Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactors

Marolt, Darja; Augst, Alexander; Freed, Lisa E.; Vepari, Charu; Fajardo, Robert; Patel, Nipun; Gray, Martha L.; Farley, Michelle; Kaplan, David L.; Vunjak-Novakovic, Gordana

doi:10.1016/j.biomaterials.2006.07.015

Cited by 173 publications

(148 citation statements)

References 71 publications

(132 reference statements)

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“…In these cases, the goal is to produce a neo-formed tissue in vitro for later implantation in vivo. 70,71 Regardless of the method used to regenerate diseased or damaged musculoskeletal tissue, a common element in all of these cases is the use of inductive factors. Cells that are implanted either directly into the defect or first within a scaffold, as well as cells from the host that are recruited to the site of injury, all require stimulation by growth factors or morphogens to help in the formation of newly formed tissue.…”

Section: Discussionmentioning

confidence: 99%

Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

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Regenerative medicine holds great promise for orthopaedic surgery. As surgeons continue to face challenges regarding the healing of diseased or injured musculoskeletal tissues, regenerative medicine aims to develop novel therapies that will replace, repair, or promote tissue regeneration. This review article will provide an overview of the different research areas involved in regenerative medicine, such as stem cells, bioinductive factors, and scaffolds. The potential use of stem cells for orthopaedic tissue engineering will be addressed by presenting the current progress with skeletal muscle-derived stem cells. As well, the development of a revascularized massive allograft will be described and will serve as a prototypic model of orthopaedic tissue engineering. Lastly, we will describe current approaches used to design cell instructive materials and how they can be used to promote and regulate the formation of bony tissue. ß

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Section: Discussionmentioning

confidence: 99%

Regenerative medicine in orthopaedic surgery

Corsi

Schwarz

Mooney

et al. 2007

Journal Orthopaedic Research

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“…We selected the cell source (hMSCs), scaffold type (porous silk) and bioreactor system (rotating vessels) based on our previous studies of tissue-engineered cartilage-and bone-like tissues. In these studies, hMSCs were shown to differentiate into chondrogenic and osteogenic lineages when cultured on silk scaffolds and subjected to chondrogenic or osteogenic regulatory factors (Meinel et al 2004a-c;Kim et al 2005a;Wang et al 2005;Hofmann et al 2006;Marolt et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors

Augst

Marolt

Freed

et al. 2008

J. R. Soc. Interface.

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Human mesenchymal stem cells (hMSCs) isolated from bone marrow aspirates were cultured on silk scaffolds in rotating bioreactors for three weeks with either chondrogenic or osteogenic medium supplements to engineer cartilage-or bone-like tissue constructs. Osteochondral composites formed from these cartilage and bone constructs were cultured for an additional three weeks in culture medium that was supplemented with chondrogenic factors, supplemented with osteogenic factors or unsupplemented. Progression of cartilage and bone formation and the integration between the two regions were assessed by medical imaging (magnetic resonance imaging and micro-computerized tomography imaging), and by biochemical, histological and mechanical assays. During composite culture (three to six weeks), bone-like tissue formation progressed in all three media to a markedly larger extent than cartilage-like tissue formation. The integration of the constructs was most enhanced in composites cultured in chondrogenic medium. The results suggest that tissue composites with well-mineralized regions and substantially less developed cartilage regions can be generated in vitro by culturing hMSCs on silk scaffolds in bioreactors, that hMSCs have markedly higher capacity for producing engineered bone than engineered cartilage, and that chondrogenic factors play major roles at early stages of bone formation by hMSCs and in the integration of the two tissue constructs into a tissue composite.

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“…Yoshioka and colleagues [32] reported successful regeneration of cartilage in osteochondral-defective Japanese white rabbits using cartilaginous constructs obtained from culturing BMSCs. Yoshioka and colleagues culturing human BMSCs on a silk scaffold for 5 weeks produced 8 mm diameter by 2 mm thick cartilaginous constructs [33].…”

Section: Rotating Wall Vesselmentioning

confidence: 99%