Generating CK19-Positive Cells with Hair-Like Structures from Wharton's Jelly Mesenchymal Stromal Cells

Aljitawi, Omar S.; Xiao, Yinghua; Zhang, Da; Stehno‐Bittel, Lisa; Garimella, Rama Murthy; Hopkins, Richard A.; Detamore, Michael S.

doi:10.1089/scd.2012.0184

Cited by 12 publications

(6 citation statements)

References 25 publications

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“…The decellularization procedure has recently been described in our earlier publication [ 13 ]. Briefly, fresh human umbilical cords were transported from the delivery room in a transport solution at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications

et al. 2017

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In tissue engineering, an ideal scaffold attracts and supports cells thus providing them with the necessary mechanical support and architecture as they reconstruct new tissue in vitro and in vivo. This manuscript details a novel matrix derived from decellularized Wharton’s jelly (WJ) obtained from human umbilical cord for use as a scaffold for tissue engineering application. This decellularized Wharton’s jelly matrix (DWJM) contained 0.66 ± 0.12 μg/mg sulfated glycosaminoglycans (GAGs), and was abundant in hyaluronic acid, and completely devoid of cells. Mass spectroscopy revealed the presence of collagen types II, VI and XII, fibronectin-I, and lumican I. When seeded onto DWJM, WJ mesenchymal stem cells (WJMSCs), successfully attached to, and penetrated the porous matrix resulting in a slower rate of cell proliferation. Gene expression analysis of WJ and bone marrow (BM) MSCs cultured on DWJM demonstrated decreased expression of proliferation genes with no clear pattern of differentiation. When this matrix was implanted into a murine calvarial defect model with, green fluorescent protein (GFP) labeled osteocytes, the osteocytes were observed to migrate into the matrix as early as 24 hours. They were also identified in the matrix up to 14 days after transplantation. Together with these findings, we conclude that DWJM can be used as a 3D porous, bioactive and biocompatible scaffold for tissue engineering and regenerative medicine applications.

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Section: Methodsmentioning

confidence: 99%

Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications

et al. 2017

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“…In 2013, two researchers, demonstrated that it is possible to obtain cells with an expression of cytokeratin 19 and hair-like structures from WJMSC in in vitro conditions. Cytokeratin 19 (CK19) is a marker of bulge stem cells which determines the self-regeneration potential of modified skin [ 85 , 86 ].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Stem Cells in Follicle Regeneration

Owczarczyk‐Saczonek

Krajewska–Włodarczyk

Kruszewska

et al. 2018

Stem Cells International

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Alopecia is caused by a variety of factors which affect the hair cycle and decrease stem cell activity and hair follicle regeneration capability. This process causes lower self-acceptance, which may result in depression and anxiety. However, an early onset of androgenic alopecia is associated with an increased incidence of the metabolic syndrome and an increased risk of the cardiac ischaemic disease. The ubiquity of alopecia provides an encouragement to seek new, more effective therapies aimed at hair follicle regeneration and neoregeneration. We know that stem cells can be used to regenerate hair in several therapeutic strategies: reversing the pathological mechanisms which contribute to hair loss, regeneration of complete hair follicles from their parts, and neogenesis of hair follicles from a stem cell culture with isolated cells or tissue engineering. Hair transplant has become a conventional treatment technique in androgenic alopecia (micrografts). Although an autologous transplant is regarded as the gold standard, its usability is limited, because of both a limited amount of material and a reduced viability of cells obtained in this way. The new therapeutic options are adipose-derived stem cells and stem cells from Wharton's jelly. They seem an ideal cell population for use in regenerative medicine because of the absence of immunogenic properties and their ease of obtainment, multipotential character, ease of differentiating into various cell lines, and considerable potential for angiogenesis. In this article, we presented advantages and limitations of using these types of cells in alopecia treatment.

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“…The Wharton jelly has been extensively studied as a reservoir of mesenchymal stem cells (MSCs) in the field of regenerative medicine. The Wharton jelly MSCs show a differentiation spectrum covering ectodermal and endodermal lineages [10]. To the best of our knowledge, the current case is the first example of hair growth and follicle formation in the umbilical cord.…”

Section: Discussionmentioning

confidence: 99%