Bone inductive proteins to enhance postorthodontic stability

Abstract

Objectives: To evaluate the use of bone morphogenetic proteins to enhance postorthodontic stability in sheep and to develop a biological method of postorthodontic retention. Materials and Methods: First incisors were extracted in four mature and healthy sheep, and the second incisors were tipped reciprocally toward the midline and then retained. Dried bone matrix was injected into the distal periodontal space of the left second incisor. The right second incisor was left as a control. Both incisors were retained in the tipped position for 4 weeks. Then, the orthodontic appliance was removed and the teeth were left without retention. Six weeks later, the animals were killed and serial sections were prepared for histologic observation. Results: Unlike the control, the experimental second incisor maintained its tipped position with minimal relapse. On the distal periodontal space of the experimental tooth, areas of focal fusion between newly formed bone and newly formed areas of hypercementosis were observed. In the distal periodontal space of the control tooth, osteoclastic activity was observed along most of the socket wall, and the periodontal space appeared narrow and compressed. This brought the tooth close to the boundary of the alveolar bone, confirming the relapse observed on that side. Conclusion: This study proposes a new method of retention in which a biologically safe osteoinductive material is used to retain the teeth via induction of points of approximation between the cementum and alveolar bone.

Enhancement of post‐orthodontic tooth stability by carbonated hydroxyapatite‐incorporated advanced platelet‐rich fibrin in rabbits

“…Moreover, the relapse rate in the CHA‐aPRF group was significantly lower than in the other groups, and this was associated with a significant decrease in osteoclast activity (TRAP‐positive cells), with a concomitant significant increase in osteoblast number. This finding indicates that differences in the appearance of osteoclast‐osteoblast may affect the relapse movement and this was in accordance with the results of Hassan et al The decreasing number of TRAP‐positive cells, indicates decreased resorption, and thus, decreased OTM. The use of TRAP as a marker for osteoclast activity has been well‐documented .…”

The effect of platelet rich plasma injection on relapse of orthodontically moved teeth in rabbits

“…This might suggest that PRP injection speeded the remodeling of the PDL and promoted new bone formation at a higher rate helping in faster stabilization of the new tooth position. These findings comes in accordance with Hassan AH, Al-Hubail A and Al-Fraidi AA (13) who injected bone morphogenic proteins (BMPs) Volume 51 -June 2017 around orthodontically moved teeth and Han G et al (12) after systemic administration of simvistatin following removal of orthodontic forces. Both (BMPs) and simvistatin are bone inductive materials.…”

“…This randomized controlled animal study was carried out to test the hypothesis that platelet rich plasma (PRP) can be used as a safe biological retainer to minimize or prevent tooth relapse following orthodontic tooth movement. The literature reported some successful attempts for biological control of relapse using pharmacological drugs (9)(10)(11)(12)(13)(14) . However, the use of PRP has superior advantages over any other pharmacological drug.…”

“…The orthodontic literature shows successful biological control of orthodontic relapse by pharmacological agents either by inhibition of osteoclastic activity (9)(10)(11) or by promoting osteoblasts function (12)(13)(14) .…”

Controlled release of injectable liposomalin situgel loaded with recombinant human bone morphogenetic protein-2 for the repair of alveolar bone clefts in rabbits

“…Previous studies have shown the ability of BMPs to induce bone formation in a variety of models having many clinical applications in orthopaedics and oral and maxillofacial surgery (Geiger et al, 2003;Hassan et al, 2010;Herford & Boyne, 2008;Urist et al, 1984). Delivery systems for biosafe growth factors and biodegradable carriers have been used to achieve the slow, controlled release of growth factors at the site of implantation (Herford & Boyne, 2008).…”