Bone marrow biopsy in adult acute lymphoblastic leukemia: morphological characteristics and contribution to the study of prognostic factors

Splenic red pulp lymphoma with numerous basophilic villous lymphocytes: a distinct clinicopathologic and molecular entity?

“…Six BM biopsies were processed into plastic according to the methods previously described. 12 Briefly, undecalcified BM specimens were fixed in phosphate-buffered saline-buffered formalin, dehydrated in acetone, and embedded in a Kushida low-viscosity glycol-methacrylate Immuno-Bed resin (Polysciences Europe, Eppelheim, Germany). Twomicrometer sections were stained by usual histologic procedures using Giemsa and eosin panoptic staining for cytologic details and Gomori silver impregnation to show reticulin fibers.…”

Bone marrow fibrosis in childhood acute lymphoblastic leukemia correlates to biological factors, treatment response and outcome

“…[10][11][12] Wallis and Reid 13 found a significant association between the presence of BM fibrosis and B-cell lineage in childhood ALL. Thomas et al 14 confirmed this association in adult B-cell lineage ALL, but found no prognostic significance.…”

Rethinking the usefulness of bone marrow biopsy on treatment decision in CLL patients at diagnosis

“…Unlike PMF, BMRF is not a hallmark of myelodysplastic syndrome (MDS) and acute leukemias. However, increased BMRF was associated with more severe disease and poor survival in MDS patients, similar to PMF patients [18][19][20], whereas BMRF could not retain such a prognostic interest in adult leukemia patients [21,27]. In this study, we found that advanced BMRF was associated with only Binet stage regarding the severity of disease.…”

“…Although BMIP described as interstitial, nodular, mixed, and diffuse, is particularly used in CLL [3,[5][6][7], the amount of bone marrow reticulin has been delineated in many diseases [8], and BMRF has been shown to be associated with various benign [24] and malignant hematological [8][9][10][11][12][13][14][15][16][17][18][19][20][21] diseases. Nevertheless, the significance of BMRF has not been yet well defined as BMIP in CLL.…”

“…Bone marrow infiltration pattern may occur as interstitial, nodular, mixed (nodular and interstitial), or diffuse pattern [3,[5][6][7], and diffuse BMIP has been defined among the poor prognostic factors for CLL [3,5,6]. Besides BMIP, bone marrow biopsy examination usually comprises information about the presence of bone marrow reticulin fibrosis (BMRF) whose effect has been studied in a number of hematological malignant diseases [8][9][10][11][12][13][14][15][16][17][18][19][20][21]. The prognostic impact of BMRF was also studied in CLL patients, showing the negative implication of advanced BMRF [22].…”