucose in the midgut and leading to smaller body size, lighter weight, and developmental delays of silkworm larvae [51].…”

Section: Discussionmentioning

confidence: 99%

“…BGIBMGA005696 (BmSuc1) was upregulated in DJ48/0h and DJ48/24h that is a novel animal β-FFase cloned and identified in B. mori [51,56]. BmSuc1 acts as an essential sucrase by directly modulating the degree of sucrose hydrolysis and silencing BmSuc1, significantly reducing glucose in the midgut and leading to smaller body size, lighter weight, and developmental delays of silkworm larvae [51].…”

Section: Discussionmentioning

confidence: 99%

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iTRAQ-Based Quantitative Proteomic Analysis of Digestive Juice across the First 48 Hours of the Fifth Instar in Silkworm Larvae

Zhang

Qian

et al. 2019

IJMS

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The silkworm is an oligophagous insect for which mulberry leaves are the sole diet. The nutrients needed for vital activities of the egg, pupal, and adult stages, and the proteins formed in the cocoon, are all derived from the larval stages. The silkworm feeds and grows quickly during the larval stages. In particular, the amount of leaf ingested and digested quickly increases from the ecdysis to the gluttonous stage in the fifth instar period. In this study, we used the iTRAQ proteomic technique to identify and analyze silkworm larval digestive juice proteins during this period. A total of 227 proteins were successfully identified. These were primarily serine protease activity, esterase activity, binding, and serine protease inhibitors, which were mainly involved in the digestion and overcoming the detrimental effects of mulberry leaves. Moreover, 30 genes of the identified proteins were expressed specifically in the midgut. Temporal proteomic analysis of digestive juice revealed developmental dynamic features related to molecular mechanisms of the principal functions of digesting, resisting pathogens, and overruling the inhibitory effects of mulberry leaves protease inhibitors (PIs) with a dynamic strategy, although overruling the inhibitory effects has not yet been confirmed by previous study. These findings will help address the potential functions of digestive juice in silkworm larvae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

iTRAQ-Based Quantitative Proteomic Analysis of Digestive Juice across the First 48 Hours of the Fifth Instar in Silkworm Larvae

Zhang

Qian

et al. 2019

IJMS

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“…The nucleoprotein and cytoprotein of Sf9 cells in the subcellular localization experiment were extracted using the Membrane, Nuclear and Cytoplasmic Protein Extraction kit (Sangon, Shanghai, China). Separation, transfer and immunoblot analysis of proteins was performed as described in previous reports [26,29]. The antibodies of LaminB1 and GAPDH were purchased from Proteintech (Wuhan, China) and AtaGenix (Wuhan, China) respectively.…”

Section: Western Blotmentioning

confidence: 99%

Expressional Localization and Functionally Identifying an RNA Editing Enzyme BmADARa of the Silkworm Bombyx mori

Jiang

Gong

et al. 2020

Insects

Self Cite

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The most common type of RNA editing in metazoans is the deamination of adenosine into inosine (A-to-I) catalyzed by the adenosine deaminase acting on the RNA (ADAR) family of proteins. The deletion or dysfunction of ADAR enzymes in higher eukaryotes can affect the efficiency of substrate editing and cause neurological disorders. However, the information concerning A-to-I RNA editing and ADAR members in the silkworm, Bombyx mori (BmADAR), is limited. In this study, a first molecular comprehensive cloning and sequence analysis of BmADAR transcripts was presented. A complete open reading frame (ORF) (BmADARa) was obtained using RT-PCR and RACE and its expression pattern, subcellular localization and A-to-I RNA-editing function on the silkworm synaptotagmin I (BmSyt I) were investigated. Subcellular localization analysis observed that BmADARa was mainly localized in the nucleus. To further study the A-to-I RNA-editing function of BmADARa, BmSyt I-pIZ-EGFP was constructed and co-transfected with BmADARa-pIZ-EGFP into BmN cells. The result demonstrates that BmADARa can functionally edit the specific site of BmSyt I. Taken together, this study not only provides insight into the function of the first ADAR enzyme in B. mori, but also lays foundations for further exploration of the functional domain of BmADARa and its editing substrates and target sites.

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Haemolymph metabolomic differences in silkworms (Bombyx mori L.) under mulberry leaf and two artificial diet rearing methods

ShanShan

Wang

Liu

et al. 2021

Arch Insect Biochem Physiol

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The new technology of silkworm (Bombyx mori L.) artificial feed breeding has many characteristics and advantages.This study assessed silkworm rearing with mulberry leaf at all instars (MF) as the control, and used metabolomics to explore the differences in haemolymph metabolism of fifth instar silkworms under two modes of rearing with an artificial diet at all instars (AF) and rearing with an artificial diet during first to third instars and mulberry leaf during the fourth and fifth instars (AMF). The results show that, compared with silkworms of the MF group, the amount and fold change of various metabolites were higher in the haemolymph of AF group silkworms, and the metabolism of amino acids and uric acid, carbohydrates, lipids, and vitamins were changed. These changes may be the reasons for the poor performance of the AF silkworms. However, the amount and fold change of the various metabolites of silkworms in the AMF group were lower, and some metabolic pathways were more active. The amount of material and energy supply were greater. These changes could explain the high efficiency growth of body weight of silkworms after the conversion from artificial diet rearing to mulberry leaf rearing. These findings provide an important theoretical basis for the optimisation of artificial diet rearing technology for silkworms.

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BmSUC1 is essential for glycometabolism modulation in the silkworm, Bombyx mori

Cited by 16 publications

References 53 publications

iTRAQ-Based Quantitative Proteomic Analysis of Digestive Juice across the First 48 Hours of the Fifth Instar in Silkworm Larvae

iTRAQ-Based Quantitative Proteomic Analysis of Digestive Juice across the First 48 Hours of the Fifth Instar in Silkworm Larvae

Expressional Localization and Functionally Identifying an RNA Editing Enzyme BmADARa of the Silkworm Bombyx mori

Haemolymph metabolomic differences in silkworms (Bombyx mori L.) under mulberry leaf and two artificial diet rearing methods

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