Bone Healing Capacity of Demineralized Dentin Matrix Materials in a Mini-pig Cranium Defect

Kim, Jong-Yub; Kim, Kyung-Wook; Um, In-Woong; Kim, Young-Kyun; Lee, Jeong Keun

doi:10.5856/jkds.2012.5.1.21

Cited by 17 publications

(15 citation statements)

References 22 publications

(27 reference statements)

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“…Osteonectin is considered as a biomarker for the onset of crystal nucleation, and is involved in binding Ca 2+ ions and hydroxyapatite during mineralization 30,35,36) . In concordance with our results, a study reported higher active expression of osteonectin with DDM in a mini-pig cranium defect model at 4 and 12 weeks compared to the control after grafting 37) . The present study focused only on the initial stages of osteogenesis, i.e., up to 48 h 36,38) .…”

Section: Discussionsupporting

confidence: 93%

Osteogenic Potential of Demineralized Dentin Matrix as Bone Graft Material

Hyo-Jung

Hong

Kim

et al. 2017

J. Hard Tissue Biology.

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The aim of this study was to evaluate the osteogenic potential of demineralized dentin matrix (DDM) in supporting the osteogenic activity of MG-63 cells and compare it to a mixture of inorganic bovine bone and collagen (Bio-Oss Collagen ® ). Cell morphology, differentiation, adhesion, and growth were investigated via scanning electron microscopy and immunofluorescence analyses of F-actin, osteocalcin, and osteonectin. Cell adhesion and growth on the DDM as a bone graft material were more abundant, with cells adopting a flat shape and uniform distribution, than on the Bio-Oss Collagen ® at all the observation time points (i.e., 6, 12, 24, 48, 72 h and 14 days). Cell attachment and cytoskeleton organization assessed using confocal laser scanning microscopy revealed that the DDM provided better attachment with cytoplasmic propagation than the control. Immunofluorescence assays showed that the fluorescent intensities of osteocalcin and osteonectin as biomarkers of cellular differentiation were higher on the DDM than on Bio-Oss Collagen ® at 6, 12 and 24 h. We showed that the DDM had an enhanced osteogenic potential as a bone substitute for bone augmentation procedures in the dental and medical fields.

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Section: Discussionsupporting

confidence: 93%

Osteogenic Potential of Demineralized Dentin Matrix as Bone Graft Material

Hyo-Jung

Hong

Kim

et al. 2017

J. Hard Tissue Biology.

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“…Extensive studies have been conducted on DDM with its osteoinductivity and osteoconductivity in vitro and in vivo [17,18,[48][49][50][51]. In animal studies, DDM enhanced the bone healing process of tooth sockets, and promoted new bone formation in rat calvaria defects, rabbit parietal defects, minipig crania defects, sheep iliac defects, and porcine sinus [17,18,41,[47][48][49]52]. Besides bone, DDM was employed in osteochondral regeneration [20].…”

Section: Application Of Ddm In Bone Regenerationmentioning

confidence: 99%

Nano-Structured Demineralized Human Dentin Matrix to Enhance Bone and Dental Repair and Regeneration

et al. 2019

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Demineralized dentin matrix (DDM), derived from human teeth, is an excellent scaffold material with exciting bioactive properties to enhance bone and dental tissue engineering efficacy. In this article, first the nano-structure and bioactive components of the dentin matrix were reviewed. Then the preparation methods of DDM and the resulting properties were discussed. Next, the efficacy of DDM as a bone substitute with in vitro and in vivo properties were analyzed. In addition, the applications of DDM in tooth regeneration with promising results were described, and the drawbacks and future research needs were also discussed. With the extraction of growth factors from DDM and the nano-structural properties of DDM, previous studies also broadened the use of DDM as a bioactive carrier for growth factor delivery. In addition, due to its excellent physical and biological properties, DDM was also investigated for incorporation into other biomaterials design and fabrication, yielding great enhancements in hard tissue regeneration efficacy.

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“…In other words, DDM has inorganic components, which is the most smilar to autogenous bone, and includes organic components engaged in osteoinduction. DDM has microporous structures named dentinal tubules that could be loaded with bone growth factors such as BMP 15,[17][18][19][20] . In other words, it is con-sidered DDM can play the role of excellent bone graft material and also of carrier of growth factors.…”

Section: Discussionmentioning

confidence: 99%