Bone Marrow Mesenchymal Stem Cells Reduce Intestinal Ischemia/Reperfusion Injuries in Rats

Jiang, Haitao; Liu, Qu; Li, Yun; Gu, Lili; Shi, Yichao; Zhang, Jian; Zhu, Weiming; Li, Jieshou

doi:10.1016/j.jss.2009.07.035

Cited by 52 publications

(57 citation statements)

References 39 publications

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“…rEfErENCES brain from structural and functional impairment associated with T-cell tolerance (32). Although MSCs have been proven beneficial when given intramucosally (77)(78)(79) or intraperitoneally (80) in animal models of intestinal HI, in this study we deliberately chose to administer the MSCs intravenously, since it was previously shown that this yielded better engraftment of MSCs to the fetal gut compared with intraperitoneal administration (23), and this could have beneficial effects not only in the gut but in multiple organs that are affected by global HI. In addition, the relative impact of global HI on the fetal gut is essentially lower compared with other vital organs, such as HI-induced cerebral injury.…”

Section: Disclosurementioning

confidence: 99%

Global Hypoxia-Ischemia Induced Inflammation and Structural Changes in the Preterm Ovine Gut Which Were Not Ameliorated by Mesenchymal Stem Cell Treatment

Nikiforou

Willburger

Jong

et al. 2016

Mol Med

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Perinatal asphyxia, a condition of impaired gas exchange during birth, leads to fetal hypoxia-ischemia (HI) and is associated with postnatal adverse outcomes including intestinal dysmotility and necrotizing enterocolitis. Evidence from adult animal models of transient, locally induced intestinal HI has shown that inflammation is essential in HI-induced injury of the gut. Importantly, mesenchymal stem cell (MSC) treatment prevented this HI-induced intestinal damage. We therefore assessed whether fetal global HI induced inflammation, injury and developmental changes in the gut and whether intravenous MSC administration ameliorated these HI-induced adverse intestinal effects. In a preclinical ovine model, fetuses were subjected to umbilical cord occlusion (UCO), with or without MSC treatment, and euthanized 7 d after UCO. Global HI increased the number of myeloperoxidase-positive cells in the mucosa, upregulated messenger RNA (mRNA) levels of interleukin (IL)-1b and IL-17 in gut tissue and caused T-cell invasion in the intestinal muscle layer. Intestinal inflammation following global HI was associated with increased Ki67 + cells in the muscularis and subsequent muscle hyperplasia. Global HI caused distortion of glial fibrillary acidic protein immunoreactivity in the enteric glial cells and increased synaptophysin and serotonin expression in the myenteric ganglia. Intravenous MSC treatment did not ameliorate these HI-induced adverse intestinal events. Global HI resulted in intestinal inflammation and enteric nervous system abnormalities, which are clinically associated with postnatal complications, including feeding intolerance, altered gastrointestinal transit and necrotizing enterocolitis. The intestinal histopathological changes were not prevented by intravenous MSC treatment directly after HI, indicating that alternative treatment regimens for cell-based therapies should be explored. online address: http://www.molmed.org

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Section: Disclosurementioning

confidence: 99%

Global Hypoxia-Ischemia Induced Inflammation and Structural Changes in the Preterm Ovine Gut Which Were Not Ameliorated by Mesenchymal Stem Cell Treatment

Nikiforou

Willburger

Jong

et al. 2016

Mol Med

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“…Ischemia/reperfusion (I/R) intestinal injuries are considered to be events of great clinical relevance which may cause local and remote damage (Gao et al 2006, Jiang et al 2011, Vollmar & Menger 2011, Ben et al 2012. Intestinal ischemia may be classified into acute mesenteric ischemia, chronic mesenteric ischemia, and ischemic colitis which culminate in decreased blood supply to tissues and reduced intestinal oxygen (Rowe & White 2002, Yasuhara 2005, Kaszaki et al 2006.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the high mortality rate, 60-80% (Ritz et al 2005, Boybeyi et al 2014) and morbidity, 60-100% (Oldenburg et al 2004, Jiang et al 2011 caused by intestinal I/R, studies become increasingly more necessary in order to elucidate the mechanisms involved in the event and its possible therapeutic measures (Rocha et al 2012). …”

