Bone marrow‐derived mesenchymal stem cells differentiation into tubular epithelial‐like cells in vitro

Abstract

The possibility of differentiating bone marrow-derived mesenchymal stem cells (BMSCs) into tubular epithelial-like cells is explored in vitro. Purified BMSCs from Sprague-Dawley rats were obtained by density gradient centrifugation. Third generation BMSCs were divided into six groups and were cultured under different conditions. The expression of alkaline phosphatase and cytokeratin (CK)-18 protein was detected through staining and immunocytochemistry, respectively, and the expression of E-cadherin proteins was recorded through immunofluorescence. Some cells in ischemia/reperfusion (I/R), all-trans retinoic acid (ATRA), epidermal growth factor (EGF) and bone morphogenetic protein-7 (BMP-7) groups turned positive, whereas the positive cells in the combined group significantly increased compared with the other groups. Compared with the control group, the positive expression rates of CK-18 in the I/R, ATRA, EGF, BMP-7 and the combined group were 11·50% ± 3·84%, 27·40% ± 2·70%, 29·60% ± 4·51%, 26·80% ± 5·00% and 44·00% ± 3·16%, respectively, and CK-18 mRNA expression in the combined group was obviously higher than that in the other groups (P < 0·01). Immunofluorescence detection showed that E-cadherin expression was not detectable in the control group, whereas the positive expression rates of E-cadherin in the I/R, ATRA, EGF, BMP-7 and the combined group were 6·75% ± 2·13%, 16·40% ± 2·69%, 18·25% ± 3·50%, 16·06% ± 2·00% and 30·26% ± 5·16%, respectively. The addition of ATRA, EGF and BMP-7 induces BMSCs differentiation into tubular epithelial-like cells in stimulated acute renal failure microenvironment in vitro.

In vitro expression of cytokeratin 18, 19 and tube formation of adipose‐derived stem cells induced by the breast epithelial cell line HBL‐100

“…Therefore, the observed positive expression of these two markers can indicate an epithelial differentiation 21, 22.…”

Generating CK19-Positive Cells with Hair-Like Structures from Wharton's Jelly Mesenchymal Stromal Cells

“…For example, Baer et al generated epithelial cells from adipose-derived adult MSCs [12]. Wan et al also reported the generation of epithelial cells from bone marrow-derived MSCs [13].…”

“…In some areas of spheroids, we observed that these cells were close to hair-like structures, an association that led to the conclusion that the generated CK19-positive cells might reflect hair follicle cells. Generating epithelial cells from mesenchymal cells has been recently reported [10][11][12][13]. For example, Baer et al generated epithelial cells from adipose-derived adult MSCs [12].…”

“…With the exception of neural cells, these tissues are mesodermal in origin. Generating epithelial cells from mesenchymal cells has been recently reported [10][11][12][13][14]. Since differentiation into cells of all somatic lineages is a feature of pluripotent stem cells, like embryonic stem cells [15], differentiation of MSCs into cells of mesodermal or ectodermal origin supports the hypothesis that mesenchymal stromal cells are pluripotent.…”

Células madre Mesenquimales de tejido adiposo como tratamiento de la superficie ocular en la deficiencia de células madre limbares

“…Existen publicaciones que han demostrado que las MSCs pueden diferenciarse in vitro hacia determinadas estirpes de células epiteliales mediante la realización de co-cultivos celulares y/o mediante la suplementación de los medios de cultivo con determinados cócteles de factores de crecimiento concretos como los factores de crecimiento epitelial (EGF), de queratinocitos (KGF) y de hepatocitos (HGF), y/o otros suplementos. [108][109][110][111][112] Además, se ha demostrado que las MSCs pueden contribuir a la regeneración de tejidos epiteliales tales como el epitelio bronquial y alveolar, 113,114 el epitelio glomerular y de los túbulos renales, 115,116 el epitelio gastrointestinal, 117 el epitelio pigmentario de la retina 118,119 y el epitelio corneal, 120 entre otros.…”