Bone Marrow-Derived, Neural-Like Cells Have the Characteristics of Neurons to Protect the Peripheral Nerve in Microenvironment

Guo, Shilei; Zhang, Zhiying; Xu, Yingbin; Yunxia, Zhi; Han, Chang-jie; Zhou, Yuhao; Liu, Fang; Lin, Haiyan; Zhang, Chuansen

doi:10.1155/2015/941625

Cited by 4 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Peripheral nerve injury (PNI) is a common clinical disease [ 1 ] that is usually caused by trauma, compression, ischemic, and metabolic disorders [ 2 ], leading to the loss of sensory and motor neural functions [ 3 – 5 ]. The regeneration of the injured nerve is usually slow and incomplete, while patients suffer from pain and from a lower quality of life after PNI [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Exosome-Mediated miR-21 Was Involved in the Promotion of Structural and Functional Recovery Effect Produced by Electroacupuncture in Sciatic Nerve Injury

Liu

Yang

Mou

et al. 2022

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Purpose. Our study is aimed at investigating the mechanism by which electroacupuncture (EA) promoted nerve regeneration by regulating the release of exosomes and exosome-mediated miRNA-21 (miR-21) transmission. Furthermore, the effects of Schwann cells- (SC-) derived exosomes on the overexpression of miR-21 for the treatment of PNI were investigated. Methods. A sciatic nerve injury model of rat was constructed, and the expression of miR-21 in serum exosomes and damaged local nerves was detected using RT-qPCR after EA treatment. The exosomes were identified under a transmission electron microscope and using western blotting analysis. Then, the exosome release inhibitor, GW4869, and the miR-21-5p-sponge used for the knockdown of miR-21 were used to clarify the effects of exosomal miR-21 on nerve regeneration promoted by EA. The nerve conduction velocity recovery rate, sciatic nerve function index, and wet weight ratio of gastrocnemius muscle were determined to evaluate sciatic nerve function recovery. SC proliferation and the level of neurotrophic factors were assessed using immunofluorescence staining, and the expression levels of SPRY2 and miR-21 were detected using RT-qPCR analysis. Subsequently, the transmission of exosomal miR-21 from SC to the axon was verified in vitro. Finally, the exosomes derived from the SC infected with the miR-21 overexpression lentivirus were collected and used to treat the rat SNI model to explore the therapeutic role of SC-derived exosomes overexpressing miR-21. Results. We found that EA inhibited the release of serum exosomal miR-21 in a PNI model of rats during the early stage of PNI, while it promoted its release during later stages. EA enhanced the accumulation of miR-21 in the injured nerve and effectively promoted the recovery of nerve function after PNI. The treatment effect of EA was attenuated when the release of circulating exosomes was inhibited or when miR-21 was downregulated in local injury tissue via the miR-21-5p-sponge. Normal exosomes secreted by SC exhibited the ability to promote the recovery of nerve function, while the overexpression of miR-21 enhanced the effects of the exosomes. In addition, exosomal miR-21 secreted by SC could promote neurite outgrowth in vitro. Conclusion. Our results demonstrated the mechanism of EA on PNI from the perspective of exosome-mediated miR-21 transport and provided a theoretical basis for the use of exosomal miR-21 as a novel strategy for the treatment of PNI.

show abstract

Section: Introductionmentioning

confidence: 99%

Exosome-Mediated miR-21 Was Involved in the Promotion of Structural and Functional Recovery Effect Produced by Electroacupuncture in Sciatic Nerve Injury

Liu

Yang

Mou

et al. 2022

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…While there has been much focus on the differentiation of stem cells into gliallike cells, there is also evidence that neural cell-line phenotypes transplanted in the PNS have the potential for repair of nerve defects [18]. NSCs have been employed for PNS regeneration in a number of studies with promising results [21][22][23][24][25][26][27]. Finally, transplanted stem cells release neurotrophic and immunoprotective factors supporting the repair process through paracrine activity [18,27].…”

