Bone Angiogenesis and Vascular Niche Remodeling in Stress, Aging, and Diseases

Stucker, Sina; Chen, Junyu; Watt, Fiona E.; Kusumbe, Anjali P.

doi:10.3389/fcell.2020.602269

Cited by 42 publications

(39 citation statements)

References 248 publications

(388 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Osteoporosis has become a major public problem with a signi cant population burden, especially in the aging population [24,25] . Overactivation of bone resorption plays a critical role in the pathological mechanisms of osteoporosis [26] . Until now, osteoclasts were known large multinucleated cells with exclusive bone-resorbing ability [27] .…”

Section: Discussionmentioning

confidence: 99%

Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways

Jin

Yang

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Osteoclast cell increase is a major risk factor for osteoporosis and degenerative bone and joint diseases. Thioacetamide (TAA) can lead to many types of liver and kidney damage, but less attention has been paid to the association of TAA with bone damage. In this work, we investigated the effects of TAA on the osteoclastogenesis and differentiation of bone marrow macrophages (BMMs). Methods Bone marrow mononuclear macrophages of SD rat suckling mice were taken for primary culture. CCK-8 was used to detect the toxic effects of TAA on BMMs, and flow cytometry was used to detect the effects of TAA on the cell cycle, cell viability, apoptosis and intracytoplasmic Ca2+ concentration of BMMs. TRAP immunohistochemistry and fluorescence staining were used to detect the effect of TAA on osteoclast differentiation of BMMs. Western Blot was used to detect the expression level of PI3K/AKT pathway and osteoclast-specific proteins (TRAP and Cts-K). Results The results suggested that TAA inhibited the proliferation of BMMs, while enhancing osteoclastogenesis at 0.5 mg/mL and 1 mg/mL as assayed by TRAP staining, and BMMs could differentiate into osteoclast-like cells with overexpression of cathepsin K and TRAP proteins after TAA treatment. Western blot results showed that TAA can activate the expression levels of P-PI3K, P-AKT, P-P38, and P-JNK, accompanied by apoptosis of BMM cells and increase of intracellular Ca2+. Conclusion TAA may induce osteoclast formation in BMMs by activating the expression of PI3K/AKT pathway proteins, which is comparable to the classic osteoclast differentiation inducer RANKL. This suggests that we may find a cheap osteoclast inducer.

show abstract

Section: Discussionmentioning

confidence: 99%

Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways

Jin

Yang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…A direct connection of aging hematopoiesis with age-related changes in bone and cartilaginous tissues has been established [155]. Moreover, it has become clear that the aging of bones has an earlier onset than the age-related decline in the hematopoietic and immune systems, confirming the idea that changes in bones and bone marrow may be the basis on which functional reduction of HSCs occurs [104].…”

Section: Mesenchymal Cells During Agingmentioning

confidence: 92%

Hematopoiesis during Ontogenesis, Adult Life, and Aging

Belyavsky

Petinati

2021

IJMS

View full text Add to dashboard Cite

In the bone marrow of vertebrates, two types of stem cells coexist—hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs). Hematopoiesis only occurs when these two stem cell types and their descendants interact. The descendants of HSCs supply the body with all the mature blood cells, while MSCs give rise to stromal cells that form a niche for HSCs and regulate the process of hematopoiesis. The studies of hematopoiesis were initially based on morphological observations, later extended by the use of physiological methods, and were subsequently augmented by massive application of sophisticated molecular techniques. The combination of these methods produced a wealth of new data on the organization and functional features of hematopoiesis in the ontogenesis of mammals and humans. This review summarizes the current views on hematopoiesis in mice and humans, discusses the development of blood elements and hematopoiesis in the embryo, and describes how the hematopoietic system works in the adult organism and how it changes during aging.

show abstract

“…The vasculature of the skeletal system is crucial for delivering nutrients and oxygen to the stem and progenitor cells that reside in specialized vascular niches in the bone marrow (BM). Moreover, BM ECs secrete a variety of angiocrine signals to maintain resident stem cells and regulate bone angiogenesis, osteogenesis and hematopoiesis ( Colmone and Sipkins, 2008 ; Sivan et al, 2019 ; Stucker et al, 2020 ). BM ECs show a remarkable heterogeneity based on the distinct expression pattern of vascular cell surface markers such as CD31 (Pecam-1), Endomucin and E-selectin ( Winkler et al, 2012 ; Kusumbe et al, 2014 ).…”

Section: Bone: Vascular Heterogeneity Aging and Endocrine Functionsmentioning

confidence: 99%

“…Skeletal aging induces significant morphological changes in BM endothelium, including decreased vascular integrity and increased leakiness ( Poulos et al, 2017 ; Stucker et al, 2020 ). Aged BM exhibits a significant reduction in arteriolar and type H vessels as well as PDGFRβ-expressing pericytes ( Kusumbe et al, 2014 , 2016 ; Singh et al, 2019 ).…”

Section: Bone: Vascular Heterogeneity Aging and Endocrine Functionsmentioning

confidence: 99%

Heterogeneity and Dynamics of Vasculature in the Endocrine System During Aging and Disease

2021

Self Cite

View full text Add to dashboard Cite

The endocrine system consists of several highly vascularized glands that produce and secrete hormones to maintain body homeostasis and regulate a range of bodily functions and processes, including growth, metabolism and development. The dense and highly vascularized capillary network functions as the main transport system for hormones and regulatory factors to enable efficient endocrine function. The specialized capillary types provide the microenvironments to support stem and progenitor cells, by regulating their survival, maintenance and differentiation. Moreover, the vasculature interacts with endocrine cells supporting their endocrine function. However, the structure and niche function of vasculature in endocrine tissues remain poorly understood. Aging and endocrine disorders are associated with vascular perturbations. Understanding the cellular and molecular cues driving the disease, and age-related vascular perturbations hold potential to manage or even treat endocrine disorders and comorbidities associated with aging. This review aims to describe the structure and niche functions of the vasculature in various endocrine glands and define the vascular changes in aging and endocrine disorders.

show abstract

Bone Angiogenesis and Vascular Niche Remodeling in Stress, Aging, and Diseases

Cited by 42 publications

References 248 publications

Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways

Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways

Hematopoiesis during Ontogenesis, Adult Life, and Aging

Heterogeneity and Dynamics of Vasculature in the Endocrine System During Aging and Disease

Contact Info

Product

Resources

About