Bok-choy promotes growth performance, lipid metabolism and related gene expression in Syrian golden hamsters fed with a high-fat diet

Naseri, Emal; Kong, Xiangyu; Hu, Chunmei; Ayaz, Aliya; Rahmani, Mohammad Malyar; Nasim, Maazullah; Hamdard, Enayatullah; Zahir, Ahmadullah; Zhou, Qian; Wang, Jianjun; Hou, Xilin

doi:10.1039/c9fo02975c

Cited by 5 publications

(5 citation statements)

References 38 publications

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“…Oryzanol is an attractive candidate because of its strong protective effect against metabolic syndrome and its noncytotoxic effect on experimental rodent models. , The results from our studies confirm that oryzanol supplementation significantly attenuated HFCD-induced metabolism disorder, including decreased fat accumulation, serum lipid accumulation, and blood glucose. Other studies report that HFCD led to body fat accumulation and cholesterol metabolism disorder in rodents. , In this study, HFCD treatment increased body weight, fat tissue weight, and serum LDL-c and TG levels, and the LDL-c/HDL-c ratio, all consistent with related studies. , In our previous study, serum LDL-c level of the mice group that received orally administrated oryzanol was reduced by 30% compared to that of the high-fat-diet fed mice group; serum TG levels also decreased significantly in the oryzanol group . Wang et al reported an antihyperlipidemic effect of oryzanol and that the LDL-c/HDL-c ratio decreased by about 22% compared to the ratio in the hyperlipidemia model group .…”

Section: Discussionsupporting

confidence: 91%

“…7,27 In this study, HFCD treatment increased body weight, fat tissue weight, and serum LDL-c and TG levels, and the LDL-c/HDL-c ratio, all consistent with related studies. 28,29 In our previous study, serum LDL-c level of the mice group that received orally administrated oryzanol was reduced by 30% compared to that of the high-fat-diet fed mice group; serum TG levels also decreased significantly in the oryzanol group. 21 Wang et al reported an antihyperlipidemic effect of oryzanol and that the LDL-c/HDL-c ratio decreased by about 22% compared to the ratio in the hyperlipidemia model group.…”

Section: ■ Discussionmentioning

confidence: 90%

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Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism

Yan

Chen

Zhu

et al. 2022

J. Agric. Food Chem.

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Hyperlipidemia is intricately associated with the dysregulation of gut microbiota and host metabolomes. This study explored the antihyperlipidemic function of oryzanol and investigated whether the function of oryzanol affected the gut microbiome and its related metabolites. Hamsters were fed a standard diet (Control) and a high fat and cholesterol (HFCD) diet with or without oryzanol, separately. Our results showed that oryzanol significantly decreased HFCD-induced fat accumulation, serum total cholesterol, low-density lipoprotein cholesterol (LDL-c), LDL-c/HDL-c ratio, triglyceride, and liver steatohepatitis, attenuated HFCD-induced gut microbiota alterations, and altered amino acid concentrations in feces and the liver. We investigated the role of the gut microbiota in the observed beneficial effects; the protective effects of oryzanol were partly diminished by suppressing the gut bacteria of hamsters after using antibiotics. A fecal microbiota transplantation experiment was carried out by transplanting the feces from HFCD group hamsters or hamsters given oryzanol supplementation (as a donor hamster). Our results showed that administering the fecal liquid from oryzanol-treated hamsters attenuated HFCD-induced hyperlipidemia, significantly decreased the abundance of norank_f__Erysipelotrichaceae, norank_f__Eubacteriaceae, and norank_f__Oscillospiraceae and the concentration of tyrosine. These outcomes are significantly positively correlated with serum lipid concentration. This study illustrated that gut microbiota is the target of oryzanol in the antihyperlipidemic effect.

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Section: Discussionsupporting

confidence: 91%

Section: ■ Discussionmentioning

confidence: 90%

Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism

Yan

Chen

Zhu

et al. 2022

J. Agric. Food Chem.

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“…The decreased level of LPL blocks the lipid metabolism and helps the accumulation of fat in the liver ( 38 ). Several studies showed the increasing levels of FASN and CEBP- α in the high-fat diet compared with the control group ( 39 , 40 ). The reduced level of HMG-CoA participates in the reduction of cholesterol synthesis, which is associated with lipid metabolism, whereas PPAR- α and CPT2 genes are associated with fatty acid β-oxidation ( 41 ).…”

Section: Discussionmentioning

confidence: 99%

Diets Partially Replaced With Cassava Residue Modulate Antioxidant Capacity, Lipid Metabolism, and Gut Barrier Function of Huanjiang Mini-Pigs

Azad

Jiang

et al. 2022

Front. Vet. Sci.