Section: Introductionmentioning

confidence: 99%

Induced neural stem cell differentiation on a drawn fiber scaffold—toward peripheral nerve regeneration

et al. 2020

View full text Add to dashboard Cite

To achieve regeneration of long sections of damaged nerves, restoration methods such as direct suturing or autologous grafting can be inefficient. Solutions involving biohybrid implants, where neural stem cells are grown in vitro on an active support before implantation, have attracted attention. Using such an approach, combined with recent advancements in microfabrication technology, the chemical and physical environment of cells can be tailored in order to control their behaviors. Herein, a neural stem cell polycarbonate fiber scaffold, fabricated by 3D printing and thermal drawing, is presented. The combined effect of surface microstructure and chemical functionalization using poly-L-ornithine (PLO) and double-walled carbon nanotubes (DWCNTs) on the biocompatibility of the scaffold, induced differentiation of the neural stem cells (NSCs) and channeling of the neural cells was investigated. Upon treatment of the fiber scaffold with a suspension of DWCNTs in PLO (0.039 g l−1) and without recombinants a high degree of differentiation of NSCs into neuronal cells was confirmed by using nestin, galactocerebroside and doublecortin immunoassays. These findings illuminate the potential use of this biohybrid approach for the realization of future nerve regenerative implants.

show abstract

“…So far, mostly neural progenitor and embryonic stem cells have shown to act through direct neuronal replacement while other cells, such as stem cells from adipose tissue, hair, skin, bone marrow and amniotic fluid, seem to be beneficial for their regeneration supportive effect through the production of neurotrophic factors, extracellular matrix components, through growth cone guidance and remyelination [30,35,36,38,39,40,41,45]. Promising cells are also iPSCs that have shown the ability to differentiate into motoneurons in vitro [57,58,59] and to form functional connections with reinnervated muscle in vivo [26].…”

Section: Discussionmentioning

confidence: 99%

“…So far, numerous studies have used various kinds of fetal or self-renewal stem/progenitor cells, including embryonic stem cells [21], Schwann cells [22,23], neural progenitor cells (NPCs) [24,25], induced pluripotent stem cells (iPSCs) [26], and stem cells from bone marrow [27,28,29,30], fat tissue [31,32,33], hair [34,35] and skin [36,37,38]. Many of these studies have shown some modest recovery after peripheral nerve injuries [21,30,35,36,38,39,40,41,42,43,44,45,46]. The underlying mechanisms are still unclear and many of the studies focus on speeding up the axonal regeneration rather than preventing the early muscle atrophy.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of Embryonic Spinal Cord Derived Cells Helps to Prevent Muscle Atrophy after Peripheral Nerve Injury

Ruven

et al. 2017

IJMS

View full text Add to dashboard Cite

Injuries to peripheral nerves are frequent in serious traumas and spinal cord injuries. In addition to surgical approaches, other interventions, such as cell transplantation, should be considered to keep the muscles in good condition until the axons regenerate. In this study, E14.5 rat embryonic spinal cord fetal cells and cultured neural progenitor cells from different spinal cord segments were injected into transected musculocutaneous nerve of 200–300 g female Sprague Dawley (SD) rats, and atrophy in biceps brachii was assessed. Both kinds of cells were able to survive, extend their axons towards the muscle and form neuromuscular junctions that were functional in electromyographic studies. As a result, muscle endplates were preserved and atrophy was reduced. Furthermore, we observed that the fetal cells had a better effect in reducing the muscle atrophy compared to the pure neural progenitor cells, whereas lumbar cells were more beneficial compared to thoracic and cervical cells. In addition, fetal lumbar cells were used to supplement six weeks delayed surgical repair after the nerve transection. Cell transplantation helped to preserve the muscle endplates, which in turn lead to earlier functional recovery seen in behavioral test and electromyography. In conclusion, we were able to show that embryonic spinal cord derived cells, especially the lumbar fetal cells, are beneficial in the treatment of peripheral nerve injuries due to their ability to prevent the muscle atrophy.

show abstract

Bone Marrow-Derived, Neural-Like Cells Have the Characteristics of Neurons to Protect the Peripheral Nerve in Microenvironment

Cited by 4 publications

References 29 publications

Exosome-Mediated miR-21 Was Involved in the Promotion of Structural and Functional Recovery Effect Produced by Electroacupuncture in Sciatic Nerve Injury

Exosome-Mediated miR-21 Was Involved in the Promotion of Structural and Functional Recovery Effect Produced by Electroacupuncture in Sciatic Nerve Injury

Induced neural stem cell differentiation on a drawn fiber scaffold—toward peripheral nerve regeneration

Transplantation of Embryonic Spinal Cord Derived Cells Helps to Prevent Muscle Atrophy after Peripheral Nerve Injury

Contact Info

Product

Resources

About