Boar Seminal Zinc-Precipitable Protein and the Haemagglutinin

Boursnell, J. C.; Noble, E. A.; Andrews, MM

doi:10.1530/jrf.0.0450415

Cited by 4 publications

(4 citation statements)

References 7 publications

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“…3k). These findings are in accordance with those of a previous study indicating that boar vesicular glands might play a key role in the secretion of ZnBPs to the seminal plasma (Boursnell et al, 1975. In bulls, significant amounts of ZnBPs have been detected in the cauda epididymidis and vas deferens (Henkel et al, 2003), whereas in the dog the prostate seems to contribute the bulk of the ZnBPs to the seminal plasma (Mogielnicka-Brzozowska et al, 2012).…”

Section: Resultssupporting

confidence: 92%

“…Using affinity chromatography and electrophoretic techniques, ZnBPs have been isolated and characterized from canine and boar seminal plasma (Mogielnicka-Brzozowska et al, 2011, Mogielnicka-Brzozowska et al, 2012. In the boar ZnBPs have been shown to possess antibacterial, antioxidant and immunomodulating properties , Boursnell et al, 1975, and improved sperm motility and acrosome integrity, when added to extended semen stored at 4ºC (Mogielnicka-Brzozowska et al, 2011). In the literature, the source of ZnBPs of boar seminal plasma has not been clearly defined as yet.…”

Section: Introductionmentioning

confidence: 99%

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The antigenic character of zinc-binding proteins and their localization in boar reproductive tract--a preliminary study.

Mogielnicka-Brzozowska

Fraser

Strzeżek

et al. 2013

Acta Biochim Pol

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In this study immunoelectrophoretic and double immunodiffusion analyses were used to investigate the antigenic character of zinc-binding proteins (ZnBPs), whereas the indirect immunofluorescence technique was used to identify their origin in boar reproductive tract. The mmunoelectrophoretic analysis of ZnBPs of the seminal plasma resulted in the appearance of three antigenic protein complexes, while specific immunoreactivity patterns of the anti-ZnBP serum were detected by double immunodiffusion analysis. Indirect immunofluorescence technique confirmed that ZnBPs were secreted by different reproductive tract tissues, suggesting their contributions to the seminal plasma.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The antigenic character of zinc-binding proteins and their localization in boar reproductive tract--a preliminary study.

Mogielnicka-Brzozowska

Fraser

Strzeżek

et al. 2013

Acta Biochim Pol

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“…Accumulating evidence has been shown that zinc ions, secreted by the prostate and vesicular glands, bind to different proteins ligands, which are implicated in sperm-egg fertilization processes in the female reproductive tract (Boursnell et al 1975, Arver 1982, Hołody and Strzeżek 1999, Siciliano et al 2000, Mogielnicka-Brzozowska et al 2011. Zinc-binding proteins (ZnBPs) have been identified in the seminal plasma of human (Arver 1982, Arver and Eliasson 1982, Siciliano et al 2000 and boar (Boursnell et al 1975, Strzeżek et al 1987, Hołody and Strzeżek 1999, Mogielnicka-Brzozowska et al 2011. Evidence has been shown that most of the zinc in human and canine seminal plasma originates in the prostate (Johnson et al 1969, Lindholmer andGlaumann 1972).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of zinc-binding proteins of canine seminal plasma

Mogielnicka-Brzozowska¹,

Fraser²,

Czarzasta³

et al. 2012

Polish Journal of Veterinary Sciences

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Zinc-binding proteins from seminal plasma (ZnBPs) originate in the secretions of different accessory sex glands and are implicated in key events associated with sperm-egg fertilization processes. This study describes the isolation and characterization of the ZnBPs of canine seminal plasma. Ejaculates were collected from three crossbred dogs for a 2-week period. The ZnBPs as well as non zinc-binding proteins (nZnBPs) were isolated by zinc-dependent affinity chromatography. The isolated fractions were subjected to native gel electrophoresis (one-dimensional polyacryamide gel electrophoresis, PAGE) and sodium dodecyl sulphate polyacryamide gel electrophoresis (SDS-PAGE), using denaturing and reducing conditions. Zinc-elution profile using affinity chromatography displayed two protein fractions represented by the nZnBPs and ZnBPs, respectively. Using native gel electrophoresis, it was found that both the nZnBPs and ZnBPs occurred in their native state as aggregates, ranging from 140 to 669 kDa. The nZnBPs were disaggregated into 8 protein bands, with molecular weights ranging from 10.7 to 79.7 kDa, following SDS-PAGE analysis. By contrast, SDS-PAGE analysis of the ZnBPs revealed 13 protein bands, with molecular weights ranging from 11.6 to 152.3 kDa. Densitometric analysis showed that 46-48% of nZnBPs could be accounted by protein fractions with molecular weights of 10.7 and 14.2 kDa. Also, 2 protein fractions with molecular weights of 11.6 and 14.3 kDa, were predominant in ZnBPs, accounting for approximately 28-30% of the total proteins. These results demonstrate the zinc-binding capacity of proteins secreted by the canine prostate. The findings of this study indicate that ZnBPs of canine seminal plasma comprise several protein fractions, which might be implicated in the reproductive processes in the dog.

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“…The possible involvement of the cationic haemagglutinin present in boar seminal plasma (Boursnell, 1967;Boursnell & Briggs, 1969) was also explored, particularly as it appeared to be capable of binding zinc (Boursnell, Noble & Andrews, 1975). A potent colourless and neutral polyanionic reagent, Antrypol, was used to remove completely the haemagglutinin from the seminal plasma.…”

Section: Introductionmentioning

confidence: 99%

Effect of egg yolk and other reagents upon the zinc of cold-shocked boar spermatozoa

Boursnell¹,

Niemczuk²,

Glos³

1978

Reproduction

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A marked reduction (80.8%) in the zinc uptake by boar spermatozoa cooled to 4 degrees C occurs when the seminal plasma is pretreated with egg yolk-glucose at this temperature. Crude lecithin is less effective (59.8%). Similar pretreatment of the seminal plasma by the polycationic drug Antrypol, which totally removes the zinc-precipitable basic haemagglutinin, does not result in a significant reduction of the sperm zinc uptake at 4 degrees C.

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Boar Seminal Zinc-Precipitable Protein and the Haemagglutinin

Cited by 4 publications

References 7 publications

The antigenic character of zinc-binding proteins and their localization in boar reproductive tract--a preliminary study.

The antigenic character of zinc-binding proteins and their localization in boar reproductive tract--a preliminary study.

Isolation and characterization of zinc-binding proteins of canine seminal plasma

Effect of egg yolk and other reagents upon the zinc of cold-shocked boar spermatozoa

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