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Agricultural by-products have been identified as potential feed resources in animal production. The present study investigated the effects of cassava residue (CR) or fermented CR (FCR) on antioxidant capacity, immunity, gut barrier functions, and lipid metabolism in pigs. A total of 120 healthy Huanjiang mini-piglets were assigned into three groups, including control group (basal diet), CR group (basal diet + 5% CR), and FCR group (basal diet + 5% FCR). The experiment lasted for 30 days. The results showed that, dietary CR or FCR supplementation increased the jejunal catalase (CAT, P = 0.063) and glutathione peroxidase (GSH-Px, P < 0.05) levels and hepatic superoxide dismutase (SOD, P < 0.05) level while decreased (P = 0.077) ileal malondialdehyde (MDA) level, when compared with the control group. Dietary CR supplementation increased intestinal SOD and hepatic GSH-Px levels, whereas decreased jejunal and hepatic MDA levels (P < 0.05). Dietary CR supplementation increased the levels of secretory immunoglobulin A (sIgA) in the intestine and liver, as well as jejunal interleukin (IL)-10, ileal tumor necrosis factor (TNF)-α, and hepatic interferon (IFN)-γ, whereas dietary CR or FCR supplementation decreased the jejunal IL-1β level and increased hepatic IL-10 level (P < 0.05). In the intestinal microbiota analysis, dietary CR or FCR supplementation enhanced the colonic α-diversity and ileal Actinobacteria abundance, whereas decreased ileal Verrucomicrobia and colonic Tenericutes abundances (P < 0.05). In addition, dietary FCR supplementation increased Firmicutes and decreased Bacteroidetes abundances in the ileum and colon, whereas CR supplementation increased Escherichia-Shigella and decreased Terisporobacter abundances in the ileum (P < 0.05). Moreover, dietary CR or FCR supplementation up-regulated (P < 0.05) the gene expressions related to gut barrier functions of piglets. However, dietary CR supplementation showed negative impacts on hepatic lipid metabolism by up-regulating the expression of genes associated with fatty acid synthesis and triglyceride and lipid metabolism. In conclusion, dietary CR or FCR supplementation can maintain the health of piglets by increasing antioxidant capacity, gut barrier function, and altering the intestinal microbiota composition, but CR supplementation may increase the potential risk of abnormal lipid metabolism.

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“…These data demonstrate that we have established a well-high lipid model in Golden Syrian hamsters. We don’t use negative control instead of using self-control when feeding with HFD because there were several reports claiming that more than 1.3-fold changes in serum TC, TG, and LDL are enough to establish a well-high lipid model [ 14 , 15 ].…”

Section: Resultsmentioning

confidence: 99%

Comparison of protective effects of hesperetin and pectolinarigenin on high-fat diet-induced hyperlipidemia and hepatic steatosis in Golden Syrian hamsters

et al. 2023

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A comparative study was conducted to determine whether hesperetin and pectolinarigenin could lower total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL), and high-density lipoprotein cholesterol (HDL) in a high-fat diet (HFD)-induced high lipid model in Golden Syrian hamsters. 48 Golden Syrian hamsters (8 weeks old) were fed with a HFD for 15 days. HFD induced significant increases in plasma TC, TG, LDL, and HDL. Then, these high lipid hamsters were divided into four groups and were administered with 0.5% sodium carboxymethyl cellulose (CMC-Na), hesperetin (100 mg/kg/day), pectolinarigenin (100 mg/kg/day) or atorvastatin (1.0 mg/kg/day), for 7 weeks. It was found that pectolinarigenin treatment resulted in significant reductions in body weight, adiposity index, serum levels of TC, TG and hepatic TC, TG and free fatty acid compared to those in control hamsters with hyperlipidemia (P<0.05). However, hesperetin treatment only caused reductions in plasma TC and hepatic TG levels. Besides, the hamsters treated with pectolinarigenin showed a relatively normal hepatic architecture compared to the hepatic steatosis shown in the control group. Moreover, the expressions of fatty-acid synthase (Fasn) and solute carrier family 27 member 1 (Slc27a1) involved in lipid biosynthesis, were suppressed in the pectolinarigenin-treated groups, and the expression of carnitine palmitoyltransferase 1A (Cpt1a) involved in fatty acid oxidation was increased in the pectolinarigenin-treated group. Taken together, these results suggest pectolinarigenin exerts stronger protective effects against hyperlipidemia and hepatic steatosis than hesperetin, which may involve the inhibition of lipid uptake and biosynthesis.

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Bok-choy promotes growth performance, lipid metabolism and related gene expression in Syrian golden hamsters fed with a high-fat diet

Abstract: This study aims to investigate the beneficial effects of two cultivars of bok-choy, ‘Suzhouqing’ (green cultivar) and ‘Ziluolan’ (purple cultivar), on growth performance, lipid metabolism and related gene expressions in Syrian golden hamsters.

Cited by 5 publications

References 38 publications

Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism

Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism

Diets Partially Replaced With Cassava Residue Modulate Antioxidant Capacity, Lipid Metabolism, and Gut Barrier Function of Huanjiang Mini-Pigs

Comparison of protective effects of hesperetin and pectolinarigenin on high-fat diet-induced hyperlipidemia and hepatic steatosis in Golden Syrian hamsters

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