Section: Introductionmentioning

confidence: 99%

“…The term " multifocal " used to define these types of therapy is related to the fact that they decrease inflammation, reduce risk of infection, and stimulate tissue repair (Manieri & Stappenbeck 2011). Based on these principles, the use of mesenchymal stem cells (MSC) as a treatment of intestinal diseases (Manieri & Stappenbeck 2011, Jiang et al 2011) is being tested.…”

Section: Introductionmentioning

confidence: 99%

“…The activation of these factors will result in inhibition of apoptosis, increased angiogenesis; they stimulate mitosis, proliferation, and differentiation of these cells into intrinsic organ precursors (Jiang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Allogenic mesenchymal stem cell intravenous infusion in reparation of mild intestinal ischemia/reperfusion injury in New Zealand rabbits

et al. 2018

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The present study aimed to evaluate the efficacy of mesenchymal stem cell (MSC) infusion, derived from adipose tissue, on reduction of local and remote tissue damage caused by the event of experimental intestinal I/R in New Zealand breed rabbits. For obtaining, characterization, and cultivation of MSC derived from adipose tissue (MSC-Adp), 3 juvenile animals (four months old) were used. The cells were considered to be viable for therapy after the fourth passage (in vitro phase). For the in vivo stage, 24 young adult animals (six months old) were used, weighing approximately 3.5 kg, in which were randomly divided into two groups, called: IR treated with MSC (I2H/R5H MSC 3D; I2H/R5H MSC 7D); IR treated with PBS (I2H/R5H PBS 3D; I2H/R5H PBS 7D). The animals were anesthetized and submitted to pre-retro-umbilical midline celiotomy. The extramural peri-intestinal marginal artery was located and clamped (predetermined and standardized region) with the aid of a vascular clip, promoting a 2 hour blood flow interruption. After this period, blood flow was reestablished, inhalatory anesthesia was suspended, and the animals awaken. After 5 hours of reperfusion, the treatments were performed by intravenous infusion according to the experimental groups. The animals were evaluated 72 hours and seven days after the treatment as for the macroscopic appearance (color and peristaltism) of the jejunal segment, and by histological evaluation of the ischemic segment for the presence or absence of destruction of the intestinal mucosa, edema, bleeding, dilation of lymph vessels, and presence of polymorphonuclear inflammatory cells, both in the mucosa and submucosa. The observed results revealed that the groups treated with MSC-Adp obtained smaller mucosal and submucosal lesions when compared to the groups treated with PBS. Also, MSC-Adp treated groups obtained controlled inflammatory response and higher mitotic rate, outcomes related to the therapeutic potential of MSC. Infusion of stem cells attenuated the lesions caused by intestinal I/R in both MSC groups when compared to the group treated with PBS.

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The role of mesenchymal stromal cells in the repair of acute organ injury

Temnov

Вагабов

Sklifas

et al. 2016

The Biology and Therapeutic Application of Mesenchymal Cells

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Bone Marrow Mesenchymal Stem Cells Reduce Intestinal Ischemia/Reperfusion Injuries in Rats

Cited by 52 publications

References 39 publications

Global Hypoxia-Ischemia Induced Inflammation and Structural Changes in the Preterm Ovine Gut Which Were Not Ameliorated by Mesenchymal Stem Cell Treatment

Global Hypoxia-Ischemia Induced Inflammation and Structural Changes in the Preterm Ovine Gut Which Were Not Ameliorated by Mesenchymal Stem Cell Treatment

Allogenic mesenchymal stem cell intravenous infusion in reparation of mild intestinal ischemia/reperfusion injury in New Zealand rabbits

The role of mesenchymal stromal cells in the repair of acute organ injury

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