Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion

Fernandes, Marcela; Valente, Sandra Gomes; Fernandes, María José da Silva; Félix, Evandro Penteado Villar; Naffah-Mazzacoratti, Maria da Graça; Amado, Débora; Santos, João Baptista Gomes dos; Leite, Vilnei Mattioli; Faloppa, Flávio

doi:10.1016/j.jneumeth.2007.12.009

Cited by 28 publications

(30 citation statements)

References 24 publications

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“…Reviewing the published results of 10 mm autograft, the SFI on Day 60 were respectively −79.9 and -76.74 [17], [18]. None of the repair methods including nerve autografting were able to restore SFI parameters to control levels [18].…”

Section: Discussionmentioning

confidence: 97%

The Effects of Irreversible Electroporation (IRE) on Nerves

et al. 2011

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BackgroundIf a critical nerve is circumferentially involved with tumor, radical surgery intended to cure the cancer must sacrifice the nerve. Loss of critical nerves may lead to serious consequences. In spite of the impressive technical advancements in nerve reconstruction, complete recovery and normalization of nerve function is difficult to achieve. Though irreversible electroporation (IRE) might be a promising choice to treat tumors near or involved critical nerve, the pathophysiology of the nerve after IRE treatment has not be clearly defined.MethodsWe applied IRE directly to a rat sciatic nerve to study the long term effects of IRE on the nerve. A sequence of 10 square pulses of 3800 V/cm, each 100 µs long was applied directly to rat sciatic nerves. In each animal of group I (IRE) the procedure was applied to produce a treated length of about 10 mm. In each animal of group II (Control) the electrodes were only applied directly on the sciatic nerve for the same time. Electrophysiological, histological, and functional studies were performed on immediately after and 3 days, 1 week, 3, 5, 7 and 10 weeks following surgery.FindingsElectrophysiological, histological, and functional results show the nerve treated with IRE can attain full recovery after 7 weeks.ConclusionThis finding is indicative of the preservation of nerve involving malignant tumors with respect to the application of IRE pulses to ablate tumors completely. In summary, IRE may be a promising treatment tool for any tumor involving nerves.

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Section: Discussionmentioning

confidence: 97%

The Effects of Irreversible Electroporation (IRE) on Nerves

et al. 2011

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“…Furthermore, the mononuclear fraction is easy to obtain, implicating in lower costs and less processing time, as compared with other cell types [1], [29].…”

Section: Discussionmentioning

confidence: 99%

Influence of Delivery Method on Neuroprotection by Bone Marrow Mononuclear Cell Therapy following Ventral Root Reimplantation with Fibrin Sealant

et al. 2014

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The present work compared the local injection of mononuclear cells to the spinal cord lateral funiculus with the alternative approach of local delivery with fibrin sealant after ventral root avulsion (VRA) and reimplantation. For that, female adult Lewis rats were divided into the following groups: avulsion only, reimplantation with fibrin sealant; root repair with fibrin sealant associated with mononuclear cells; and repair with fibrin sealant and injected mononuclear cells. Cell therapy resulted in greater survival of spinal motoneurons up to four weeks post-surgery, especially when mononuclear cells were added to the fibrin glue. Injection of mononuclear cells to the lateral funiculus yield similar results to the reimplantation alone. Additionally, mononuclear cells added to the fibrin glue increased neurotrophic factor gene transcript levels in the spinal cord ventral horn. Regarding the motor recovery, evaluated by the functional peroneal index, as well as the paw print pressure, cell treated rats performed equally well as compared to reimplanted only animals, and significantly better than the avulsion only subjects. The results herein demonstrate that mononuclear cells therapy is neuroprotective by increasing levels of brain derived neurotrophic factor (BDNF) and glial derived neurotrophic factor (GDNF). Moreover, the use of fibrin sealant mononuclear cells delivery approach gave the best and more long lasting results.

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“…Following surgery, each animal was given an injection of fentanyl (0.032 mg/kg), administered intraperitoneally for infection prophylaxis and analgesia. Analgesic was then administered at 12-h intervals for 2 consecutive days [12].…”

Section: Surgery Proceduresmentioning

confidence: 99%

Low-level laser therapy improves repair following complete resection of the sciatic nerve in rats

et al. 2011

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The aim of this study is to analyze the effects of low-level laser therapy (LLLT) on the regeneration of the sciatic nerve in rats following a complete nerve resection. Male Wistar rats were divided into a control injury group, injury groups irradiated with a 660-nm laser at 10 or 50 J/cm(2), and injury groups irradiated with an 808-nm laser at 10 or 50 J/cm(2). Treatment began 24 h following nerve resection and continued for 15 days. Using the sciatic functional index (SFI), we show that the injured animals treated with 660 nm at 10 and 50 J/cm(2) had better SFI values compared with the control injury and the 808-nm groups. Animals irradiated with the 808-nm laser at 50 J/cm(2) show higher values for fiber density than do control animals. In addition, axon and fiber diameters were larger in animals irradiated with 660 nm at 50 J/cm(2) compared to the control group. These findings indicate that 660-nm LLLT is able to provide functional gait recovery and leads to increases in fiber diameter following sciatic nerve resection.

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Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion

Cited by 28 publications

References 24 publications

The Effects of Irreversible Electroporation (IRE) on Nerves

The Effects of Irreversible Electroporation (IRE) on Nerves

Influence of Delivery Method on Neuroprotection by Bone Marrow Mononuclear Cell Therapy following Ventral Root Reimplantation with Fibrin Sealant

Low-level laser therapy improves repair following complete resection of the sciatic nerve in rats

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