Bone Marrow-Engrafted Cells after Mice Umbilical Cord Blood Transplantation Differentiate into Osteoblastic Cells in Response to Fracture and Placement of Titanium Screws

Uchida, Kentaro; Ueno, Masaki; Naruse, Keiko; Urabe, Ken; Onuma, Kenji; Sakai, Rina; Itoman, Moritoshi; Takaso, Masashi

doi:10.1538/expanim.61.427

Cited by 4 publications

(4 citation statements)

References 24 publications

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“…Furthermore, Uchida et al . demonstrated that murine UCB transplantation could fully reconstruct not only haematopoietic cells, but also mesenchymal cell lineages able to differentiate into osteoblastic cells in response to environmental specific cues 45 , 46 .…”

Section: Discussionmentioning

confidence: 99%

Neonatal umbilical cord blood transplantation halts skeletal disease progression in the murine model of MPS-I

Azario

Pievani

Priore

et al. 2017

Sci Rep

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Umbilical cord blood (UCB) is a promising source of stem cells to use in early haematopoietic stem cell transplantation (HSCT) approaches for several genetic diseases that can be diagnosed at birth. Mucopolysaccharidosis type I (MPS-I) is a progressive multi-system disorder caused by deficiency of lysosomal enzyme α-L-iduronidase, and patients treated with allogeneic HSCT at the onset have improved outcome, suggesting to administer such therapy as early as possible. Given that the best characterized MPS-I murine model is an immunocompetent mouse, we here developed a transplantation system based on murine UCB. With the final aim of testing the therapeutic efficacy of UCB in MPS-I mice transplanted at birth, we first defined the features of murine UCB cells and demonstrated that they are capable of multi-lineage haematopoietic repopulation of myeloablated adult mice similarly to bone marrow cells. We then assessed the effectiveness of murine UCB cells transplantation in busulfan-conditioned newborn MPS-I mice. Twenty weeks after treatment, iduronidase activity was increased in visceral organs of MPS-I animals, glycosaminoglycans storage was reduced, and skeletal phenotype was ameliorated. This study explores a potential therapy for MPS-I at a very early stage in life and represents a novel model to test UCB-based transplantation approaches for various diseases.

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Section: Discussionmentioning

confidence: 99%

Neonatal umbilical cord blood transplantation halts skeletal disease progression in the murine model of MPS-I

Azario

Pievani

Priore

et al. 2017

Sci Rep

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“…Alternatively there could be an increased concentration of paracrine factors that regulate differentiation. Previously, Uchida et al [ 39 ] used a murine fracture model to demonstrate in vivo bone engraftment and showed that, whilst ossification occurs around the Ti screw, healing was different to that observed within other regions of graft attachment. The authors of this study concluded that ossification was directed by different MSC populations originating from the bone marrow that had been penetrated by the Ti screw; however, there may be contributing influences brought by the microenvironment of the screw.…”

Section: Discussionmentioning

confidence: 99%

Investigating the biological response of human mesenchymal stem cells to titanium surfaces

et al. 2014

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BackgroundWe have investigated the behaviour of a newly characterised population of haemarthrosis fluid-derived human mesenchymal stem cells (HF-hMSCs) with titanium (Ti) surfaces.MethodsHF-hMSCs were seeded onto round cannulated interference (RCI; Smith and Nephew) screws or control Ti discs and cultured under pro-osteogenic conditions.ResultsElectron microscopy showed the attachment and spreading of HF-hMSCs across both Ti surfaces during the early stages of osteogenic culture; however, cells were exclusively localised to the basal regions within the vertex of the Ti screws. In the later stages of culture, an osteoid matrix was deposited on the Ti surfaces with progressive culture expansion and matrix deposition up the sides and the top of the Ti Screws. Quantification of cellular content revealed a significantly higher number of cells within the Ti screw cultures; however, there was no difference in the cellular health. Conversely, alizarin red staining used as both a qualitative and quantitative measure of matrix calcification was significantly increased in Ti disc cultures compared to those of Ti screws.ConclusionsOur results suggest that the gross topography of the metal implant is able to create microenvironment niches that have an influence on cellular behaviour. These results have implications for the design of advanced tissue engineering strategies that seek to use cellular material to enhance biological remodelling and healing following tissue reconstruction.

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“…Stem cells may have a role to aid bone formation in this regard. Several studies have been conducted in vitro and in vivo to test whether stem cell therapy is effective in achieving such a goal 12,[14][15][16][18][19][20][21] .…”

Section: Role Of Stem Cells In Bone Regeneration and Healing (Tables mentioning

confidence: 99%

“…In large massive bone fractures or other types of bone injuries such as osteosarcoma there is a need to resect diseased bone, and despite of intensive fixation of the bony ends, large gap is produced. In such condition the gap area should be reconstructed by a cancellous bone graft or other types of graft based on the experience and preference of the surgeon [12][13][14][15][16][17][18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Role of stem cell therapy in orthopaedic tissue engineering and regenerative medicine: a comprehensive review of the literature from basic to clinical application

Moshiri¹,

Oryan²,

Meimandi-Parizi³

2013

Hard Tissue

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Treatment of soft and hard connective tissues especially those associated with massive tissue loss is technically demanding. Tendon and cartilage have low healing capability. Massive bone injures are often associated with nonunion or malunion and other complications. Treatment of tendon-bone junction is challenging and treatment of osteoarthritis is more palliative than curative. Tissue engineering which composed of three elements including scaffolds, healing promotive factors such as growth factors, and stem cells is an option. Recently stem cell based therapy is much popular due to the encouraging reported results. This review introduced stem cells and discussed their potential application and roles in tissue regenerative medicine. We have focused on the effectiveness of stem cells based therapy on different tissue injuries, including tendon, tendon to bone junction, bone, cartilage and osteoarthritis. In vitro to clinical evidences have been discussed in detail with the aiming to conclude whether stem cell based therapy is a clinically accepted method. This review showed that despite of exploring several sources for the stem cells, the adult mesenchymal stem cells are still the only reliable stem cells in tissue regenerative medicine.In addition, despite of significant improvement in tissue engineering, it seems cell seeding is technically demanding and direct injection of the stem cells is the only reliable method for cell delivery at injured site. Because of controversial results and lack of well-designed clinical trial studies, this review also showed that despite of several animal studies and application of stem cells on various tissue injuries, it is still soon to suggest stem cell therapy as an effective method in restoring morphology and functionality of the injured tissues.

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Bone Marrow-Engrafted Cells after Mice Umbilical Cord Blood Transplantation Differentiate into Osteoblastic Cells in Response to Fracture and Placement of Titanium Screws

Cited by 4 publications

References 24 publications

Neonatal umbilical cord blood transplantation halts skeletal disease progression in the murine model of MPS-I

Neonatal umbilical cord blood transplantation halts skeletal disease progression in the murine model of MPS-I

Investigating the biological response of human mesenchymal stem cells to titanium surfaces

Role of stem cell therapy in orthopaedic tissue engineering and regenerative medicine: a comprehensive review of the literature from basic to clinical application

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